The study of the interaction mechanism between bovine serum albumin and single-walled carbon nanotubes depending on their diameter and concentration in solid nanocomposites by vibrational spectroscopy

Gerasimenko, Alexander Yu.; Ten, G. N.; Ryabkin, D. I.; Shcherbakova, N. E.; Morozova, Elena A.; Ичкитидзе, Л. П.

doi:10.1016/j.saa.2019.117682

Cited by 23 publications

(16 citation statements)

References 49 publications

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“…Under the influence of IR radiation, it is noticeable that SWCNT and their bundles form a frame structure. It is known that, after synthesis, carbon nanotubes have a large number of defects, mainly vacancy defects [51]. Nanotubes also have high thermal conductivity; however, in the defect region due to the violation of the crystal lattice, thermal conductivity decreases, and hot spots form [53].…”

Section: Discussionmentioning

confidence: 99%

“…Composite materials based on nanotubes and synthetic polymers (polyvinyl alcohol, polymethyl methacrylate, polylactic acid, etc.) were widely studied and showed good results [79,80]; however, the use of natural polymers in combination with nanotubes proved to be more suitable in terms of biocompatibility and the ease of functionalization of nanotubes with biomolecules [51].…”

Section: Discussionmentioning

confidence: 99%

“…The stability and hydrophilicity of such a material can also be controlled by changing the concentration of nanotubes in the composite [65]. In our work, we used a SWCNT concentration of 0.01 wt.%, which, on the one hand, worked well for the frame formation, and on the other hand, did not cause cytotoxicity [51,82,83]. Comprehensive in vivo studies have provided a fairly complete picture of the implantation of PET tape coated with SWCNT/collagen.…”

Section: Discussionmentioning

confidence: 99%

“…Coatings based on chemically cross-linked CNTs of various types showed a positive effect on the osteogenic differentiation of human stem cells [47]. The additional functionalization of CNTs by carboxyl, carbonyl, or amine functional groups, synthetic (polyethylene glycol) or natural (collagen) polymers can affect the physicochemical properties of the material [50,51], its bioinertness, and improve biocompatibility [52]. It was proved by molecular methods simulations [53] and was confirmed in experiments in vitro [54,55] and in vivo [55].…”

Section: Introductionmentioning

confidence: 99%

“…The aim of the study was to investigate the effect of such a coating on the healing quality of the bone channel of laboratory animals (rabbits) during the implantation of an artificial ligament. It is assumed that the SWCNT/collagen coating will contribute to the formation of dense connective tissue (collagen fibers) [51,54,59] and new bone tissue [60,61] at the implant-bone interface compared with the control group without SWCNT for six months of observation.…”

Section: Introductionmentioning

confidence: 99%

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Frame Coating of Single-Walled Carbon Nanotubes in Collagen on PET Fibers for Artificial Joint Ligaments

Gerasimenko

Zhurbina

Cherepanova

et al. 2020

IJMS

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The coating formation technique for artificial knee ligaments was proposed, which provided tight fixation of ligaments of polyethylene terephthalate (PET) fibers as a result of the healing of the bone channel in the short-term period after implantation. The coating is a frame structure of single-walled carbon nanotubes (SWCNT) in a collagen matrix, which is formed by layer-by-layer solidification of an aqueous dispersion of SWCNT with collagen during spin coating and controlled irradiation with IR radiation. Quantum mechanical method SCC DFTB, with a self-consistent charge, was used. It is based on the density functional theory and the tight-binding approximation. The method established the optimal temperature and time for the formation of the equilibrium configurations of the SWCNT/collagen type II complexes to ensure maximum binding energies between the nanotube and the collagen. The highest binding energies were observed in complexes with SWCNT nanometer diameter in comparison with subnanometer SWCNT. The coating had a porous structure—pore size was 0.5—6 μm. The process of reducing the mass and volume of the coating with the initial biodegradation of collagen after contact with blood plasma was demonstrated. This is proved by exceeding the intensity of the SWCNT peaks G and D after contact with the blood serum in the Raman spectrum and by decreasing the intensity of the main collagen bands in the SWCNT/collagen complex frame coating. The number of pores and their size increased to 20 μm. The modification of the PET tape with the SWCNT/collagen coating allowed to increase its hydrophilicity by 1.7 times compared to the original PET fibers and by 1.3 times compared to the collagen coating. A reduced hemolysis level of the PET tape coated with SWCNT/collagen was achieved. The SWCNT/collagen coating provided 2.2 times less hemolysis than an uncoated PET implant. MicroCT showed the effective formation of new bone and dense connective tissue around the implant. A decrease in channel diameter from 2.5 to 1.7 mm was detected at three and, especially, six months after implantation of a PET tape with SWCNT/collagen coating. MicroCT allowed us to identify areas for histological sections, which demonstrated the favorable interaction of the PET tape with the surrounding tissues. In the case of using the PET tape coated with SWCNT/collagen, more active growth of connective tissue with mature collagen fibers in the area of implantation was observed than in the case of only collagen coating. The stimulating effect of SWCNT/collagen on the formation of bone trabeculae around and inside the PET tape was evident in three and six months after implantation. Thus, a PET tape with SWCNT/collagen coating has osteoconductivity as well as a high level of hydrophilicity and hemocompatibility.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Frame Coating of Single-Walled Carbon Nanotubes in Collagen on PET Fibers for Artificial Joint Ligaments

