The Structure of Tobacco Mosaic Virus and Its Components

Abstract

An investigation has been made of the optical rotatory dispersion in the region 226 to 366 mmu of tobacco mosaic virus (TMV), the protein subunits isolated therefrom, the rods synthesized from the protein subunits, and the ribonucleic acid (RNA) isolated from TMV. Both TMV and the protein rods show anomalous rotatory dispersion. The RNA shows a Cotton effect with an inflection point around 260 mmu, which is shifted to 272 mmu in concentrated urea solution. A suggested interpretation of the RNA rotatory dispersion is given. The rotatory dispersion of the protein subunits shows an incipient Cotton effect with an inflection point around 293 mmu and the beginnings of a large negative Cotton effect with a trough at 232 mmu. The dispersion data from the protein subunits can be interpreted to indicate that they contain between 25 and 35 per cent alpha-helix. On the basis of recent sequence investigations and the relationship between amino acid composition and polypeptide structure, the helical portion of the protein subunits can be located in the central section of the protein chain.

Small Molecule–Macromolecule Interactions as Studied by Optical Rotatory Dispersion–Circular Dichroism

“…UV Regions-The laboratories of Blout (32,33) and Doty (34,35) in the early 1960's reported ORD and CDmeasurements on polypeptides in theUV region ; Doty's group also measured the CD of polypeptides down to 190 mp, and they calculated the ORD curve using the Kronig-Kramer transform and obtained reasonable agreement with the experimental curve. The use of ORD in protein chemistry is mainly to determine conformations; the currently accepted curves for the various forms, taken from Reference 27, are shown in Fig.…”

Dual‐Controlled Macroscopic Motions in a Supramolecular Hierarchical Assembly of Motor Amphiphiles

“…Functional supramolecular polymers found in living systems are playing vital roles in key biological functions magnificent expressed in controlled transport and movement . While biological systems allow precisely controlled supramolecular polymerization, synthetic supramolecular polymers were implemented with functional tunability and stimuli responsiveness features by delicate organic molecular design .…”

The Impact of Research on the Future of Dental Education: How Research and Innovation Shape Dental Education and the Dental Profession

“…His studies with Franklin were noted by James Watson, Francis Crick, and Maurice Wilkins as leading to the structural models of DNA showing base pairing and the form of a double helix. 70,71 Robert Ledley, another example, was one of the first dental scientists to envision using mathematics and computers to create "a mathematized biology" that enhanced computerized medical (and dental) diagnoses. 8,10,72,73 Ledley recruited Margaret Oakley Dayhoff to join him at the National Biomedical Research Foundation at Georgetown University Medical Center in 1960.…”