Mitotic hyperploidy for chromosomes VIII and III in Saccharomyces cerevisiae

Spector, Lorraine M.; Fogel, Seymour

doi:10.1007/bf00351688

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…Diploids used to detect spores disomic for chromosome III (BR4316, BR4310, and BR4633) are homozygous for insertion of a single CUP1 gene and an arg4-8 ts gene at the LEU2 locus on chromosome III (Spector and Fogel 1992). Both genes are deleted from their normal locations on chromosome VIII.…”

Section: Methodsmentioning

confidence: 99%

Centromere-Proximal Crossovers Are Associated With Precocious Separation of Sister Chromatids During Meiosis inSaccharomyces cerevisiae

2006

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In most organisms, meiotic chromosome segregation is dependent on crossovers (COs), which enable pairs of homologous chromosomes to segregate to opposite poles at meiosis I. In mammals, the majority of meiotic chromosome segregation errors result from a lack of COs between homologs. Observations in Homo sapiens and Drosophila melanogaster have revealed a second class of exceptional events in which a CO occurred near the centromere of the missegregated chromosome. We show that in wild-type strains of Saccharomyces cerevisiae, most spore inviability is due to precocious separation of sister chromatids (PSSC) and that PSSC is often associated with centromere-proximal crossing over. COs, as opposed to nonreciprocal recombination events (NCOs), are preferentially associated with missegregation. Strains mutant for the RecQ homolog, SGS1, display reduced spore viability and increased crossing over. Much of the spore inviability in sgs1 results from PSSC, and these events are often associated with centromere-proximal COs, just as in wild type. When crossing over in sgs1 is reduced by the introduction of a nonnull allele of SPO11, spore viability is improved, suggesting that the increased PSSC is due to increased crossing over. We present a model for PSSC in which a centromere-proximal CO promotes local loss of sister-chromatid cohesion.

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Section: Methodsmentioning

confidence: 99%

Centromere-Proximal Crossovers Are Associated With Precocious Separation of Sister Chromatids During Meiosis inSaccharomyces cerevisiae

2006

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“…These markers were acquired in crosses to strain 6D (Spector and Fogel 1992). Both arg4-8 and CUP1 confer dosage-dependent phenotypes (Rockmill and Fogel 1988).…”

Section: Yeast Strainsmentioning

confidence: 99%

Telomere-mediated chromosome pairing during meiosis in budding yeast

Rockmill¹,

Roeder²

1998

Genes Dev.

122

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Certain haploid strains of Saccharomyces cerevisiae can undergo meiosis, but meiotic prophase progression and subsequent nuclear division are delayed if these haploids carry an extra chromosome (i.e., are disomic). Observations indicate that interactions between homologous chromosomes cause a delay in meiotic prophase, perhaps to allow time for interhomolog interactions to be completed. Analysis of meiotic mutants demonstrates that the relevant aspect of homolog recognition is independent of meiotic recombination and synaptonemal complex formation. A disome in which the extra chromosome is circular sporulates without a delay, indicating that telomeres are important for homolog recognition. Consistent with this hypothesis, fluorescent in situ hybridization demonstrates that a circular chromosome has a reduced capacity to pair with its homolog, and a telomere-associated meiotic protein (Ndj1) is required to delay sporulation in disomes. A circular dimer containing two copies of the same chromosome delays meiosis to the same extent as two linear homologs, implying that physical proximity bypasses the requirement for telomeres in homolog pairing. Analysis of a disome carrying two linear permuted chromosomes suggests that even nonhomologous chromosome ends can promote homolog pairing to a limited extent. We speculate that telomere-mediated chromosome movement and/or telomere clustering promote homolog pairing.

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“…Earlier attempts to characterize gene duplication were mainly based on the yeast CUP1 gene dosage selection system, and different mechanisms of primary gene amplification, such as aneuploidization, extrachromosomal amplification or cycle of breakage–fusion–bridge, were described (Whittaker et al , 1988; Spector and Fogel, 1992; Dorsey et al , 1993; Moore et al , 2000). Some DNA content variations of large chromosomal regions were reported as potential large segmental duplications in the Saccharomyces cerevisiae genome (Bach et al , 1995; Roelants et al , 1995; Hughes et al , 2000).…”

Section: Introductionmentioning

confidence: 99%

Eucaryotic genome evolution through the spontaneous duplication of large chromosomal segments

Koszul

Caburet

Dujon

et al. 2003

EMBO J

193

197

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There is growing evidence that duplications have played a major role in eucaryotic genome evolution. Sequencing data revealed the presence of large duplicated regions in the genomes of many eucaryotic organisms, and comparative studies have suggested that duplication of large DNA segments has been a continuing process during evolution. However, little experimental data have been produced regarding this issue. Using a gene dosage assay for growth recovery in Saccharomyces cerevisiae, we demonstrate that a majority of the revertant strains (58%) resulted from the spontaneous duplication of large DNA segments, either intra-or interchromosomally, ranging from 41 to 655 kb in size. These events result in the concomitant duplication of dozens of genes and in some cases in the formation of chimeric open reading frames at the junction of the duplicated blocks. The types of sequences at the breakpoints as well as their superposition with the replication map suggest that spontaneous large segmental duplications result from replication accidents. Aneuploidization events or suppressor mutations that do not involve largescale rearrangements accounted for the rest of the reversion events (in 26 and 16% of the strains, respectively).

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Mitotic hyperploidy for chromosomes VIII and III in Saccharomyces cerevisiae

Cited by 5 publications

References 33 publications

Centromere-Proximal Crossovers Are Associated With Precocious Separation of Sister Chromatids During Meiosis inSaccharomyces cerevisiae

Centromere-Proximal Crossovers Are Associated With Precocious Separation of Sister Chromatids During Meiosis inSaccharomyces cerevisiae

Telomere-mediated chromosome pairing during meiosis in budding yeast

Eucaryotic genome evolution through the spontaneous duplication of large chromosomal segments

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