The structure of a β-(1→6)-d-glucan from yeast cell walls

Abstract

By selective enzymolysis, or chemical fractionation, a minor polysaccharide component has been isolated from yeast (Saccharomyces cerevisiae) glucan. This minor component has a degree of polymerization of about 130-140, a highly branched structure, and a high proportion of beta-(1-->6)-glucosidic linkages. The molecules also contain a smaller proportion of beta-(1-->3)-glucosidic linkages that serve mainly as interchain linkages, but some may also be inter-residue linkages.

Consolidation of glycosyl hydrolase family 30: A dual domain 4/7 hydrolase family consisting of two structurally distinct groups

“…A clear distinction is observed between these b-glucosidase/b-xylosidase like enzymes that have exo-function and the phylogenetically related endo-b-1,6-glucanase [17,18]. These later enzymes function in an endo-manner and have been characterized to degrade b-1, 6-glucan, a polysaccharide component of fungal cell walls [19,20].…”

β-Glucans, History, and the Present: Immunomodulatory Aspects and Mechanisms of Action

“…However, the (Bell and Northcote, 1950;Manners and Patterson, 1966). Only after finding that in the yeast cell wall several types of β-glucans exist (Bacon and Farmer, 1968), detailed fractionation of cell wall components and their characterization was made (Manners et al, 1973). It is believed now that the main component of β-glucan from the yeast cell wall is a slightly branched, high-molecular (1→3)-β-D-glucan (DP about 1500, molecular weight ca.…”

Regulation of cell wallβ-glucan assembly:PTC1 Negatively affectsPBS2 Action in a pathway that includes modulation ofEXG1 transcription

“…The/~l,6-glucan is a smaller and highly branched molecule comprised largely of 1,6-1inked residues with a small proportion of 1,3-1inked residues. The average size of//1,6-glucan is approximately 140 200 residues per molecule (Boone et al 1990;Manners et al 1973b).…”