Introduction
Gastrointestinal parasitic infections are one of the global health concerns in developing countries like Bangladesh. Among them,
Cryptosporidium
spp. plays an essential role in causing diarrhea, malnutrition, and poor cognitive function, especially in children. This study was conducted to identify the frequency of
Cryptosporidium
cases and other parasitic agents.
Methods
A cross-sectional observational study was conducted among 219 hospitalized children with diarrhea. The conventional microscopic technique was applied for parasitic detection. Particular staining (modified Ziehl-Neelsen) procedure was performed to identify oocysts of
Cryptosporidium
spp
.
A polymerase chain reaction (PCR) was performed to determine the
SSU rRNA
and
gp60
gene of
Cryptosporidium.
Results
Cysts of
Giardia duodenalis
(2.3%), ova of
Ascaris lumbricoides
(1.4%,),
Trichuris trichiura
(0.5%), and both
A. lumbricoides
and
T. trichiura
(0.9%) were identified in samples through wet mount preparation. The distribution of
Cryptosporidium
spp. as detected by the staining method and nested PCR was 1.4% and 4.1%, respectively.
Conclusion
Factors independently associated with
Cryptosporidium
infection are unsafe water, lack of regular hand washing, and insufficiency of exclusive breastfeeding. This study reports, presumably for the first time, the detection of
Cryptosporidium
oocysts in Chattogram metropolitan city of Bangladesh.