The study of bacteria in biopsies from Slovak colorectal adenoma and carcinoma patients

Abstract

The development of colorectal cancer is affected by many factors, especially the intestinal microbiota. However, precise knowledge of bacterial communities associated with the mucosa in various parts of the colon is limited. Herein, we applied the gentamicin protection assay and detected the presence of intracellular bacteria in colorectal biopsies from Slovak patients with colorectal adenoma and carcinoma, and we compared this with healthy controls. The ENTEROtest 24 and MALDI-TOF mass spectrometry identified the cultivated bacteria and results revealed the presence of intracellularly localized Escherichia coli, Proteus mirabilis and Proteus vulgaris in patients with colorectal adenomas and carcinomas. In addition to these species, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis and Bacillus cereus were identified in colorectal biopsies, but these were extracellular. The marked increase in relative abundance of intracellular E. coli in patients with colorectal adenomas and carcinomas was statistically significant compared to controls, and our preliminary data supports E. coli's role as a pro-oncogenic pathogen.

Microbiota Alterations in Precancerous Colon Lesions: A Systematic Review

“…Amongst the selected studies, three used qPCR for quantitative real-time amplifica-tion of bacterial DNA [ 5 , 6 , 7 ] and expressed the results as cycle thresh-old (CT), Log Transformed Copy Number and 2 −ΔΔCt , respectively; two studies [ 8 , 9 ] used metagenomic sequencing; 13 studies used 16S rRNA sequencing and the remaining study [ 10 ] used ENTERO-test 24 plus MALDI-TOF mass spectrometry for bacterial identification. Three additional studies [ 11 , 12 , 13 ] also used qPCR in addition to 16S rRNA gene sequencing.…”

“…In analyzing the studies conducted in Europe, an abundance of Proteobacteria was proven in the Slovak study, which included only ten patients with colorectal adenoma, ten with carcinoma, and nine healthy controls. As opposed to other studies, biopsy specimens were taken from normal mucosa, and both adenomatous and tumorous tissue [ 10 ]. By ENTERO-test 24 and MALDI-TOF mass spectrometry, significant differences were found in the presence of intracellular E. coli [ 10 ].…”

Changes in SNAI1 and VIM gene expression in Caco2 cells after co-cultivation with bacteria from colorectal cancer biopsies

“…Bacteria obtained in our previous study were used for co-cultivation with Caco2 cell lines. Intestinal bacteria of CRC patients were isolated from colorectal biopsies using gentamycin protection assay [18] and maintained and cultured in LB medium. In this experiment, we used Klebsiella pneumoniae, Proteus vulgaris, Proteus mirabilis, Pseudomonas aeruginosa, Enterococcus faecalis and Bacillus cereus.…”

“…In the present study, we mainly focus on altered expression of two genes associated with EMT in human adenocarcinoma cell line Caco2 after co-cultivation with bacteria isolated from colorectal biopsies [18]. We used different types of intracellular bacteria to examine their effect on the SNAI1 and VIM expression, and evaluated the possible role of these bacteria on cancer progression and metastasis.…”

Visualized analysis of trends and hotspots in global oral microbiome research: A bibliometric study

“…[9][10][11] More importantly, a robust interplay between oral microbiome with the gastrointestinal tract has been recently well documented, particularly concerning the role of oral anaerobes in the development and progression of colorectal carcinoma. 10,[12][13][14][15] The early scientific investigations on oral microbial communities can be traced back to around 1680 when van Leeuwenhoek (1632-1723) observed and described several rod-and sphere-shaped microorganisms in the tartar from his teeth with his handmade microscope. 16 This primordial observation signaled the complexity of the oral microbial community.…”