The state of leucocyte adhesiveness/aggregation (LAA) in the peripheral blood of burned mice: an early and sensitive inflammatory indicator and a marker of pulmonary leukostasis

Fried, Marvin P.; Ben-Hur, N; Berliner, Shlomo; Medalia, Ora; Aronson, Moshe; Kidron, Dvora; Ben-Bassat, M

doi:10.1016/0305-4179(91)90071-n

Cited by 17 publications

(11 citation statements)

References 7 publications

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“…In such circumstances, physicians will generally use other tests for acute-phase reactants, including the erythrocyte sedimentation rate or quantitation of C-reactive protein. However, it usually takes more then 24 h to reach a significant increment in the concentration of acute-phase proteins when it is present [35], while the increment in the LAA state is instantaneous [36], a potential advantage.…”

Section: Discussionmentioning

confidence: 99%

The Inflammation Meter: Novel Technology to Detect the Presence of Infection/Inflammation in Patients without Leukocytosis but with Increased Leukocyte Adhesiveness/Aggregation

Maharshak¹,

Kassirer²,

Zeltser³

et al. 2000

Acta Haematol

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Objectives: To reveal the presence of infection/inflammation in patients with relatively normal white blood cell count (WBCC) by using the leukocyte adhesiveness/aggregation test (LAAT). Methods: The LAAT was performed by using a simple slide test and image analysis (Inflamet™), the WBCC, by an electronic cell analyzer, C-reactive protein, by Laser nephelometry and CD11b/CD18 by whole blood flow cytometry. Results: Forty out of a cohort of 121 patients with nonviral acute febrile illness had a WBCC within normal limits. The intensity of the inflammatory response in these individuals as judged by either C-reactive protein, or fibrinogen concentrations, erythrocyte sedimentation or polymorphonuclear leukocyte CD11b/CD18 expression was similar to that observed in patients with a leukocytic response. Our present finding that 63% out of the group with documented infection/inflammation and no leukocytosis had a significantly increased LAAT suggest that the lack of leukocytosis is in part a pseudoleukopenia, or is associated with some degree of uncompensated tissue leukostasis. Conclusions: The lack of a leukocytic response in a patient with nonviral infection/inflammation is by no means a sign of a less inflammatory response. The increased state of leukocyte adhesiveness/aggregation might help to disclose the presence of inflammation in these individuals.

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Section: Discussionmentioning

confidence: 99%

The Inflammation Meter: Novel Technology to Detect the Presence of Infection/Inflammation in Patients without Leukocytosis but with Increased Leukocyte Adhesiveness/Aggregation

Maharshak¹,

Kassirer²,

Zeltser³

et al. 2000

Acta Haematol

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“…In various earlier studies it has been shown to correlate with disease activity during infections [1^3], rheumatic diseases [4^5], ischaemic heart diseases [6^8], brain conditions [9^13], in£ammatory bowel diseases [14^16], as well as trauma-related in£ammatory responses [17,18] and atherosclerosis [19]. This test is based on the observation that white blood cells increase their adhesiveness following in£am-matory stimuli [20].…”

Section: Introductionmentioning

confidence: 99%

The leukocyte adhesiveness/aggregation test as an inflammation-related, plasma-dependent agglutination phenomenon

Zeltser¹,

Kassirer²,

Shapira³

et al. 1998

Scandinavian Journal of Clinical and Laboratory Investigation

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The leukocyte adhesiveness/aggregation test (LAAT) is a non-specific marker of inflammation. In the present study, we examined the expression of the CD11b/CD18 as well as the CD62L antigens on the surface of peripheral blood leukocytes in 84 patients with various inflammatory conditions and 60 controls by using whole blood flow cytometry. We also conducted several in vitro experiments in order to explain the mechanism of the leukocyte adhesiveness/aggregation. A significant (r= -0.36, p<0.001) negative correlation was found between the expression of CD62L on the surface of peripheral blood polymorphonuclears and the state of LAA. There was no correlation between the availability of the CD11b/CD18 antigen and the adhesive state of these cells. In a series of in vitro experiments, we could show that it is possible to significantly reduce the number of aggregated leukocytes in the peripheral blood by using dilutions with buffer but not with plasma. There is no change in the degree of aggregation following incubation at low temperature in the presence of an aerobic metabolism blocker or following incubation with a divalent ion chelator. Additionally, white blood cells could be seen to adhere to protein-rich areas in the peripheral slides. We assume that the state of LAA in the peripheral blood as revealed by the LAA test is more a plasma factor-dependent agglutination than a firm aggregation phenomenon. Factors other than the leukocyte integrins or selectins should be sought as being responsible for this LAA phenomenon.

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“…The LAAT has been previously shown to be a reliable indicator of the existence and severity of the inflammatory reaction accompanying infectious disease [13,15,16,26], sepsis [12], rheumatic disease [2,4], ischemic heart disease [9,17], cerebrovascular disease [7,8,10,27], inflammatory bowel disease [5,6,18], burns [28] and stress [29][30][31][32]. We presently focused on the possibility to identify the WBC and their state of adhesiveness/aggregation in both venous and capillary blood by using an image analysis system.…”

Section: Discussionmentioning

confidence: 99%

“…Yet it takes many hours and even days to obtain significantly elevated concentrations of fibrinogen, globulins and accelerated ESR [44]. In contrast, the appearance of leukocytosis and increased LAA is extremely rapid [28]. This observation is relevant due to the importance of eventual detection of bacterial infections at an early stage.…”

Section: Discussionmentioning

confidence: 99%

Capillary and Venous Leukocyte Adhesiveness/Aggregation to Differentiate between Viral and Bacterial Infection

Rotstein¹,

Kassirer²,

Zeltser³

et al. 2002

Infection

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The state of leucocyte adhesiveness/aggregation (LAA) in the peripheral blood of burned mice: an early and sensitive inflammatory indicator and a marker of pulmonary leukostasis

Cited by 17 publications

References 7 publications

The Inflammation Meter: Novel Technology to Detect the Presence of Infection/Inflammation in Patients without Leukocytosis but with Increased Leukocyte Adhesiveness/Aggregation

The Inflammation Meter: Novel Technology to Detect the Presence of Infection/Inflammation in Patients without Leukocytosis but with Increased Leukocyte Adhesiveness/Aggregation

The leukocyte adhesiveness/aggregation test as an inflammation-related, plasma-dependent agglutination phenomenon

Capillary and Venous Leukocyte Adhesiveness/Aggregation to Differentiate between Viral and Bacterial Infection

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