The suppressive effect of a supernate from concanavalin A-activated human lymphocytes: Effects of concanavalin A-activated lymphocytes and their supernates on cytotoxic and mixed lymphocyte reactions

Kaufman, Donald B.; Carnaud, Claude; Stach, J L; Bach, Jean‐François

doi:10.1016/0008-8749(79)90323-x

Cited by 20 publications

(7 citation statements)

References 28 publications

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“…Despite some evidence to the contrary (21), it appears that suppression by these lectin-activated cells involves, at least in part, the elaboration of several short-range factors that inhibit target cell function. Indeed, ConA-activated human lymphocyte supernatants were shown to contain suppressor factors that inhibit the mixed leukocyte reaction (16,30), mitogen-and antigen-stimulated T-cell proliferation, and B-cell immunoglobulin production (13,29).…”

Section: Discussionmentioning

confidence: 99%

Immunity to herpes simplex virus type 2: recurrent lesions are associated with the induction of suppressor cells and soluble suppressor factors

Iwasaka¹,

Sheridan²,

Aurelian³

1983

Infect Immun

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Cell-mediated immunity to herpes simplex virus type 2 was investigated in infected inbred strain 13/N guinea pigs with (45%), and without, a history of recurrent herpetic disease (A. D. Donnenberg, E. Chaikof, and L. Aurelian, Infect. Immun. 30:99-109, 1980). Induction of suppressor cells capable of reducing the proliferative response of herpes simplex virus type 2-stimulated immune lymphoid cells was demonstrated in spleen cells from animals with a history of recurrent disease at recrudescence and convalescence but not in spleen cells from quiescent animals or from animals without a history of recurrent herpetic disease (seropositive controls). Suppressor cells were also detected in the peripheral blood but only from three of seven studied animals, and only at recrudescence. In addition to inhibitory cell-cell interactions, the herpes simplex virus type 2-activated regulatory cells of animals with recrudescent herpetic lesions elaborated soluble suppressor factors affecting lymphocyte proliferation. Suppression mediated by suppressor factors was observed only when suppressor factors were added at an early stage of in vitro culture and was reversed by medium exchange throughout the 6 days of culture. Sephadex chromatography revealed the presence of factors capable of differentially modulating the proliferative response of herpes simplex virus-stimulated immune cells and concanavalin A-stimulated normal lymphoid cells.

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Section: Discussionmentioning

confidence: 99%

Immunity to herpes simplex virus type 2: recurrent lesions are associated with the induction of suppressor cells and soluble suppressor factors

Iwasaka¹,

Sheridan²,

Aurelian³

1983

Infect Immun

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“…As early as 1979, it was found that supernatants, obtained after ConA stimulation of human lymphocytes isolated from peripheral blood, could suppress mixed lymphocyte cultures (16). Warrington (17) described a factor produced by ConA‐activated T cells, which can suppress the polyclonal immunoglobulin production by B‐lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

Effect of intravenous immunoglobulins on in vitro immunoglobulin formation in patients with antibody immunodeficiency

Krátká

Bártová

Kryštůfková

et al. 2002

APMIS

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Seventeen patients with antibody immunodeficiency (9 subclass IgG immunodeficiencies, 8 common variable immunodeficiencies) and clinically unambiguous immunodeficiency symptomatology participated in the study with 14 healthy donors. The patients were given regular intravenous immunoglobulin (IVIG) infusions with Endobulin. Blood was collected before and 7 days after infusion of the usual IVIG dose. Mononuclear cells were isolated from peripheral blood (PBMC) of the patients by Ficoll-Paque gradient centrifugation. In order to monitor the ability to inhibit or activate polyclonal production of immunoglobulins in vitro, we stimulated PBMC with pokeweed mitogen (PWM) and with a mixture of pokeweed mitogen + concanavalin A (PWM+ConA). We found that an immunomodulatory effect of IVIG persists in vitro even one week after infusion. Polyclonally stimulated IgA and IgM production was suppressed by IVIG infusion mainly in patients with IgG subclass deficiency. The positive stimulatory effect of IVIG infusion on IgG production was confirmed. The IgG production increased in vitro after infusion in both groups of patients and was significantly higher than in healthy donors. Co-stimulation of PWM-stimulated cells with ConA caused an inhibition of immunoglobulin release in normal healthy donors. The infusion supported the capability of ConA to inhibit IgG production in vitro in patients with IgG subclass deficiency, whereas an increase in IgG production with PWM+ConA stimulation after infusion was found in CVID patients. We assume that lymphocytes activated by ConA produce suppressive factors, which can be affected by the IVIG infusion and which can have both an immunostimulatory and an immunosuppressive effect.

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“…These include soluble immune sup pressor supernatant (SISS) [31], soluble suppressor factor [12], inhibitor of DNA synthesis (IDS) [32], sol uble immune response suppressor (SIRS) [33], and SIRS-like supernatant [25], Attemps to identify a sol uble mediator of suppression in the cat were unsuc cessful. The inability to identify a soluble factor could not be attributed to an absence of suppressor cells due to prolonged incubation, because the cells from which the supernatants were derived were found to actively suppress proliferation at the time the supernatants were collected.…”

Section: Discussionmentioning

confidence: 99%

“…Many of the studies investigating the mechanisms of suppression by these cells have identified soluble factors which mediate this response [12,20,25,[31][32][33]. Other studies have focused on identifying the nature of the suppressor cell itself [28,[34][35], Previous work with the inducible suppressor cell system had been done exclusively in humans and mice.…”

Section: Introductionmentioning

confidence: 99%

“…They can inhibit cell-mediated responses, including mitogen-induced [3, 5, 11, 17-19, 22, 23], antigen-induced [24] and al logeneic cell-induced [5,12,14,17,22,25,26] lym phoprolifération, and cell-mediated cytotoxicity [1,10,12,25]. They can inhibit humoral immune re sponses including immunoglobulin synthesis as mea sured by immunofluorescence [4,27,28], ELISA [16], direct plaque-forming cells [15,29,30] and reverse hemolytic plaque assay [21 ].…”

Section: Introductionmentioning

confidence: 99%

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Generation of Concanavalin A-Induced Suppressor Cells in the Cat

Langweiler¹,

Cockerell²

1982

Int Arch Allergy Immunol

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Preactivation of feline peripheral blood mononuclear cells with mitogenic doses of concanavalin A resulted in suppression of proliferation when these cells were cocultured with untreated autologous cells. The degree of suppression was directly proportional to the preactivation time and ratio of preactivated to untreated cells. The generation, but not the activity, of the suppressor cells was dependent on DNA and protein synthesis. Pokeweed mitogen and phytohemagglutinin were also shown to be capable of inducing suppressor cell activity. Suppression was also observed in allogeneic cocultures. Supernatants from concanavalin A-preactivated cell cultures did not suppress proliferation of untreated cells.

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The suppressive effect of a supernate from concanavalin A-activated human lymphocytes: Effects of concanavalin A-activated lymphocytes and their supernates on cytotoxic and mixed lymphocyte reactions

Cited by 20 publications

References 28 publications

Immunity to herpes simplex virus type 2: recurrent lesions are associated with the induction of suppressor cells and soluble suppressor factors

Immunity to herpes simplex virus type 2: recurrent lesions are associated with the induction of suppressor cells and soluble suppressor factors

Effect of intravenous immunoglobulins on in vitro immunoglobulin formation in patients with antibody immunodeficiency

Generation of Concanavalin A-Induced Suppressor Cells in the Cat

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