The stability of the serotypes ofBordetella pertussiswith particular reference to serotype 1,2,3,4

Abstract

SUMMARYStrains ofBordetella pertussisin which all the organisms contain agglutinogens 1 and 3 or 1, 2 and 4 are easy to identify as serotypes 1,0,3,0 and 1,2,0,4 respectively; and similarly, stable strains of serotype 1,0,3,4 are occasionally found. During repeated subcultures, passagein vivo, and lyophilization and preservation for many years, these serotypes do not change. Mixing 1,0,3,0 and 1,2,0,4 serotypes and culturing them togetherin vivoandin vitroproduces cultures from which organisms of the same two serotypes can be isolated.In contrast, strains which type as 1,2,3,4 are often a heterogeneous group. We have attempted to classify these as 'stable', 'variable' and 'mixed' cultures. Some strains comprise organisms all of which contain the four agglutinogens and are as easy to type as the strains described above. These we have called 'stable' 1,2,3,4 strains. Other 1,2,3,4 strains are made up of colonies possessing all four agglutinogens, as shown by agglutinin production, but in amounts varying from day to day so that direct typing is inconsistent. These we have called 'variable' 1,2,3,4 strains. The last category, 'mixed', is made up of organisms most of which give rise to stable 1,2,3,4 cultures; a few of the component organisms, however, have one or two of the four agglutinogens missing. The importance of the 'variable' cultures is emphasized for work on apparent change of serotype, e.g. during infection.

Genetic diversity and relationships in populations of Bordetella spp

“…Serotype variation in strains of B. pertussis also occurs spontaneously in vitro and in vivo (6). This propensity for phenotypic modification has limited the conclusions that can be drawn from epidemiological studies in which serologic typing techniques are used and thus has hampered research in this area.…”

Rabbit nasopharyngeal colonization byBordetella pertussis: the effects of immunization on clearance and on serum and nasal antibody levels

“…Strains were obtained from the following sources: strain M2 (1, 3 serotype) from Dr N. Preston, Department of Bacteriology, University of Manchester; strain Tohama (1,2) from Dr C. R. Manclark, Bureau of Biologics, Food and Drug Administration, Bethesda, Maryland: strain B106 (1, 3) from the Lister Institute (Bronne-Shanbury & Dolby, 1976); strain wellcome 28 (1,2,3) from Dr P. Novotny, Wellcome Research Laboratories, Beckenham. All strains were maintained as freeze-dried stocks and recovered by growth on charcoal agar (Oxoid) containing 100 defibrinated horse blood and 0-2 u./ml penicillin.…”

The biofilm formation of freshly isolated and vaccine strains of Bordetella pertussis of different serotypes

“…Агглютиногены 1, 2 и 3 используются для идентификации разных штаммов и принадлежности их к определенному серовару. Сочетания этих агглютиногенов определяют три основных серовара возбудителя: 1.2.0, 1.2.3 и 1.0.3 [6,8]. Микробные клетки B. pertussis имеют фимбрии, состоящие из двух основных субъединиц -Fim 2 и Fim3, соответствующих агглютиногенам 2 и 3, и малой субъединицы Fim D. Наряду с филаментозным гемагглютинином и другими адгезинами фимбрии принимают участие в процессе адгезии микробных клеток на субстрате [7].…”