The study of two alkaliphilic thermophile bacteria of the Anoxybacillus genus as producers of extracellular proteinase

Лаврентьева, Е. В.; Shagzhina, A. P.; Babasanova, O. B.; Dunaevsky, Ya. E.; Намсараев, З. Б.; Бархутова, Д. Д.

doi:10.1134/s0003683809050044

Cited by 9 publications

(2 citation statements)

References 10 publications

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“…Up to date, only a few proteases from Anoxybacillus have been reported. Lavrenteva et al first reported two alkaliphilic and thermophilic Anoxybacillus strains secreted alkaline subtilisin-like serine proteinases ( Lavrenteva et al, 2009 ). Later, a thermo-alkaline protease from Anoxybacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Diversity Analysis of the Extracellular Proteases of Thermophilic Anoxybacillus caldiproteolyticus 1A02591 From Deep-Sea Hydrothermal Vent Sediment

Cheng

Wang²,

Zhang³

et al. 2021

Front. Microbiol.

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Protease-producing bacteria play key roles in the degradation of marine organic nitrogen. Although some deep-sea bacteria are found to produce proteases, there has been no report on protease-secreting Anoxybacillus from marine hydrothermal vent regions. Here, we analyzed the diversity and functions of the proteases, especially the extracellular proteases, of Anoxybacillus caldiproteolyticus 1A02591, a protease-secreting strain isolated from a deep-sea hydrothermal vent sediment of the East Pacific Ocean. Strain 1A02591 is a thermophilic bacterium with a strong protease-secreting ability, which displayed the maximum growth rate (0.139 h–1) and extracellular protease production (307.99 U/mL) at 55°C. Strain 1A02591 contains 75 putative proteases, including 65 intracellular proteases and 10 extracellular proteases according to signal peptide prediction. When strain 1A02591 was cultured with casein, 12 proteases were identified in the secretome, in which metalloproteases (6/12) and serine proteases (4/12) accounted for the majority, and a thermolysin-like protease of the M4 family was the most abundant, suggesting that strain 1A02591 mainly secreted a thermophilic metalloprotease. Correspondingly, the secreted proteases of strain 1A02591 showed the highest activity at the temperature as high as 70°C, and was inhibited 70% by metalloprotease inhibitor o-phenanthroline and 50% by serine protease inhibitor phenylmethylsulfonyl fluoride. The secreted proteases could degrade different proteins, suggesting the role of strain 1A02591 in organic nitrogen degradation in deep-sea hydrothermal ecosystem. These results provide the first insight into the proteases of an Anoxybacillus strain from deep-sea hydrothermal ecosystem, which is helpful in understanding the function of Anoxybacillus in the marine biogeochemical cycle.

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Section: Introductionmentioning

confidence: 99%

Characterization and Diversity Analysis of the Extracellular Proteases of Thermophilic Anoxybacillus caldiproteolyticus 1A02591 From Deep-Sea Hydrothermal Vent Sediment

Cheng

Wang²,

Zhang³

et al. 2021

Front. Microbiol.

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“…These thermozymes have been used in a number of industrial applications as they possess thermal stability to harsh industrial processes at high temperatures (Demirjian et al, 2001). Thermophilic bacilli are the natural source of many thermostable enzymes, and of those from thermophilic bacilli, some thermostable enzymes belonging to species from the genus Anoxybacillus such as amylase (Poli et al, 2006), glucosidase (Cihan et al, 2009), esterase (Colak et al, 2005), aldolase (Ertunga et al, 2007), proteinase (Lavrenteva et al, 2009) and xylanase (Kacagan et al, 2008) have been well characterized.…”

Section: Introductionmentioning

confidence: 99%

Taxonomic Classification of Anoxybacillus Isolates from Geothermal Regions in Turkey by 16S rRNA Gene Sequences and ARDRA, ITS-PCR, Rep-PCR Analyses

Cihan¹

2013

Pol J Microbiol

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A total of 115 endospore-forming bacilli were taken for 16S rRNA gene sequence analyses and clustered among 7 genera. In this paper, the most abundant thermophiles belonging to genus Anoxybacillus with its 53 isolates are presented. The Anoxybacillus species, some of which were producing biotechnologically valuable enzymes, mostly displayed amylolytic and glucosidic activities and the ability of carbohydrate degradation made them superior in number among the other bacilli in these extreme habitats. In comparative sequence analyses, similarities ranged from 91.1% to 99.9% between the isolates and the type strains. Isolates were clustered into eight phylogenetic lineages within the type strains of A. kamchatkensis, A. flavithermus, A. kamchatchensis subsp. asaccharedens, and A. salavatliensis. In addition, C161ab and A321 were proposed as novel species which displayed < 97.0% similarities to their closest relatives. Moreover, their individual AluI, HaeIII, and TaqI ARDRA restriction patterns, ITS-, (GTG)5-, and BOX-PCR fingerprintings generated 27, 28, 31, 35, 40, and 41 clusters, respectively. The twelve type strains and 35 of the isolates showed unique distinctive patterns from all the others at least in two of these analyses. These phenotypic and genomic characters allowed us to differentiate their genotypic diversity from the reference strains.

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Recent discoveries and applications of Anoxybacillus

Goh

Kahar

Chai

et al. 2013

Appl Microbiol Biotechnol

105

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The Bacillaceae family members are a good source of bacteria for bioprocessing and biotransformation involving whole cells or enzymes. In contrast to Bacillus and Geobacillus, Anoxybacillus is a relatively new genus that was proposed in the year 2000. Because these bacteria are alkali-tolerant thermophiles, they are suitable for many industrial applications. More than a decade after the first report of Anoxybacillus, knowledge accumulated from fundamental and applied studies suggests that this genus can serve as a good alternative in many applications related to starch and lignocellulosic biomasses, environmental waste treatment, enzyme technology, and possibly bioenergy production. This current review provides the first summary of past and recent discoveries regarding the isolation of Anoxybacillus, its medium requirements, its proteins that have been characterized and cloned, bioremediation applications, metabolic studies, and genomic analysis. Comparisons to some other members of Bacillaceae and possible future applications of Anoxybacillus are also discussed.

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The study of two alkaliphilic thermophile bacteria of the Anoxybacillus genus as producers of extracellular proteinase

Cited by 9 publications

References 10 publications

Characterization and Diversity Analysis of the Extracellular Proteases of Thermophilic Anoxybacillus caldiproteolyticus 1A02591 From Deep-Sea Hydrothermal Vent Sediment

Characterization and Diversity Analysis of the Extracellular Proteases of Thermophilic Anoxybacillus caldiproteolyticus 1A02591 From Deep-Sea Hydrothermal Vent Sediment

Taxonomic Classification of Anoxybacillus Isolates from Geothermal Regions in Turkey by 16S rRNA Gene Sequences and ARDRA, ITS-PCR, Rep-PCR Analyses

Recent discoveries and applications of Anoxybacillus

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