Root canal irrigants influence the hydrophobicity and adherence of Staphylococcus epidermidis to root canal dentin: an in vitro study

Abstract

ObjectivesTo determine the effect of root canal irrigants on the hydrophobicity and adherence of Staphylococcus epidermidis (S. epidermidis) to root canal dentin in vitro.Materials and MethodsRoot dentin blocks (n = 60) were randomly divided into 4 groups based on the irrigation regimen: group 1, saline; group 2, 5.25% sodium hypochlorite (NaOCl); group 3, 5.25% NaOCl followed by 17% ethylenediaminetetraacetic acid (EDTA); group 4, same as group 3 followed by 2% chlorhexidine (CHX). The hydrophobicity of S. epidermidis to root dentin was calculated by cell surface hydrophobicity while the adherence was observed by fluorescence microscopy, and bacteria were quantified using ImageJ software (National Institutes of Health). Statistical analysis of the data was done using Kruskal-Wallis test and Mann-Whitney U test (p = 0.05).ResultsThe hydrophobicity and adherence of S. epidermidis to dentin were significantly increased after irrigating with group 3 (NaOCl-EDTA) (p < 0.05), whereas in group 4 (NaOCl-EDTA-CHX) both hydrophobicity and adherence were significantly reduced (p < 0.05).ConclusionsThe adherence of S. epidermidis to dentin was influenced differently by root canal irrigants. Final irrigation with CHX reduces the bacterial adherence and may impact biofilm formation.

Quaternary ammonium silane (k21) based intracanal medicament triggers biofilm destruction

“…The microbial adhesion to solid surface is the first step for biofilm formation [ 63 ]. Studies showed that chemical agents have the ability to change the physicochemical properties of dentine which can influence the nature of adherence of microorganism to dentine [ 37 , 64 ]. Hence investigating the influence of 2%K21 on the adherence of E. faecalis and C. albicans to root canal dentine would be of importance.…”

“…Dentin blocks were stained and counterstained with acridine orange (AO) (Sigma-Aldrich, St. Louis, MO, USA) and 0.1% crystal violet (Sigma, St. Louis, MO, USA) for 15 and 5 min respectively. The blocks were observed under fluorescent microscope (Eclipse TiU, Nikon, Tokyo, Japan) [ 37 , 38 ]. An independent blinded observer examined five different dentin blocks at three randomly selected areas.…”

Análise dos irrigantes endodônticos contra o biofilme de Enterococcus faecalis: uma revisão integrativa

“…As características ideais para um bom irrigante são: a destruição arquitetura do biofilme; a lubrificação do canal radicular; a desinfecção eficaz; a biocompatibilidade com o complexo dentino-pulpar; o efeito antibacteriano e descalcificante. Entretanto, as soluções atualmente comercializadas não atendem a todos estes requisitos simultaneamente 5 .…”

“…As soluções irrigantes mais utilizadas no estágio de limpeza química no tratamento do canal radicular são hipoclorito de sódio (NaOCl), ácido etilenodiaminotetracético (EDTA) e clorexidina (CHX). As soluções nanoparticuladas de prata e de óxido de zinco, juntamente com os irrigantes a base de vegetais, têm ganhado o foco como alternativas aos irrigantes convencionais, sendo desenvolvidos estudos com o intuito comprovar a eficiência destes [4][5][6] .…”