The Stability of Chromosomes in Yeast

Larionov, Vladimir; Karpova, Tatiana; Zhouravleva, Galina A.; Pashina, O. B.; Nikolaishvili, N. T.; Kouprina, Natalya

doi:10.1007/978-3-642-46611-3_1

Cited by 7 publications

(6 citation statements)

References 24 publications

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“…Also, loss of one copy of chromosome IIZ will lead to hemizygotization of MAT and leu2 alleles (MATa leu2-27 or MATa leu2-1). Loss of chromosome ZZZ in diploid strains homozygous for ch14 was also recorded in this way (LARIONOV et al 1987(LARIONOV et al , 1989.…”

Section: Determination Of Rates and Frequencies Of Recombination And Chromosome Zzz Loss By Fluctuation Analysismentioning

confidence: 74%

“…Genotvw Source for Y2CpI and inverted for Y2Cp2). The CpBl plasmid is a derivative of the YCp41 plasmid in which a 0.6-kb fragment containing a CALI promoter was inserted at a unique BamHl site of YCp41 plasmid (LARIONOV et al 1987). pAS58 plasmid was obtained from A. STRUNNIKOV.…”

Section: Strainmentioning

confidence: 99%

“…Introduction of a second centromere into chromosome Z Z k For creation of dicentric chromosome I l l , the plasmid CpBl (CALl:CEN3/LEU2/ARSl) was used (LARIONOV et al 1987). This plasmid carrying a conditional centromere was cleaved at the KpnI site (localized in the LEU2 gene) and was used for integrative transformation of the yeast strains YNK45 (ch14) and YNK42 (wild type).…”

Section: Strainmentioning

confidence: 99%

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Identification and cloning of the CHL4 gene controlling chromosome segregation in yeast.

et al. 1993

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A collection of chl mutants characterized by decreased fidelity of chromosome transmission and by minichromosome nondisjunction in mitosis was examined for the ability to maintain nonessential dicentric plasmids. In one of the seven mutants analyzed, chl4, dicentric plasmids did not depress cell division. Moreover, nonessential dicentric plasmids were maintained stably without any rearrangements during many generations in the chl4 mutant. The rate of mitotic heteroallelic recombination in the chl4 mutant was not increased compared to that in an isogenic wild-type strain. Analysis of the segregation of a marked chromosome indicated that sister chromatid nondisjunction and sister chromatid loss contributed equally to chromosome malsegregation in the chl4 mutant. A genomic clone of CHL4 was isolated by complementation of the chl4-1 mutation and was physically mapped to the right arm of chromosome IV near the SUP2 gene. Nucleotide sequence analysis of CHL4 clone revealed a 1.4-kb open reading frame coding for a 53-kD predicted protein which does not have homology to published proteins. A strain containing a null allele of CHL4 is viable under standard growth conditions but has a temperature-sensitive phenotype (conditional lethality at 36 degrees). We suggest that the CHL4 gene is required for kinetochore function in the yeast Saccharomyces cerevisiae.

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Section: Determination Of Rates and Frequencies Of Recombination And Chromosome Zzz Loss By Fluctuation Analysismentioning

confidence: 74%

Section: Strainmentioning

confidence: 99%

Section: Strainmentioning

confidence: 99%

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Identification and cloning of the CHL4 gene controlling chromosome segregation in yeast.

et al. 1993

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“…Assays of recombination, spontaneous mutation and chromosome loss rates and frequencies: The frequency of chromosome ZZZ loss in disomic cells was determined by measuring concurrent hemizygotization of three markers of chromosome ZZZ: MAT, his4 and thr4. Loss of chromosome ZZZ in diploid strains homozygous for chll2 was also recorded in this way (LARIoNov et al 1987).…”

mentioning

confidence: 66%

“…The frequency of spontaneous mitotic recombination in the ch112 mutants was determined by characterizing the missegregation of chromosome ZZZmarkers (his4 thr4/HZS4 THR4) (LARIoNov et al 1987).…”

mentioning

confidence: 99%

CHL12, a gene essential for the fidelity of chromosome transmission in the yeast Saccharomyces cerevisiae.

et al. 1994

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We have analyzed the CHL12 gene, earlier identified in a screen for yeast mutants with increased rates of mitotic loss of chromosome III and circular centromeric plasmids. A genomic clone of CHL12 was isolated and used to map its physical position on the right arm of chromosome XIII near the ADH3 locus. Nucleotide sequence analysis of CHL12 revealed a 2.2-kb open reading frame with a 84-kD predicted protein sequence. Analysis of the sequence upstream of the CHL12 open reading frame revealed the presence of two imperfect copies of MluI motif, ACGCGT, a sequence associated with many DNA metabolism genes in yeast. Analysis of the amino acid sequence revealed that the protein contains a NTP-binding domain and shares a low degree of homology with subunits of replication factor C (RF-C). A strain containing a null allele of CHL12 was viable under standard growth conditions, and as well as original mutants exhibited an increase in the level of spontaneous mitotic recombination, slow growth and cold-sensitive phenotypes. Most of cells carrying the null chl12 mutation arrested as large budded cells with the nucleus in the neck at nonpermissive temperature that typical for cell division cycle (cdc) mutants that arrest in the cell cycle at a point either immediately preceding M phase or during S phase. Cell cycle arrest of the chl12 mutant requires the RAD9 gene. We conclude that the CHL12 gene product has critical role in DNA metabolism.

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Mitotic hyperploidy for chromosomes VIII and III in Saccharomyces cerevisiae

Spector

Fogel

1992

Curr Genet

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The arg4-8 and cup1s markers comprise a copy-number-dependent signal device in the yeast Saccharomyces cerevisiae. These alleles permit reliable discrimination between euploid and disomic haploids as well as between euploid and trisomic diploids. To investigate and compare inherent inter-chromosomal differences as regards propensity for hyperploidy, we transplaced arg4-8 and cup1s by deleting them from chromosome VIII and then re-introducing them at the leu2 locus on chromosome III. The rate of chromosome gain was significantly greater for the chromosome III construct compared to the native chromosome VIII, in both diploid and haploid strains. In addition, more coincident aneuploidy for other chromosomes was found among chromosome VIII hyperploids compared to chromosome III hyperploids.

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The Stability of Chromosomes in Yeast

Cited by 7 publications

References 24 publications

Identification and cloning of the CHL4 gene controlling chromosome segregation in yeast.

Identification and cloning of the CHL4 gene controlling chromosome segregation in yeast.

CHL12, a gene essential for the fidelity of chromosome transmission in the yeast Saccharomyces cerevisiae.

Mitotic hyperploidy for chromosomes VIII and III in Saccharomyces cerevisiae

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