Gerasimenko

Zhurbina

Cherepanova

et al. 2020

IJMS

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Amendments of weld formation in human skin laser soldering

Ryabkin

Meglinski

Gerasimenko

2023

Journal of Biophotonics

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A computational modeling is employed for quantitative assessment of weld formation and area of tissue temperature necrosis during the human skin laser soldering. The evaluation is carried out depending on the components composition of using solders, including bovine serum albumin (BSA), indocyanine green (ICG), and carbon nanotubes (CNTs), as well as the angle of incidence of laser light and its pulse duration. The influence of CNT on the change of thermodynamic characteristics of albumin denaturation and the rate of formation of the laser weld is investigated. The obtained results suggest to limit the duration of laser light pulse by temperature relaxation time to minimize transfer of thermal energy to reduce the heating of human skin tissues. The developed model has a great potential for further optimization of laser soldering of biological tissues technology with greater efficiency in minimizing the weld area.

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Vibrational Properties of (8,8) and (10,10) SWCNTs before and after Albumin Fragment Adsorption: A Molecular Dynamics Study

Raffaini

2022

Macromolecular Symposia

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Single-walled carbon nanotubes (SWNTs) have received great attention for their mechanical, electronic properties, and possible interesting technological applications. Raman spectroscopy has proven to be a sensitive tool for studying the vibrational properties of carbon nanotubes. The frequency of the radial breathing mode (RBM) in the region of 100-350 cm -1 is found to be inversely proportional to the diameter of the nanotube. Using Molecular Mechanics (MM) and Molecular Dynamics (MD) methods conformational and vibrational properties of pure armchair SWCNTs having different diameters and the (10,10) NT after adsorption of an albumin fragment are studied. The distances between carbon atoms in the central part of the nanotube cavity change during MD run at constant temperature (T = 300 K), and the frequencies of this periodic motion are calculated. The conformational and vibrational properties of armchair (10,10) and (8,8) SWCNT, having the diameter equal to 13.56 and 10.85 Å respectively, are here reported. The distances between carbon atoms from opposite sites in the central cavity of the nanotube are calculated during MD runs. Interestingly, for the (10,10) SWCNT the frequency of Raman vibrational mode E 2g around 17 cm -1 is correctly calculated. The frequency of motion due to this motion increases with a decreasing curvature of the armchair CNT. After the adsorption process of an albumin fragment on the (10,10) NT, lower frequencies of motion and lower intensities are calculated. This theoretical work can useful to study possible periodic motion in pure nanomaterials or when they interact with protein fragments, water, or molecules.

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The study of the interaction mechanism between bovine serum albumin and single-walled carbon nanotubes depending on their diameter and concentration in solid nanocomposites by vibrational spectroscopy

Cited by 23 publications

References 49 publications

Frame Coating of Single-Walled Carbon Nanotubes in Collagen on PET Fibers for Artificial Joint Ligaments

Frame Coating of Single-Walled Carbon Nanotubes in Collagen on PET Fibers for Artificial Joint Ligaments

Amendments of weld formation in human skin laser soldering

Vibrational Properties of (8,8) and (10,10) SWCNTs before and after Albumin Fragment Adsorption: A Molecular Dynamics Study

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