The Sugar Content of Cell Walls and Intercellular Spaces in Sugar-Cane Stems and its Relation to Sugar Transport

Hawker, J. S.

doi:10.1071/bi9650959

Cited by 60 publications

(48 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Table I and indicate that free-space concentrations of sucrose can approach the same concentration as total sucrose in the tissue. These findings are in complete accord with a role for cell walls in sucrose transport as suggested previously (6,10). Evidence that the cell wall constitutes the major pathway for movement of sugars between sugarcane parenchyma cells and the conducting tissue is reviewed elsewhere (5).…”

Section: And Discussionsupporting

confidence: 74%

Sugar Accumulation in Sugarcane

Glasziou

Gayler

1972

Plant Physiol.

View full text Add to dashboard Cite

show abstract

Section: And Discussionsupporting

confidence: 74%

Sugar Accumulation in Sugarcane

Glasziou

Gayler

1972

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…The lack of any detectable solute reabsorption may be due to the fact that cut cells comprise a smaller percentage of the total tissue in large slices than in small slices. Therefore, the effect of cut surfaces on the bulk tissue T measurements is probably less in large slices used in this experiment than in small tissue slices used by previous investigators (8,9).…”

Section: Discussionmentioning

confidence: 84%

“…In a previous study, Hawker (9) (5). In the present study, the As of the apoplast solution was either (a) determined psychrometrically from fully hydrated stalk tissue or (b) determined by osmometry following isolation by centrifugation at low RCF.…”

Section: Discussionmentioning

confidence: 99%

Compartmentation of Solutes and Water in Developing Sugarcane Stalk Tissue

Welbaum¹,

Meinzer²

1990

Plant Physiol.

133

108

View full text Add to dashboard Cite

Previous studies have suggested that the apoplast solution of sugarcane stalk tissue contains high concentrations of sucrose, but the accuracy of these reports has been questioned because sucrose leakage from damaged cells may have influenced the results. In this study, the solute potential of the apoplast and symplast of the second (immature), tenth, twentieth, thirtieth, and fortieth internodes of field-grown sugarcane ( determine the extent of solute accumulation in the apoplast as well as the symplast. Two independent methods were used to quantify the water relations of both the symplast and apoplast in terms of i'', ',, and i&p. Specific objectives were to measure 'I, /,, and ,6p for both mature and immature tissue, to determine how the partitioning of solutes and water between the apoplast and symplast changes with development, to identify the solutes present in each compartment, and to determine the relative volume of the apoplast solution in order to assess its importance as a storage compartment for sucrose. MATERIALS AND METHODS Plant Material.Sugarcane (Saccharum spp. hybrid, cv H65-7052) plants were field-grown in a silty-clay loam (Typic Torrox) soil near Waipahu, Oahu, HI. Well-watered 1.5-to 2-year-old plants were excised prior to sunrise when guttation water was present on leaf margins, indicating that the plants were fully hydrated. After excision, plants were immediately sealed in plastic bags and transported to the laboratory. Tissue samples were collected and analyzed on the basis of internode number. The reference point for determining internode number was the TVD leaf (12); the youngest leaf whose blade is fully exposed and not enclosed in the sheaths of older leaves. The first internode was the node below the TVD leaf sheath attachment, and additional internodes were numbered consecutively down the stalk. A 2-year-old sugarcane stalk grown under the conditions described above typically contained approximately 50 internodes. In this study, internodes 2, 10, 20, 30, and 40 were analyzed.Internode tissue sections were prepared for analysis inside a humidified chamber to reduce water loss. The stalk was cut in half at least 1 cm from the node and the epidermis and any pith tissue was removed with a knife, because the fiber content is higher and sucrose concentrations lower in these tissues (3,6

show abstract

“…0-1 cm* g"' f. wt. This falls within the range of 4-20 % determined for other tissues (Hawker, 1965;Minchin & Thorpe, 1984); therefore the apoplastic sugar concentrations for pea leaves might be four-fold higher than the levels measured in IWF. The data in Table 3 suggest that the apoplastic space was reduced slightly in infected leaves.…”

Section: Resultsmentioning

confidence: 94%

Effect of powdery mildew infection on concentrations of apoplastic sugars in pea leaves

Aked

Hall

1993

New Phytologist

View full text Add to dashboard Cite

SUMMARYIntercellular washing fluid (IWF) was extracted by centrifugation from healthy and mildew-infected pea leaves. The level of cytoplasmic contamination of the IWF was estimated to be 0-3 "o for healthy leaves and 0-7 % in mildew-infected leaves as assessed by the presence of malate dehydrogenase activity. The IWF was analyzed for sugars using gas liquid chromatography and the glucose oxidase assay. Changes in the concentrations of glucose, fructose and sucrose showed different trends over an infection time course. Glucose and sucrose concentrations fluctuated over the 7 d period following infection but almost always showed higher levels in infected tissues than in the controls. Fructose concentrations were low and showed a greater increase over 7 d in infected compared to healthy leaves. The pattern of efflux of sucrose from pre-loaded healthy and mildew-infected pea leaf discs suggests that the plasma membranes of cells from infected pea leaves might be more leaky than those of healthy pea leaves. The implications of apoplastic sugar levels for nutritional aspects of the plant-pathogen interaction are discussed.

show abstract

The Sugar Content of Cell Walls and Intercellular Spaces in Sugar-Cane Stems and its Relation to Sugar Transport

Cited by 60 publications

References 4 publications

Sugar Accumulation in Sugarcane

Sugar Accumulation in Sugarcane

Compartmentation of Solutes and Water in Developing Sugarcane Stalk Tissue

Effect of powdery mildew infection on concentrations of apoplastic sugars in pea leaves

Contact Info

Product

Resources

About