The suppressive role of nanoencapsulated chia oil against DMBA-induced breast cancer through oxidative stress repression and tumor genes expression modulation in rats

Abstract

Background Chia oil is high in omega-3 fatty acids, which have been linked to a lower risk of many diseases, including cancer. Oil encapsulation is a method that holds promise for maintaining oil content while enhancing solubility and stability. The purpose of this study is to prepare nanoencapsulated Chia oil and investigate its suppressive effects on rat chemically induced breast cancer. Methods The oil was extracted from commercial Chia seeds and their fatty acids were analyzed using Gas Chromatography–mass spectrometry (GC/MS). Sodium alginate was used as a loading agent to create the Chia oil nanocapsules. The DPPH assay was used to assess the oil nanocapsules' capacity to scavenge free radicals. Breast cancer induction was done by single dose subcutaneously administration of 80 mg/kg dimethylbenz (a) anthracene (DMBA). Models of breast cancer were given Chia oil nanocapsules orally for one month at doses of 100 and 200 mg/kg. Through measuring intracellular reactive oxygen species (ROS) and protein carbonyl, assessing the gene expression of tumor suppressor genes (BRCA 1 & 2, TP53), and conducting histopathological analysis, the suppressive effect of Chia oil nanocapsules was examined. Results The increase in ROS and PC levels brought on by DMBA was significantly decreased by the administration of Chia oil nanocapsules. In tumor tissue from rats given Chia oil nanocapsules, the mRNA expression levels of BRCA1, BRCA2, and TP53 were controlled Histopathological analysis clarified that the tissue architecture of breast tumors was improved by nanocapsules management. Conclusions These findings demonstrate the ability of Chia oil nanocapsules to inhibit cancer cells in the rat breast.

Formulation of quinoa oil-alginate loaded nanoemulsion and its anticancer efficacy as a therapy for chemically induced breast cancer

“…The quinoa oil fatty acid methyl ester analysis by GC-MS spectrometry was conducted as in El Makawy et al [24].…”

The Role of Obesity in Breast Cancer Pathogenesis

“…Multiple studies further prove that a reduction in oxidative stress improves breast cancer prognosis, though the type of antioxidant is relevant [ 82 ]. Nanoencapsulated antioxidants, such as resveratrol, a natural polyphenol mostly found in the skin of grapes [ 83 ], and chia oil, an omega-3 fatty acid, have been shown to reduce progression of breast cancer via regulation of oxidative stress [ 84 , 85 , 86 ], with the former being tested in humans and the latter being tested in murine models. In the context of oxidative stress as a modifiable environmental factor, a randomized clinical trial has shown that long-term yoga significantly reduces inflammatory cytokines and oxidative stress specifically in breast cancer patients [ 87 ].…”

Nanotechnology and Its Prospective Role in Using Bioactive Compounds to Fight Covid 19 Infection in This Era

“…Recently, many studies have shown the importance of nano-encapsulation of chia seed extract, its impact on human health, and its use in the pharmaceutical and food industries. El-makawy et al (2022) studied the effect of nano-encapsulated chia oil of inhibition against DMBA-induced breast cancer through tumor gene expression modulation and oxidative stress repression in rats. The results showed that chia oil nano-capsules improved the tissue architecture of breast tumors and inhibited cancer cells in the rat breast.…”