The Structure and Function of Jembrana Disease Virus Genome

Kusumawati, Asmarani; Wanahari, Tenri Ashari; Tampubolon, Dwades

doi:10.14737/jimb.2307-5465/2.2.26.29

Cited by 7 publications

(11 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Infection of Jembrana disease virus on Bali cattle have caused substantial economic losses for farmers in Indonesia and Australia (Kusumawati et al, 2014a). JDV has spread to almost west region in Indonesia (Soeharsono et al, 1995;Burkala et al, 1999) and has high prevalence which is more than 50% (Kusumawati et al, 2014b).…”

Section: Discussionmentioning

confidence: 99%

“…Immunodiagnostic method based on host humoral response can not be used in early stages of the disease as like the other lentiviruses, JDV infection induces a delayed humoral response and JDV-specific antibodies are not produced in most infected cattle untill 11 weeks post infection (Kusumawati et al, 2014b). Furthermore, antibody-based diagnostic methods do not enable to distinguish JDV-from BIV-infection as the two bovine lentiviruses are antigenically very closely related (Kusumawati et al, 2014a). Distinguishing BIVinfection was only made feasible by using a BIV-specific monoclonal antibody that only recognizes the unique BIV gag epitope, which is not shared by JDV (Kusumawati et al, 2014a).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, antibody-based diagnostic methods do not enable to distinguish JDV-from BIV-infection as the two bovine lentiviruses are antigenically very closely related (Kusumawati et al, 2014a). Distinguishing BIVinfection was only made feasible by using a BIV-specific monoclonal antibody that only recognizes the unique BIV gag epitope, which is not shared by JDV (Kusumawati et al, 2014a). During the acute phase, high titre of infectious JDV viral particles is found in plasma.…”

Section: Discussionmentioning

confidence: 99%

“…Jembrana Disease Virus (JDV) is a lentivirus associated with an acute disease syndrome on Bali cattle (Bos javanicus) in Indonesia and Australia (Kusumawati et al, 2014a). After short latent period, infected cattle show clinical signs of fever, lymphadenopathy and lymphopenia, at which stage high viral titres of 10 8 infectious units/mL are found in the plasma (Kusumawati et al, 2014b;Soeharsono et al, 1990;Soesanto et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the method used has to give accurate result because it is known there is a possibility to cross react with another bovine lentivirus i.e., Bovine Immunodeficiency Virus (BIV) since BIV was genetically and antigenically the most closely related to JDV although the respective associated disease is quite different (Kusumawati et al, 2014a). In this study, we describe the comparison of genomic based-method of reverse transcriptase-polymerase chain reaction (RT-PCR), reverse transcriptase-loop mediated isothermal amplification (RT-LAMP) and dot-blot hybridization to detect JDV genomic material.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The Comparison of RT-LAMP, RT-PCR and Dot-Blot Hybridization for Detection of Jembrana Disease Virus

Kusumawati¹,

Wanahari²,

Tampubolon³

et al. 2015

American Journal of Biochemistry and Biotechnology

Self Cite

View full text Add to dashboard Cite

Jembrana disease virus is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. Infection of Jembrana Disease Virus (JDV) on Bali cattle have caused substantial economic losses for farmers in Indonesia and Australia. In order to control the spread, development of a sensitive detection method is important. In this study, we used three different detection methods based on genomic approach, i.e., reverse transcriptase-polymerase chain reaction (RT-PCR), reverse transcriptase-loop mediated isothermal amplification (RT-LAMP) and dot-blot hybridization to detect JDV. Utilization of pGEX-TM, a recombinant plasmid containing env-tm gene as a positive control showed that RT-LAMP is the most sensitive method compares the two others. It could detect template concentration as low as 10 −6 ng µL −1 or equivalent to 1.52×10 2 plasmid copy number, 100 and 10000 more sensitive than RT-PCR and dot-blot hybridization, respectively.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Comparison of RT-LAMP, RT-PCR and Dot-Blot Hybridization for Detection of Jembrana Disease Virus

Kusumawati¹,

Wanahari²,

Tampubolon³

et al. 2015

American Journal of Biochemistry and Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

Clinical and Pathological Perspectives of Jembrana Disease Virus Infection: A Review

Kusumawati¹,

Wanahari²,

Putri³

et al. 2014

Biosci., Biotechnol. Res. Asia

Self Cite

View full text Add to dashboard Cite

Jembrana disease virus (JDV) is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus). Jembrana disease poses the major concern in Bali cattle industry as it gives rise to significant economic detriment due to mortality of cattle. During the first outbreaks, mortality of approximately 60000 cattles in a year was observed due to JDV infection. The pathology of JDV is unusual for a lentivirus infection as it is associated with an clinically acute, often lethal disease syndrome, and a short incubation period in Bali cattle. Studies of Bali cattle experimentally infected with JDV have provided insights into haematological change, cytopathological response, and immune response in naturally occurring infection. The localization of JDV in various tissues or organs were also had been reported. This review discusses the progression of clinical symptoms and the pathological changes during the development of Jembrana disease.

show abstract

Effect of Ethanol and IPTG on the Recombinant Jembrana Trans-Activator of Transcriptation Protein Expression

et al. 2018

View full text Add to dashboard Cite

Jembrana diseases are caused by Jembrana Diseases Virus (JDV). The previous study showed that Jembrana Trans-Activator of Trancriptation (JTAT) recombinant protein is effective as a vaccine for Jembrana diseases. The production of JTAT protein needs to be optimized to obtain a higher amount of vaccine. High expression of JTAT protein will produce a high vaccine product. This study aimed to examine the effect of the addition of ethanol and IPTG in E. coli media on the expression of JTAT recombinant protein. This research was experimental research with factorial RAL design with a variation factor of ethanol and IPTG. Qualitatively, the induction of each IPTG, ethanol and interaction between the two could induce the expression of JTAT protein and could be identified with SDS-PAGE at ±11.8 kDa. Statistically, the induction of IPTG, ethanol and interaction between the two were not significantly different. Qualitative and quantitative data show that ethanol can induce JTAT protein expression. This result can be used as a preliminary study to test the effectiveness of ethanol as a substitute for IPTG in inducing the recombinant protein expression.

show abstract

The Structure and Function of Jembrana Disease Virus Genome

Cited by 7 publications

References 30 publications

The Comparison of RT-LAMP, RT-PCR and Dot-Blot Hybridization for Detection of Jembrana Disease Virus

The Comparison of RT-LAMP, RT-PCR and Dot-Blot Hybridization for Detection of Jembrana Disease Virus

Clinical and Pathological Perspectives of Jembrana Disease Virus Infection: A Review

Effect of Ethanol and IPTG on the Recombinant Jembrana Trans-Activator of Transcriptation Protein Expression

Contact Info

Product

Resources

About