The stromal composition of malignant lymphoid aggregates in bone marrow: variations in architecture and phenotype in different B‐cell tumours

Vega, Francisco; Medeiros, L. Jeffrey; Lang, Wen Hua; Mansoor, Adnan; Bueso-Ramos, Carlos; Jones, Dan

doi:10.1046/j.1365-2141.2002.03497.x

Cited by 43 publications

(36 citation statements)

References 45 publications

(44 reference statements)

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“…Overexpression of CD23 has also been studied as a potential marker of malignant aggregates [8]. Others have noted that in normal bone marrow, immunohistochemical stains against low-affinity nerve growth factor receptor highlight a fine network of adventitial reticular cells, whereas the nodular aggregates of various lymphomas are characterized by the distortion of the adventitial reticular cell network and a change in the expression of low-affinity nerve growth factor receptor [20].…”

Section: Discussionmentioning

confidence: 99%

Benign lymphoid aggregates in the bone marrow: distribution patterns of B and T lymphocytes

et al. 2013

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Summary Benign lymphoid aggregates are seen in only a minority of bone marrow specimens, but their distinction from non-Hodgkin lymphoma, particularly B-cell lymphomas, can represent a diagnostic challenge. Although criteria have been proposed to help distinguish between benign and malignant aggregates, a detailed description of the distribution patterns of B and T lymphocytes within benign lymphoid aggregates has not been investigated. One hundred thirty-seven cases of bone marrow specimens containing benign aggregates were studied with a panel of immunostains. A subset of these cases was also examined for immunoglobulin gene rearrangements by polymerase chain reaction. The aggregates were categorized based on size, location (paratrabecular or random), presence of infiltrating edges, and distribution of lymphoid cell populations. In addition, we examined 40 cases of bone marrow biopsies with documented malignant lymphoid aggregates for comparison purposes. We report that the distribution of B and T lymphocytes within lymphoid aggregates may serve as a useful criterion to aid in the separation between benign and malignant aggregates. When aggregates exhibit a predominance of T cells, consist of a central core of T cells surrounded by a rim of B cells, or have a mixed distribution of B and T cells, they are more likely to be benign. On the other hand, an increased likelihood of malignancy occurs when aggregates exhibit a predominance of B cells or consist of a central core of B cells surrounded by a rim of T cells (excluding germinal center formation), and assessing other features worrisome of malignancy (large aggregate size, presence of infiltrative edges, cellular atypia, and paratrabecular location, among others) is warranted.

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Section: Discussionmentioning

confidence: 99%

Benign lymphoid aggregates in the bone marrow: distribution patterns of B and T lymphocytes

et al. 2013

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“…[17][18][19][20][21] Collectively, the various components of the bone marrow microenvironment play an active role in hematopoiesis by producing numerous cytokines, inhibitory factors, adhesion molecules, and contact matrix that promote hematopoietic cell production and osteogenesis. 20 Alterations in bone marrow stroma can occur in both benign and malignant disorders, [21][22][23][24][25] including metastatic tumors involving the bone marrow. 23 They are particularly pronounced and have been better described in myeloid neoplasms associated with bone marrow fibrosis such as primary myelofibrosis.…”

Section: Discussionmentioning

confidence: 99%

The stromal composition of mast cell aggregates in systemic mastocytosis

et al. 2009

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Systemic mastocytosis is a stem cell disorder characterized histologically by the presence of multifocal compact aggregates of mast cells in at least one extracutaneous organ with or without evidence of skin lesions. The mast cell aggregates are accompanied by fibrosis, which is often significant. However, in spite of its frequent occurrence and severity, little is known about its characteristics. In this study, we evaluated the composition of the fibrotic mast cell aggregates by studying eight bone marrow biopsies and two spleens involved by systemic mastocytosis, and compared the findings with those observed in other fibrotic bone marrow disorders such as primary myelofibrosis and metastatic malignancy. Histochemistry and immunohistochemistry were used to evaluate: (a) extracellular matrix (reticulin, trichrome, collagen IV, laminin); (b) stromal reticulum cells (low-affinity nerve growth factor receptor); (c) presence of myofibroblastic differentiation (smooth muscle actin) and (d) microvessel density (CD34). We found that all cases showed marked reticulin and collagen fibrosis. However, unlike primary myelofibrosis and metastatic malignancy, which are usually associated with increased low-affinity nerve growth factor receptor positivity, its expression was low in all cases of systemic mastocytosis. Myofibroblastic differentiation was only focally detected in two of eight bone marrow biopsies. In all cases, the systemic mastocytosis lesions were largely devoid of type IV collagen and laminin. The latter findings were in contrast with those seen in cases of primary myelofibrosis and metastatic malignancy where smooth muscle actin, collagen IV and laminin were expressed in most cases. Also in contrast with the other two conditions, only minimal vascularity was detectable within the fibrotic mast cell lesions. These findings indicate that systemic mastocytosis exhibits a distinct pattern of stromal change, and suggest that the fibrogenetic mechanism in systemic mastocytosis is most likely different from that of other bone marrow neoplasms which are also associated with fibrosis.

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“…However, no data are available in the literature regarding the possible role played by BM endothelial cells in hematological disorders such as NHL. 17 Based on these considerations, the distribution and the immunophenotypical features of CFU-En colonies was also evaluated in the BM of NHL patients with the aim of clarifying whether any difference in CFU-En number could be correlated with (1) the type of the neoplastic clone (T or B cell); (2) the phase of the disease (onset, remission phase after chemotherapy, early and late phases after peripheral blood stem cell transplantation (PBSCT); (3) bone marrow involvement at diagnosis; (4) the grade of the lymphoma (high-intermediate-low grade); (5) the age and the sex; (6) the type of treatment employed (chemotherapy, radiotherapy, PBSCT); (7) number of BM hematopoietic (CFU-C) and mesenchymal (CFU-F) progenitor cells.…”

Section: Non-hodgkin's Lymphoma; Autologous Transplantationmentioning

confidence: 99%

In vitro assessment of bone marrow endothelial colonies (CFU-En) in non-Hodgkin's lymphoma patients undergoing peripheral blood stem cell transplantation

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et al. 2003

Bone Marrow Transplant

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The stromal composition of malignant lymphoid aggregates in bone marrow: variations in architecture and phenotype in different B‐cell tumours

Cited by 43 publications

References 45 publications

Benign lymphoid aggregates in the bone marrow: distribution patterns of B and T lymphocytes

Benign lymphoid aggregates in the bone marrow: distribution patterns of B and T lymphocytes

The stromal composition of mast cell aggregates in systemic mastocytosis

In vitro assessment of bone marrow endothelial colonies (CFU-En) in non-Hodgkin's lymphoma patients undergoing peripheral blood stem cell transplantation

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