The <sup>13</sup>C amide I band is still sensitive to conformation change when the regular amide I band cannot be distinguished at the typical position in H<sub>2</sub>O

Increasing numbers of materials have been extensively used as platforms for enzyme immobilization to improve catalytic performance. However, activity of the most of the enzymes was declined after immobilization. Here, we develop a surfactant-activated lipase-inorganic flowerlike hybrid nanomaterials with rational design based on interfacial activation and self-assembly. The resulting surfactant-activated lipase-inorganic hybird nanoflower (activated hNF-lipase) exhibited 460% and 200% higher activity than native lipase and conventional lipase-inorganic hybird nanoflower (hNF-lipase). Furthermore, the activated hNF-lipase displayed good reusability due to its monodispersity and mechanical properties, and had excellent long-time stability. The superior catalytic performances were attributed to both the conformational modulation of surfactants and hierarchical structure of nanoflowers, which not only anchored lipases in an active form, but also decreased the enzyme-support negative interaction and mass-transfer limitations. This new biocatalytic system is promising to find widespread use in applications related to biomedicine, biosensor, and biodiesel.

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“…3a,c , which was unique to the protein secondary structure. Such regions were designated as amides I and II, respectively 30 . In Fig.…”

Section: Resultsmentioning

confidence: 99%

Surfactant-activated lipase hybrid nanoflowers with enhanced enzymatic performance

Cui

Zhao

Liu

et al. 2016

Sci Rep

116

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“…[15] Two regions(1700 cm À 1 to 1600 cm À 1 and 1560 cm À 1 to 1500 cm À 1 ) were unique to the protein secondary structure, which could be designated as amides I and II, respectively. [16] Here the typical absorption peaks of protein-organic nanoflowers occurred at 1633 cm À 1 and 1554 cm À 1 for CONH and 2800-3600 cm À 1 for CH 2 and CH 3 . [17] Meanwhile, compared with the spectrum of Cu 3 (PO 4 ) 2 @BSA, the spectrum of Cu 3 (PO 4 ) 2 @BSA@Ag 3 PO 4 hybrid nanoflowers showed similar adsorption peaks, indicating that Ag 3 PO 4 was immobilized via self-assembly.…”

Section: Chemistryselectmentioning

confidence: 79%

“…While the peaks at 608 and 565 cm −1 could be attributed to the ν4 bending mode of the O=P−O bond, which contributes to the phosphate groups [15] . Two regions(1700 cm −1 to 1600 cm −1 and 1560 cm −1 to 1500 cm −1 ) were unique to the protein secondary structure, which could be designated as amides I and II, respectively [16] . Here the typical absorption peaks of protein‐organic nanoflowers occurred at 1633 cm −1 and 1554 cm −1 for CONH and 2800–3600 cm −1 for CH 2 and CH 3 [17] .…”

Section: Resultsmentioning

confidence: 97%

In Site Generation of Well‐Dispersed Ag₃PO₄ NPs on Protein‐Inorganic Hybrid Nanoflowers with Enhanced Catalytic Performance

Zheng

Jin

et al. 2022

ChemistrySelect

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Developing environmental-friendly and high-performance catalysts hold excellent prospects for pollution control. Herein, a novel nanocatalyst is successfully prepared through a facile insitu deposition method upon bridging copper phosphate and the protein, which allowing uniform Ag 3 PO 4 NPs to be immobilized on both inner and outer hierarchical nanoflowers. Results show that the fabricated hierarchical flower-like architecture of Cu 3 (PO 4 ) 2 @BSA@Ag 3 PO 4 composite demon-strated superior catalytic performance for the hydrogenation of 4-nitrophenol. Moreover, the hybrid nanoflowers hold a high conversion rate (around 95.4 %) and recycling stability over five reaction cycles. Therefore, such a convenient, economical and green approach to synthesize recyclable nanoflowers possesses broad application potential for the treatment of agricultural and industrial wastewater.

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“…A shift in the band regions of 1650–1545 cm −1 and a band at 1601 cm −1 , which characterize the sensitive EV population, enabled to unambiguously distinguish spectra of EVs/s and EVs/DXR. This could be ascribed to a modification of the Amide I band and of the α-helix conformation [ 54 ]. Indeed, in FTIR spectroscopy, different peak positions of the Amide I band in the 1600 to 1700 cm −1 area correlate to different secondary structures of the proteins (i.e., unstructured conformation vs. α-helix conformation) and can be used to monitor conformation changes in proteins/peptides [ 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

FT-IR Spectral Signature of Sensitive and Multidrug-Resistant Osteosarcoma Cell-Derived Extracellular Nanovesicles

Perut

Graziani

Roncuzzi

et al. 2022

Cells

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Osteosarcoma (OS) is the most common primary bone cancer in children and adolescents. Despite aggressive treatment regimens, the outcome is unsatisfactory, and multidrug resistance (MDR) is a pivotal process in OS treatment failure. OS-derived extracellular vesicles (EVs) promote drug resistance to chemotherapy and target therapy through different mechanisms. The aim of this study was to identify subpopulations of osteosarcoma-EVs by Fourier transform infrared spectroscopy (FT-IR) to define a specific spectral signature for sensitive and multidrug-resistant OS-derived EVs. EVs were isolated from sensitive and MDR OS cells as well as from mesenchymal stem cells by differential centrifugation and ultracentrifugation. EVs size, morphology and protein expression were characterized. FT-IR/ATR of EVs spectra were acquired in the region of 400–4000 cm−1 (resolution 4 cm−1, 128 scans). The FT-IR spectra obtained were consistently different in the EVs compared to cells from which they originate. A specific spectral signature, characterized by a shift and a new band (1601cm−1), permitted to clearly distinguish EVs isolated by sensitive and multidrug-resistant OS cells. Our data suggest that FT-IR spectroscopy allows to characterize and define a specific spectral signature for sensitive and MDR OS-derived EVs.

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The ¹³C amide I band is still sensitive to conformation change when the regular amide I band cannot be distinguished at the typical position in H₂O

Cited by 9 publications

References 18 publications

Surfactant-activated lipase hybrid nanoflowers with enhanced enzymatic performance

Surfactant-activated lipase hybrid nanoflowers with enhanced enzymatic performance

In Site Generation of Well‐Dispersed Ag₃PO₄ NPs on Protein‐Inorganic Hybrid Nanoflowers with Enhanced Catalytic Performance

FT-IR Spectral Signature of Sensitive and Multidrug-Resistant Osteosarcoma Cell-Derived Extracellular Nanovesicles

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The 13C amide I band is still sensitive to conformation change when the regular amide I band cannot be distinguished at the typical position in H2O

Cited by 9 publications

References 18 publications

Surfactant-activated lipase hybrid nanoflowers with enhanced enzymatic performance

Surfactant-activated lipase hybrid nanoflowers with enhanced enzymatic performance

In Site Generation of Well‐Dispersed Ag3PO4 NPs on Protein‐Inorganic Hybrid Nanoflowers with Enhanced Catalytic Performance

FT-IR Spectral Signature of Sensitive and Multidrug-Resistant Osteosarcoma Cell-Derived Extracellular Nanovesicles

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Resources

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The ¹³C amide I band is still sensitive to conformation change when the regular amide I band cannot be distinguished at the typical position in H₂O

In Site Generation of Well‐Dispersed Ag₃PO₄ NPs on Protein‐Inorganic Hybrid Nanoflowers with Enhanced Catalytic Performance