The steroidal glycoalkaloid α-tomatine

Roddick, James G.

doi:10.1016/s0031-9422(00)91261-5

Cited by 156 publications

(80 citation statements)

References 106 publications

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“…In tomato the saponin α-tomatine, a steroidal glycoalkaloid, is present in healthy plants, predominantly in leaves, flowers and green fruits (Roddick, 1974). Pathogens able to infect tomato plants contain the enzyme tomatinase to convert the toxic saponin to relatively inactive compounds (α-tomatidine, Osbourne, 1996).…”

Section: Constitutive Defencesmentioning

confidence: 99%

Engineering disease resistance in plants

Stuiver

Custers

2001

Nature

118

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Section: Constitutive Defencesmentioning

confidence: 99%

Engineering disease resistance in plants

Stuiver

Custers

2001

Nature

118

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“…The highest dose used was 30 μg·ml −1 and the inhibition was 78%. According to literature, plants of S. lycopersicum have 10 to 30 mg of a-tomatina per kilo [19]. Considering the maximum concentration of 30 mg per kilo, in our culture media, with 8% of fresh leaves we assume 2 to 2.4 μg per mL.…”

Section: Discussionmentioning

confidence: 99%

Alpha-Tomatin against Witches’ Broom Disease

Gomes¹,

Duarte²,

Andrino³

et al. 2014

AJPS

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Several plants use secondary metabolites against insects and pathogens attack. Among such metabolites known glycoalkaloids are the most studied. Plants from Solanaceae Family are the most abundant on those alkaloids. Despite alfa-tomatine is a known glycoalkaloid, its specific action against Moniliophthora perniciosa, fungi responsible for witches broom disease in cocoa plantations was obtained in this work. Alfa-tomatine was infusion-extracted from Solanum lycopersicum leaves, and its action against the fungi growth was shown on the bioassays. In these tests, M. perniciosa growth was totally inhibited but other fungus tested, the inhibition was not totally, or did not present effect on growth, showing in this way, the specificity of alfa-tomatine to M. perniciosa. The advantages of work with a natural molecule for the fungi control is the stability of the molecule, its resistance to high temperature and pressure and for its use, the simple method of extraction used. The confirmation of the alfa-tomatine molecule was done by High Performance Liquid Chromatography and Mass Spectrometry in comparison to the Sigma standard.

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“…Different types of tomatine, such as β-tomatine, γ-tomatine and δ-tomatine, are generated by the partial hydrolysis of α-tomatine that leads to the loss of different sugar units (11). α-Tomatine provides protection against pathogenic bacteria, fungi and viruses in plants (12).…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanisms underlying the α‑tomatine‑directed apoptosis in human malignant glioblastoma cell lines A172 and U‑118 MG

2017

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Abstract. In the present study, the molecular mechanisms involved in the α-tomatine-induced apoptosis in human glioblastoma cell lines A172 and U-118 MG were investigated. Wright staining and ApopTag assays were conducted to confirm the apoptosis induced by α-tomatine treatment. Fura-2 assay determined an enhancement in free Ca 2+ intracellularly, indicating the occurrence of Ca 2+ -dependent apoptosis induction. Western blot experiments were also performed to predict the apoptosis by measuring the changes in the Bax:Bcl-2 ratio. Increase of calpain activity triggered caspase-12 expression, which in turn further activated caspase-9. In addition, an increase in the ratio of Bax:Bcl-2 accounted for the mitochondrial release of cytochrome c into the cytosol for caspase-3 and caspase-9 activation. Elevated activity of calpain and caspase-3 yielded spectrin breakdown products with 145 and 120 kDa, respectively. Caspase-3 activation further cleaved the inhibitor of caspase activated DNase, while the apoptosis-inducing factor detected in the cytosol suggested that apoptosis was independent of caspase. The apoptosis induction was further supported by decreased expression levels of nuclear factor-κB and increased expression of the inhibitor of nuclear factor, IκBα. In conclusion, the presented experimental results revealed the stimulation of different molecular mechanisms for α-tomatine-mediated apoptosis in A172 and U-118 MG human glioblastoma cell lines.

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The steroidal glycoalkaloid α-tomatine

Cited by 156 publications

References 106 publications

Engineering disease resistance in plants

Engineering disease resistance in plants

Alpha-Tomatin against Witches’ Broom Disease

Molecular mechanisms underlying the α‑tomatine‑directed apoptosis in human malignant glioblastoma cell lines A172 and U‑118 MG

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