The Surface Charge of Visible Particulate Matter Predicts Biological Activation in Human Bronchial Epithelial Cells

Veronesi, Bellina; Haar, Colin de; Lee, Lseng; Oortgiesen, M.

doi:10.1006/taap.2001.9341

Cited by 87 publications

(65 citation statements)

References 47 publications

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“…to charged particles (Veronesi, de Haar et al 2002), metal-rich particles , urban dust (Smith and Aust 1997), diesel particulate (Steerenberg, Zonnenberg et al 1998), carbon nanotubes (Pulskamp, Diabate et al 2007), endotoxin (Schulz, Farkas et al 2002), mineral dusts (Hetland, Refsnes et al 2000;Øvrevik, Myran et al 2005), and soilderived dusts (Veranth, Moss et al 2006). The immortalized cell lines are especially useful for mechanistic studies of particle toxicology because they allow experimental replication over extended time periods, and the cells can be manipulated by molecular biology techniques.…”

Section: Conflict Of Interest Statementmentioning

confidence: 99%

“…This study was motivated by our initial difficulties in replicating published experiments that described induction of cytokines by BEAS-2B, A549 and RAW264.7 cells in response to treatment with various metal-containing agonists such as combustion ash and soluble vanadium. Specifically, our attempts to use soluble vanadium or synthetic residual oil fly ash mixtures (Samet, Stonehuerner et al 1997) as a positive control in our studies of transition metals in coal fly ash and other types of particulate matter produced confusing results even though these materials had been reported to induce proinflammatory signaling in BEAS-2B (Kennedy, Ghio et al 1998;Samet, Stonehuerner et al 1997;Veronesi, de Haar et al 2002) and NHBE cells (Carter, Ghio et al 1997) and systemic responses in animals (Costa and Dreher 1997;Nurkiewicz, Porter et al 2004;Prichard, Ghio et al 1996). Our preliminary experiments measuring both IL-6 and IL-8 suggested that some cell culture models appeared sensitive to metals and resistant to lipopolysaccharide (LPS) while others showed the reverse pattern.…”

Section: Conflict Of Interest Statementmentioning

confidence: 99%

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Effects of cell type and culture media on Interleukin-6 secretion in response to environmental particles

Veranth

Cutler

Kaser

et al. 2008

Toxicology in Vitro

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Cultured lung cells provide an alternative to animal exposures for comparing the effects of different types of air pollution particles. Studies of particulate matter in vitro have reported proinflammatory cytokine signaling in response to many types of environmental particles, but there have been few studies comparing identical treatments in multiple cell types or identical cells with alternative cell culture protocols. We compared soil-derived, diesel, coal fly ash, titanium dioxide, and kaolin particles along with soluble vanadium and lipopolysaccharide, applied to airway-derived cells grown in submerged culture. Cell types included A549, BEAS-2B, RAW 264.7, and primary macrophages. The cell culture models (specific combinations of cell types and culture conditions) were reproducibly different in the cytokine signaling responses to the suite of treatments. Further, Interleukin-6 (IL-6) response to the treatments changed when the same cells, BEAS-2B, were grown in KGM versus LHC-9 media or in media containing bovine serum. The effect of changing media composition was reversible over multiple changes of media type. Other variables tested included culture well size and degree of confluence. The observation that sensitivity of a cell type to environmental agonists can be manipulated by modifying culture conditions suggests a novel approach for studying biochemical mechanisms of particle toxicity.

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Section: Conflict Of Interest Statementmentioning

confidence: 99%

Section: Conflict Of Interest Statementmentioning

confidence: 99%

Effects of cell type and culture media on Interleukin-6 secretion in response to environmental particles

Veranth

Cutler

Kaser

et al. 2008

Toxicology in Vitro

View full text Add to dashboard Cite

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“…In cell biology, the concept of Zeta potential has been used to study cell biological activation, cell agglutination and cell adhesion which are related to cell surface charge properties (Veronesi et al 2002;Fontes et al 2006;Lin et al 2006). In particular, Altankov et al stated that the Zeta potential might be a critical parameter for cellular interaction (Altankov et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Zeta potential: a surface electrical characteristic to probe the interaction of nanoparticles with normal and cancer human breast epithelial cells

Zhang

Yang

Portney

et al. 2007

Biomed Microdevices

373

215

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We demonstrate the use of surface Zeta potential measurements as a new tool to investigate the interactions of iron oxide nanoparticles and cowpea mosaic virus (CPMV) nanoparticles with human normal breast epithelial cells (MCF10A) and cancer breast epithelial cells (MCF7) respectively. A substantial understanding in the interaction of nanoparticles with normal and cancer cells in vitro will enable the capabilities of improving diagnostic and treatment methods in cancer research, such as imaging and targeted drug delivery. A theoretical Zeta potential model is first established to show the effects of binding process and internalization process during the nanoparticle uptake by cells and the possible trends of Zeta potential change is predicted for different cell endocytosis capacities. The corresponding changes of total surface charge of cells in the form of Zeta potential measurements were then reported after incubated respectively with iron oxide nanoparticles and CPMV nanoparticles. As observed, after MCF7 and MCF10A cells were incubated respectively with two types of nanoparticles, the significant differences in their surface charge change indicate the potential role of Zeta potential as a valuable biological signature in studying the cellular Biomed Microdevices

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“…21 Inhalation studies of normal and emphysematous rats revealed no pulmonary toxicity of MSHA. 17 MSHA also has been used as a negative control particle in a number of in vitro studies, including studies on pulmonary macrophage activation by complement, 22 activation of human bronchial epithelial cells, 23 and induction of the lung myofibroblast PDGF receptor system. 24 These results concur with previous findings that characterize MSHA as benign.…”

Section: Discussionmentioning

confidence: 99%

“…27 Human bronchial epithelial cells exposed to a variety of particulates evidenced biological activation in vitro associated with surface charge of the particles, which led to a predictable initiation of airway inflammation. 23 It is possible, but unlikely, that sufficient force is generated in the HAPC's virtual impactors to produce this phenomenon. However, no inflammatory response was produced in our experiment by passing MSHA through the HAPC.…”

Section: Discussionmentioning

confidence: 99%

Does the Harvard/U.S. Environmental Protection Agency Ambient Particle Concentrator Change the Toxic Potential of Particles?

Savage

Lawrence

Katz

et al. 2003

Journal of the Air & Waste Management Association

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The Surface Charge of Visible Particulate Matter Predicts Biological Activation in Human Bronchial Epithelial Cells

Cited by 87 publications

References 47 publications

Effects of cell type and culture media on Interleukin-6 secretion in response to environmental particles

Effects of cell type and culture media on Interleukin-6 secretion in response to environmental particles

Zeta potential: a surface electrical characteristic to probe the interaction of nanoparticles with normal and cancer human breast epithelial cells

Does the Harvard/U.S. Environmental Protection Agency Ambient Particle Concentrator Change the Toxic Potential of Particles?

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