The Study on the Method for Maximizing Steviol Glycoside Extract from Stevia Leaves

Witono, Judy Retti; Chandra, Andy

doi:10.1088/1757-899x/742/1/012048

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…Stevia (Rebaudiana bertoni), is an ancient perennial shrub generally found in South America in countries including Paraguay and Brazil (Chang et al, 2005). It contains glycosides considered as low calorie sweeteners, which is about 300 times sweeter than sucrose (Ilca et al, 2017;Lemus-mondaca et al, 2012;Witono and Chandra, 2020). The leaf extract of Stevia possesses many phytochemicals, which include austroinullin, β-carotene, dulcoside, nilacin, rebaudioxides, riboflavin, steviol, stevioside, and tiamin with known antimicrobial properties against many pathogens (Theophilus et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Production and Utilization of Insulin from Stevia Leave (Rebaudiana Bertoni) for the Treatment of Diabetes Mellitus

Abubakar¹,

Gutti²,

Abubakar³

et al. 2023

Int. J. Multidiscipline. Approach. Research. Science.

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Stevia (Rebaudiana bertoni) leave, is an ancient perennial shrub mostly found in South America in countries like Paraguay and Brazil. It contains a low-calorie sweetener, which is about 300 times sweeter than sucrose. Diabetes mellitus is among the common metabolic disorders affecting about 2.8% of the world’s population and is reported to reach 5.4% by the year 2025. In this work, insulin was successfully extracted from Rebaudiana bertoni to serve as an alternative to bovine insulin extracted from animals which is devoid of the risk of transferring infectious diseases from the animals to the diabetic patient as is the case with convectional animal-based insulin. Gas Chromatography-Mass Spectroscopy (GC-MS) and Fourier Transform InfraRed (FTIR) analysis were used to analyze and determine the functional groups available in the isolated compound and the results shows that it has retention time of 14.11 min; abundance/peak area (% PA) of 86.613 %; mass number (m/z) of 413 g/mol; molecular peak ion of 149; fragmentation patterns of 7; and C20H29BrO4 as molecular formula and phthalic acid, 8-bromoctyl butyl ester as IUPAC name. The insulin was tested on three groups of rats (Rattus norvegicus) whereby the glucose level was monitored, and the result shows that the extracted insulin was found to be effective in addition to having antidiabetic effect.

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Section: Introductionmentioning

confidence: 99%

Production and Utilization of Insulin from Stevia Leave (Rebaudiana Bertoni) for the Treatment of Diabetes Mellitus

Abubakar¹,

Gutti²,

Abubakar³

et al. 2023

Int. J. Multidiscipline. Approach. Research. Science.

View full text Add to dashboard Cite

show abstract

“…Stevia (Rebaudiana bertoni) is an ancient perennial shrub mostly found in South America, especially in Paraguay and Brazil (Chang et al, 2005). It contains a glycoside that are low calorie sweeteners, which is about 300 times sweeter than sucrose (Ilca et al, 2017;Lemusmondaca et al, 2012;Witono and Chandra, 2020). The leaf extract of Stevia possesses many phytochemicals, which include austroinullin, β-carotene, dulcoside, nilacin, rebaudioxides, riboflavin, steviol, stevioside, and tiamin, with known antimicrobial properties against many pathogens (Theophilus et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Review: Natural Sweetener from Stevia (Rebaudiana bertoni) Leave

Abubakar

Gutti

Abubakar

2023

ARI

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Stevia (Rebaudiana bertoni) leave is an ancient perennial shrub mostly found in South America (especially Brazil and Paraguay). It contains a low-calorie sweetener, which is about 300 times sweeter than sucrose. Natural sweeteners extracted from plant and artificial or synthetic sweeteners produced from industires, are the two types of sweeteners that are available. Sweetening agents induce and enhance sweet taste which makes it favorable for consumption. Natural sweetening agents are more advisable to use than artificial sweetening agents because they do not have any side effect and is of no harm to health. In this work, the background of stevia leave, uses, and extraction of natural sweetener is reviewed.

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“…Stevia (Rebaudiana bertoni) is an ancient perennial shrub containing glycoside and phytochemicals, and is about 300 times sweeter than sucrose (Chang et al, 2005;Ilca et al, 2017;Lemus-Mondaca et al, 2012;Theophilus et al, 2015;Witono & Chandra, 2020). Stevia extracts including stevioside, have therapeutic properties, antioxidant effect, antimicrobial and antifungal activity (Abou-Arab et al, 2010;Abubakar, et al, 2023a).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Insulin Yield from Extract of Stevia Leave (Rebaudiana Bertoni)

Abubakar,

Dahiru

2023

Int. J. Multidiscipline. Approach. Res. Sci.

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Stevia (Rebaudiana bertoni) leave, is an ancient perennial shrub mostly found in South America such as Paraguay and Brazil, it contains a low-calorie sweetener, which is about 300 times sweeter than sucrose. Insulin is a polypeptide hormone, which has an anabolic property, insulin has a vital role in regulation of carbohydrate metabolism, it also enhances the metabolism of fat and proteins and regulation of certain gene expression. Design expert software 10.0 was used to design the experimental data for the optimization of insulin yield in stevia (Rebaudiana bertoni) leave. Custom design with 3 factor and one response was applied, the independent variables were amount of stevia powder (g), volume of n-hexane (ml) and volume of ethyl acetate (ml) and the response is percentage of insulin yield (%). In this research work, insulin was successfully extracted from stevia (Rebaudiana bertoni) leave and the optimum yield was determined. The optimum extraction conditions for optimum yield of insulin were found to be: 5.668 g of stevia powder, 49.107 ml of n-hexane and 32.386 ml of ethyl acetate.

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The Study on the Method for Maximizing Steviol Glycoside Extract from Stevia Leaves

Cited by 4 publications

References 6 publications

Production and Utilization of Insulin from Stevia Leave (Rebaudiana Bertoni) for the Treatment of Diabetes Mellitus

Production and Utilization of Insulin from Stevia Leave (Rebaudiana Bertoni) for the Treatment of Diabetes Mellitus

Review: Natural Sweetener from Stevia (Rebaudiana bertoni) Leave

Optimization of Insulin Yield from Extract of Stevia Leave (Rebaudiana Bertoni)

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