The suitability and efficiency of human follicular fluid as a protein supplement in human in vitro fertilization programs

Chi, Hee-Jun; Kim, Dong-Hoon; Koo, Jung-Jin; Chang, Sang-Sik

doi:10.1016/s0015-0282(98)00313-6

Cited by 30 publications

(20 citation statements)

References 22 publications

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“…Like in many other studies [2][3][4][5][6][17][18][19] the results of the present investigation show that the co-culture system generally had beneficial effects on embryo development through increasing the proportion of embryos passing the critical 2-cell stage and developing to blastocysts. Several mechanisms have been suggested to explain the positive effects of co-culture systems on embryonic development and quality.…”

Section: Discussionsupporting

confidence: 83%

“…The present study confirms this finding: the rates of 4-to 8-cell (block-passed) embryos and blastocysts developed from the 1-cell embryos were higher in FF than in Ham's F10. Several recent studies have reported the beneficial effects of follicular fluid used as a supplement or neat form on in vitro maturation of oocytes ( IVM) [9][10][11][12]21], their subsequent fertilization (IVF), and their development in different species [5,12,22]. Hemmings et al (1994) showed the beneficial effects of FF on the in vitro development of human pre-embryos [13].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, FF contains high levels of gonadotrophins that may have enhancing effects on oocyte maturation and embryo development [28]. Various antioxidant agents are present in FF [5], which may explain part o f t h e b e n e f i c i a l e f f e c t s o f F F o n e m b r y o development in vitro. It has also been suggested that FF may stimulate the synthesis of the cell-cycle protein in embryos which in turn supports their development [5].…”

Section: Discussionmentioning

confidence: 99%

“…The varying conditions present in current embryo culture systems may contribute to the poor implantation rate after the transfer of in vitro cultured embryos [1]. Attempts have been made for more than 20 years to improve in vitro culture conditions by changing the media composition and supplements, and by using co-culture systems with helper somatic cells [2][3][4][5][6].…”

mentioning

confidence: 99%

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Follicular Fluid and Cumulus Cells Synergistically Improve Mouse Early Embryo Development In Vitro

Malekshah

Moghaddam

2005

Journal of Reproduction and Development

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Abstract. The development of preimplantation mammalian embryos in vitro is less than optimal. Follicular fluid and cumulus cells have both been used independently, to improve preimplantation embryo quality in culture. This study was undertaken to evaluate the influence of a cumulus cell monolayer in human follicular fluid on mouse early embryo development in vitro. One-cell embryos were obtained from NMRI mice after superovulation with eCG and hCG. Cumulus cells were prepared from mouse egg-cumulus mass. These cells were separated from red blood cells using a Percoll gradient. Follicular fluid was collected from patients undergoing an IVF program during oocyte pick-up. The cumulus cell monolayer was prepared in follicular fluid (FC) and Ham's F10 (HC). Mouse one-cell embryos were cultured in FC, HC, Ham's F10 (HF) and follicular fluid (FF) for 120 h. Only 10.5% of embryos passed the two-cell block in HF. However, the proportions of embryos passing the two-cell block were 23.1%, 21.4% and 68.5% in FF, HC, and FC treatments, respectively; which were significantly different from HF (p<0.05). The differences between FC and the two other treatments were also significant (p<0.001). In FC, 33.7% of one-cell embryos continued to grow to the blastocyst stage whereas only 2.1% and 1.9% of one-cell embryos in FF and HC reached this stage and no embryos developed to blastocyst in HF. The proportion of blastocysts in FC was significantly higher than all other treatments (p<0.001). It can be concluded that follicular fluid and cumulus cells in monolayer form synergistically improve the early embryo culture condition.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Follicular Fluid and Cumulus Cells Synergistically Improve Mouse Early Embryo Development In Vitro

Malekshah

Moghaddam

2005

Journal of Reproduction and Development

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“…The FF has been successfully incorporated in IVM media for bovine [18,19], human [20,21], porcine [22] and equine [23] oocytes. Especially, in buffalos, there are reports that the maturation rate was increased by adding 40% [24] or 100% [25] of high concentration FF to IVM media.…”

Section: Discussionmentioning

confidence: 99%

Attempt at In Vitro Maturation of Minke Whale (Balaenoptera Bonaerensis) Oocytes Using a Portable CO2 Incubator

Iwayama

Ishikawa²,

Ohsumi³

et al. 2005

Journal of Reproduction and Development

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Abstract. The present study was conducted to investigate whether a portable CO2 incubator was effective for in vitro maturation (IVM) of bovine, porcine and minke whale oocytes, and the effect of maturation media supplemented with different hormones; porcine follicle stimulating hormone (pFSH), estradiol-17β (E2), or pregnant mare's serum gonadotropin (PMSG): human chorionic gonadotropin (hCG) for minke whale immature oocytes was also examined. In vitro maturation rates of bovine and porcine oocytes cultured in the portable CO2 incubator were not significantly different from the standard CO2 incubator. In minke whale IVM culture using the portable incubator, the maximum expansion of cumulus mass was observed by pFSH/E2 and PMSG/hCG at the end of IVM culture. Moreover, the IVM culture period was shortened to 28-30 h from 96-120 h previously reported. The proportion of matured oocytes cultured in the medium supplemented with pFSH/E2 (26.7%) was significantly higher (P<0.05) than that with PMSG/hCG (6.9%). The present study indicates that a portable CO2 incubator is a useful device for minke whale IVM culture on a research base ship, and the addition of pFSH/E2 into an IVM medium enhanced cumulus expansion and the proportion of minke whale matured oocytes.

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Proteomic analysis of recurrent spontaneous abortion: Identification of an inadequately expressed set of proteins in human follicular fluid

Kim

Lee

et al. 2006

Proteomics

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Recurrent spontaneous abortion (RSA), defined as the loss of three or more consecutive pregnancies prior to the 20th week of gestation, affects up to 5% of the child-bearing population. To investigate the proteins associated with RSA, the protein expression in human follicular fluid was analyzed using 2-DE. Follicular fluid contains a variety of biologically important proteins for oocyte fertilization and follicle maturation in the mammalian reproductive process. Therefore, it can be used as a provisional source for identifying proteins involved in RSA. In this study, we identified five aberrantly expressed proteins (complement component C3c chain E, fibrinogen gamma, antithrombin, angiotensinogen, and hemopexin precursor) in follicular fluid from RSA patients with MALDI-TOF-MS and nano-LC MS/MS. Western blot analysis confirmed that the protein expression level of fibrinogen gamma and antithrombin was less in follicular fluid from RSA patients than those from normal controls. Semiquantitative RT-PCR and real-time PCR analyses revealed that mRNA level of these coagulation factors was also decreased significantly in chorionic villi of RSA patients compared with normal samples. Taken all together, it is likely that coagulation factors (fibrinogen gamma and antithrombin) play an important role in maintaining the normal pregnancy.

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The suitability and efficiency of human follicular fluid as a protein supplement in human in vitro fertilization programs

Cited by 30 publications

References 22 publications

Follicular Fluid and Cumulus Cells Synergistically Improve Mouse Early Embryo Development In Vitro

Follicular Fluid and Cumulus Cells Synergistically Improve Mouse Early Embryo Development In Vitro

Attempt at In Vitro Maturation of Minke Whale (Balaenoptera Bonaerensis) Oocytes Using a Portable CO2 Incubator

Proteomic analysis of recurrent spontaneous abortion: Identification of an inadequately expressed set of proteins in human follicular fluid

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