Genetic diversity and relationships in populations of Bordetella spp

Abstract

Genetic diversity in 60 strains of three nominal Bordetella species recovered from humans and other mammalian hosts was assessed by analyzing electrophoretically demonstrable allelic variation at structural genes encoding 15 enzymes. Eleven of the loci were polymorphic, and 14 distinctive electrophoretic types, representing multilocus genotypes, were identified. The Approximately one-half of the strains were received and analyzed under code. Many of the strains have been described previously, and some have been passaged in vitro more than 100 times (3,4,15,27).Growth of bacteria and electrophoresis of enzymes. The isolates were grown on Bordet-Gengou medium (Remel, Lenexa, Kans.) in a humidified atmosphere containing 5% CO2 and were examined visually for purity. Each isolate was then grown in 400 ml of modified Stanier-Scholte medium

Isolation and characterization of Bordetella parapertussis-like bacteria from ovine lungs

“…In general, most of the isolates showed biochemical reactions which were similar to those described for B. parapertzlssis (Pittman, 1984). The main characteristic biochemical property was the ability of the isolates to causing browning of tyrosine agar, a characteristic unique to B. parapertusxix (Ensminger, 1953 …”

“…This organism is more usually considered to be an obligate respiratory tract pathogen of man (Linneman & Perry, 1977;Pittman, 1984). In all, 33 Bordetella isolates were obtained from the lungs of 52 young sheep.…”

“…Its fastidiousness and defined growth requirements (Proom, 1955) make it difficult to isolate without specialized media (Regan & Lowe, 1977) and identification is hampered by the close similarity of members of the genus Bordetella with other bacterial genera, in particular Haemopbilzrs, Acinetohacter and Alcaligenes (Johnson & Sneath, 1973). Considered an obligate respiratory tract pathogen for man (Linneman & Perry, 1977 ;Pittman, 1984) it has recently been isolated from the respiratory tract of healthy and pneumonic lambs in New Zealand (Cullinane e t al., 1987). Studies in mice (Chen e t al., 1989) and sheep (Chen e t al., 1988a, b) have demonstrated its pathogenicity for both species.…”

Sequence variation and conservation in virulence-related genes of Bordetella pertussis isolates from the UK

“…In the present study, sequence variation was determined in regions of ten virulence-associated genes (prnA, ptxA, ptxC, tcfA, fim2, brkA, vag8, ompQ, bapC and cyaA) in a selection of historical and recent UK isolates of B. pertussis, together with the three strains used to produce the UK whole-cell pertussis vaccine, the strain used to produce components for the acellular pertussis vaccine (Tohama-I) and the type strain (18323 T ) of B. pertussis (Tables 3 and 4). The vaccine and type strains were included as reference strains to help identify vaccine and non-vaccine allele types and to increase the probability of identifying polymorphic sites, as the type strain has been shown to be genotypically distinct from other B. pertussis strains (Musser et al, 1986;Gerlach et al, 2001). In B. pertussis isolates from the UK for the period 1920-2002, polymorphism was found in five genes, prnA, ptxA, ptxC, tcfA and fim2.…”

pagPIs Required for Resistance to Antibody-Mediated Complement Lysis duringBordetella bronchisepticaRespiratory Infection

“…The genus Bordetella presently consists of eight species, some of which are known to cause respiratory tract infections in a variety of animals and humans (14). Bordetella bronchiseptica infection results in respiratory disease in pigs, dogs, rabbits, rats, mice, and other nonhuman mammals, while the closely related species B. pertussis is the causative agent of whooping cough in humans (8,10).…”