The structure of the interstitial surfaces of the epithelial basement membranes of mouse oral mucosa, gingiva and tongue

Abe, Masataka; Osawa, Takeshi

doi:10.1016/s0003-9969(99)00029-1

Cited by 13 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They are salivary serous glands where ducts open at the base of the papillae grooves and secrete a watery liquid that dissolves food contents and makes easy taste perception [ 12 ]. In the order Rodentia, several studies have been undertaken on mice [ 13 , 14 ], cavy [ 15 ], agouti [ 12 ], flying squirrel [ 16 ], American beaver [ 17 ], and lowland paca [ 7 ]. However, studying the lingual papillae of rats may note different shapes on the surface and their connective tissue cores (CTCs) in the treated samples with NaOH solution.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Aspects of the Lingual Papillae and Their Connective Tissue Cores in the Tongue of Rats: A Scanning Electron Microscope Study

Reginato

Bolina

Watanabe

et al. 2014

The Scientific World Journal

View full text Add to dashboard Cite

The aim of the present study was to describe the tridimensional morphological characteristics of the lingual papillae and their connective tissue cores (CTCs) in Sprague Dawley rats. Four types of papillae were reported on the dorsal surface. Filiform papillae were distributed on the tongue surface and after epithelial maceration a conic and multifilamentary shape of the CTCs was revealed. Fungiform papillae were reported on the rostral and middle regions covered by a squamous epithelium. After the removal of the epithelium, the shape of a volcano with the taste orifice at its top was noted. Foliate papillae were composed of five pairs of epithelial folds situated on the lateral-caudal margin of the tongue. After the removal of the epithelium, they were shown to be limited by thin laminar projections. The vallate papilla with an oval shape was present in the caudal region and delimited by an incomplete groove. The morphological characteristics of the lingual papillae of Sprague Dowley rats, three-dimensional SEM images, and the types of papillae on the dorsal surface were similar to those reported previously in other rodent mammals. The maceration technique revealed the details of extracellular matrix with varied shapes form of connective tissue cores.

show abstract

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Aspects of the Lingual Papillae and Their Connective Tissue Cores in the Tongue of Rats: A Scanning Electron Microscope Study

Reginato

Bolina

Watanabe

et al. 2014

The Scientific World Journal

View full text Add to dashboard Cite

show abstract

“…Through its intrinsic biochemical constituents and biomechanical attributes, the BM provides both chemical and mechanical cues that impact cell behavior [1]. Researchers have characterized biophysical properties of the native BM from several different epithelial tissues across a range of species and have found that the BM is a flexible, protein-based substrate comprising nano to submicron scale topographical features [2-13]. …”

Section: Introductionmentioning

confidence: 99%

The ability of corneal epithelial cells to recognize high aspect ratio nanostructures

et al. 2010

View full text Add to dashboard Cite

The basement membrane of the human corneal epithelium comprises topographic features including fibers, pores, and elevations with feature dimensions on the order of 20-400 nm. Understanding the impact of sub-micron and nanotopography on corneal cell behavior will contribute to our understanding of biomechanical cues and will assist in the design of improved synthetic corneal implants. We utilized well defined ridge and groove wave-like nanostructures (wave ordered structures, WOS) of 60-140 pitches (30-70 nm ridge widths) and 200 nm depths to assess human corneal epithelial cell (HCEC) contact guidance and to establish HCEC contact acuity defined as the lower limit in feature dimensions at which cells respond to biomimetic topographic cues. Results using the WOS substrates demonstrate that HCEC contact acuity is in the range of 60 nm pitch for cells in a serum-free basal medium (EpiLife ® ) and in the range of 90 nm pitch for cells in epithelial medium. To further investigate the influence of HCEC contact acuity in the presence of larger topographic cues, we fabricated 70 nm pitch WOS overlaid parallel to the top of the ridges of 800-4000 nm pitch. HCEC cultured in epithelial medium demonstrate a significant increase in the percent of cells aligning to 4000 nm pitch topography with WOS overlay compared to controls (both flat and 70 nm WOS alone) and 4000 nm pitch topography alone. These results highlight the significance of the lower range of basement membrane scale topographic cues on cell response and allow for improved prosthetic design.

show abstract

“…7,8 These findings with SEM provide, due to collagen VII dysfunction, possibly a unique documentation, although under pathologic conditions, on the tridimensional morphology of this important dermoepidermal structure, which was reported only after artificial induction in oral mucosa. 9 …”

Section: Discussionmentioning

confidence: 99%

Scanning electron microscopy of a blister roof in dystrophic epidermolysis bullosa

Almeida

Monteiro

Silva

et al. 2013

An. Bras. Dermatol.

View full text Add to dashboard Cite

In dystrophic epidermolysis bullosa the genetic defect of anchoring fibrils leads to cleavage beneath the basement membrane, with its consequent loss. We performed scanning electron microscopy of an inverted blister roof of a case of dystrophic epidermolysis bullosa, confirmed by immunomapping and gene sequencing. With a magnification of 2000 times a net attached to the blister roof could be easily identified. This net was composed of intertwined flat fibers. With higher magnifications, different fiber sizes could be observed, some thin fibers measuring around 80 nm and thicker ones measuring between 200 and 300 nm.

show abstract

The structure of the interstitial surfaces of the epithelial basement membranes of mouse oral mucosa, gingiva and tongue

Cited by 13 publications

References 21 publications

Three-Dimensional Aspects of the Lingual Papillae and Their Connective Tissue Cores in the Tongue of Rats: A Scanning Electron Microscope Study

Three-Dimensional Aspects of the Lingual Papillae and Their Connective Tissue Cores in the Tongue of Rats: A Scanning Electron Microscope Study

The ability of corneal epithelial cells to recognize high aspect ratio nanostructures

Scanning electron microscopy of a blister roof in dystrophic epidermolysis bullosa

Contact Info

Product

Resources

About