The sterol and phospholipid composition of the salt-tolerant yeast Debaryomyces hansenii grown at various concentrations of NaCl

Tunblad-Johansson, Inga; André, Lars; Adler, Lennart

doi:10.1016/0005-2760(87)90177-9

Cited by 29 publications

(7 citation statements)

References 33 publications

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“…This has been reported before for other biological systems [28,34,12,49]. We Debaryomyces hansenii [55] as well as in S. cerevisiae as mentioned above [53,10].…”

Section: Studies On Passive Diffusionsupporting

confidence: 80%

Fps1p channel is the mediator of the major part of glycerol passive diffusion in Saccharomyces cerevisiae: artefacts and re-definitions

Oliveira

Lages

Silva-Graça

et al. 2003

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Glycerol has been shown to cross the plasma membrane of Saccharomyces cerevisiae through (1) a H(+)/symport detected in cells grown on non-fermentable carbon sources, (2) the constitutively expressed Fps1p channel and (3) by passive diffusion. The Fps1p channel has been named a facilitator for mediating glycerol low affinity transport of the facilitated diffusion type. We present experimental evidence that this kinetic is an artefact created by glycerol kinase activity. Instead, the channel is shown to mediate the major part of glycerol's passive diffusion. This is not incompatible with Fps1p's major role in vivo, which has been previously shown to be the control of glycerol export under osmotic stress or in reaction to turgor changes. We also verified that FPS1 overexpression caused an increase in H(+)/symport V(max). Furthermore, yfl054c and fps1 mutants were equally affected by exogenously added ethanol, being the correspondent passive diffusion stimulated. For the first time, to our knowledge, a phenotype attributed to the functioning of YFL054c gene is presented. Glycerol passive diffusion is thus apparently channel-mediated. This is discussed according to glycerol's chemical properties, which contradict the widely spread concept of glycerol's liposoluble nature. The discussion considers the multiple roles that the intracellular levels of glycerol and its pathway regulation might play as a central key to metabolism control.

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“…This has been reported before for other biological systems [28,34,12,49]. We Debaryomyces hansenii [55] as well as in S. cerevisiae as mentioned above [53,10].…”

Section: Studies On Passive Diffusionsupporting

confidence: 80%

Fps1p channel is the mediator of the major part of glycerol passive diffusion in Saccharomyces cerevisiae: artefacts and re-definitions

Oliveira

Lages

Silva-Graça

et al. 2003

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…Thus, over-expression of these genes in H. seosinensis could be linked to membrane sterol homeostasis where less membrane sterol would be required to ensure a fluid membrane at high salt. Interestingly, a decrease in membrane sterol content at increasing salinity was measured directly in the halotolerant yeasts Yarrowia lipolitica and Debaryomyces hansenii (Tunblad-Johansson et al, 1987 ; Andreishcheva et al, 1999 ).…”

Section: Discussionmentioning

confidence: 99%

Adaptations to High Salt in a Halophilic Protist: Differential Expression and Gene Acquisitions through Duplications and Gene Transfers

Harding¹,

Roger²,

Simpson³

2017

Front. Microbiol.

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The capacity of halophiles to thrive in extreme hypersaline habitats derives partly from the tight regulation of ion homeostasis, the salt-dependent adjustment of plasma membrane fluidity, and the increased capability to manage oxidative stress. Halophilic bacteria, and archaea have been intensively studied, and substantial research has been conducted on halophilic fungi, and the green alga Dunaliella. By contrast, there have been very few investigations of halophiles that are phagotrophic protists, i.e., protozoa. To gather fundamental knowledge about salt adaptation in these organisms, we studied the transcriptome-level response of Halocafeteria seosinensis (Stramenopiles) grown under contrasting salinities. We provided further evolutionary context to our analysis by identifying genes that underwent recent duplications. Genes that were highly responsive to salinity variations were involved in stress response (e.g., chaperones), ion homeostasis (e.g., Na+/H+ transporter), metabolism and transport of lipids (e.g., sterol biosynthetic genes), carbohydrate metabolism (e.g., glycosidases), and signal transduction pathways (e.g., transcription factors). A significantly high proportion (43%) of duplicated genes were also differentially expressed, accentuating the importance of gene expansion in adaptation by H. seosinensis to high salt environments. Furthermore, we found two genes that were lateral acquisitions from bacteria, and were also highly up-regulated and highly expressed at high salt, suggesting that this evolutionary mechanism could also have facilitated adaptation to high salt. We propose that a transition toward high-salt adaptation in the ancestors of H. seosinensis required the acquisition of new genes via duplication, and some lateral gene transfers (LGTs), as well as the alteration of transcriptional programs, leading to increased stress resistance, proper establishment of ion gradients, and modification of cell structure properties like membrane fluidity.

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“…Increases in salinity caused increases in the proportion of phosphatidylserine, while those of phophatidylglycerol, phosphatidyl‐ethanolamine, and phosphatidylinositol decreased. Only minor changes were observed in the relative abundance of phosphatidylcholine and cardiolipin178.…”

Section: Promising Characteristics Of Debaryomyces For Biotechnologicmentioning

confidence: 92%

Debaryomyces hansenii — an extremophilic yeast with biotechnological potential

Breuer

Harms

2006

Yeast

270

175

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We illuminate the ecological, physiological and genetic characteristics of the yeast Debaryomyces hansenii in the view of our belief that this metabolically versatile, non-pathogenic, osmotolerant and oleaginous microorganism represents an attractive target for fundamental and applied biotechnological research. To this end, we give a broad overview of extant biotechnological procedures using D. hansenii, e.g. in the manufacture of various foods, and propose research into the heterologous synthesis of a range of fine chemicals.

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The sterol and phospholipid composition of the salt-tolerant yeast Debaryomyces hansenii grown at various concentrations of NaCl

Cited by 29 publications

References 33 publications

Fps1p channel is the mediator of the major part of glycerol passive diffusion in Saccharomyces cerevisiae: artefacts and re-definitions

Fps1p channel is the mediator of the major part of glycerol passive diffusion in Saccharomyces cerevisiae: artefacts and re-definitions

Adaptations to High Salt in a Halophilic Protist: Differential Expression and Gene Acquisitions through Duplications and Gene Transfers

Debaryomyces hansenii — an extremophilic yeast with biotechnological potential

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