Regulation of cell wallβ-glucan assembly:PTC1 Negatively affectsPBS2 Action in a pathway that includes modulation ofEXG1 transcription

Jiang, Bo; Ram, Arthur F. J.; Sheraton, Jane; Klis, Frans M.; Bussey, Howard

doi:10.1007/bf02191592

Cited by 94 publications

(89 citation statements)

References 48 publications

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“…5). The fact that mutants with increased β-(1,6)-glucan (Jiang et al 1995), but normal levels of β-(1,3)-glucan and chitin, induced Sp-AMP expression suggests a role for other structural forms of glucan (Fig. 6).…”

Section: Discussionmentioning

confidence: 99%

Expression and β-glucan binding properties of Scots pine (Pinus sylvestris L.) antimicrobial protein (Sp-AMP)

et al. 2011

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Scots pine (Pinus sylvestris) secretes a number of small, highly-related, disulfide-rich proteins (Sp-AMPs) in response to challenges with fungal pathogens such as Heterobasidion annosum, although their biological role has been unknown. Here, we examined the expression patterns of these genes, as well as the structure and function of the encoded proteins. Northern blots and quantitative real time PCR showed increased levels of expression that are sustained during the interactions of host trees with pathogens, but not non-pathogens, consistent with a function in conifer tree defenses. Furthermore, the genes were up-regulated after treatment with salicylic acid and an ethylene precursor, 1-aminocyclopropane-1-carboxylic-acid, but neither methyl jasmonate nor H 2 O 2 induced expression, indicating that Sp-AMP gene expression is independent of the jasmonic acid signaling pathways. The cDNA encoding one of the proteins was cloned and expressed in Pichia pastoris. The purified protein had antifungal activity against H. annosum, and caused morphological changes in its hyphae and spores. It was directly shown to bind soluble and insoluble β-(1,3)-glucans, specifically and with high affinity. Furthermore, addition of exogenous glucan is linked to higher levels of Sp-AMP expression in the conifer. Homology modeling and sequence comparisons suggest that a conserved patch on the surface of the globular Sp-AMP is a carbohydrate-binding site that can accommodate approximately four sugar units. We conclude that these proteins belong to a new family of antimicrobial proteins that are likely to act by binding the glucans that are a major component of fungal cell walls.

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Section: Discussionmentioning

confidence: 99%

Expression and β-glucan binding properties of Scots pine (Pinus sylvestris L.) antimicrobial protein (Sp-AMP)

et al. 2011

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“…Single-or double-null mutants in EXG1 and EXG2 have no overt defects, although exg1D cells have slightly elevated levels of b1,6-glucan, and EXG1 overexpressers lower amounts of that polymer, suggesting roles for Exg1 and Exg2 in b-glucan remodeling (Jiang et al 1995;Lesage and Bussey 2006). Ssg1/Spr1 is a sporulationspecific protein (File S9).…”

Section: Known and Predicted Enzymesmentioning

confidence: 99%

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

2012

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The wall gives a Saccharomyces cerevisiae cell its osmotic integrity; defines cell shape during budding growth, mating, sporulation, and pseudohypha formation; and presents adhesive glycoproteins to other yeast cells. The wall consists of b1,3-and b1,6-glucans, a small amount of chitin, and many different proteins that may bear N-and O-linked glycans and a glycolipid anchor. These components become cross-linked in various ways to form higher-order complexes. Wall composition and degree of cross-linking vary during growth and development and change in response to cell wall stress. This article reviews wall biogenesis in vegetative cells, covering the structure of wall components and how they are cross-linked; the biosynthesis of N-and O-linked glycans, glycosylphosphatidylinositol membrane anchors, b1,3-and b1,6-linked glucans, and chitin; the reactions that cross-link wall components; and the possible functions of enzymatic and nonenzymatic cell wall proteins. TABLE OF CONTENTS Abstract 775Introduction 777

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“…It is involved in cell wall -1,3-glucan assembly. 14) In the mushroom Lentinula edodes, the exgA orthologous gene (exg1) expresses specifically in the fruiting body. 13) No other findings as to the role of ExgA, however, have been reported to date.…”

Section: )mentioning

confidence: 99%

The β-1,3-Exoglucanase GeneexgA(exg1) ofAspergillus oryzaeIs Required to Catabolize Extracellular Glucan, and Is Induced in Growth on a Solid Surface

Tamano

Satoh

Ishii

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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Regulation of cell wallβ-glucan assembly:PTC1 Negatively affectsPBS2 Action in a pathway that includes modulation ofEXG1 transcription

Cited by 94 publications

References 48 publications

Expression and β-glucan binding properties of Scots pine (Pinus sylvestris L.) antimicrobial protein (Sp-AMP)

Expression and β-glucan binding properties of Scots pine (Pinus sylvestris L.) antimicrobial protein (Sp-AMP)

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

The β-1,3-Exoglucanase GeneexgA(exg1) ofAspergillus oryzaeIs Required to Catabolize Extracellular Glucan, and Is Induced in Growth on a Solid Surface

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