The soybean lipoxygenase-fluorescein reaction may be used to assess antioxidant capacity of phytochemicals and serum

Soccio, Mario; Laus, Maura N.; Alfarano, Michela; Pastore, Donato

doi:10.1039/c6ay01002d

Cited by 14 publications

(43 citation statements)

References 32 publications

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“…TAC assessment is an established methodology for measuring the overall antioxidant defence system; in recent years, a variety of assays with different mechanisms have been developed to monitor TAC [7][8][9]. Very recently, a novel approach has been developed, which is based on the determination of Antioxidant/Oxidant Balance, representing the ratio between serum antioxidant capacity and serum oxidant status [10].An increasing number of studies focus on the role of ROS in the pathogenesis of numerous diseases of civilisation, such as obesity [11].…”

Section: Total Serum Antioxidant Capacity In Healthy Normal Weight Anmentioning

confidence: 99%

Total Serum Antioxidant Capacity in Healthy Normal Weight and Asymptomatic Overweight Adults

Petelin

Tedeschi

Maietti

et al. 2017

Exp Clin Endocrinol Diabetes

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Obesity and overweight are major contributors to the burden of chronic disease. Both are defined as abnormal or excessive fat accumulation and by increased production of free radicals leading to oxidative stress. The aim of the present study was to evaluate whether overweight and fat accumulation is associated with serum total antioxidant capacity (TAC) in men and women, irrespective of nutritional habits, nutrient intakes, physical activity, smoking, and other confounders, which may be responsible for modifying the association between serum TAC and overweight/obesity measures. This cross-sectional study was conducted on 60 normal weight and 60 overweight adults aged 25-49. All participants underwent standard anthromorphological measurements of body composition, blood pressure and biochemical measurements, aerobic capabilities assessment and dietary intake evaluation. TAC was measured by using the photochemioluminescence method. All data were analysed with SPSS software. Men had higher values of TAC than women and concentrations of TAC were significantly higher in overweight subjects compared to normal weight subjects. In the present study TAC tended to be increased by various metabolic risk factors, especially overweight/obesity parameters (body mass index, body fat), inflammation and increased serum levels of Cysteine, irrespective of nutritional habits, nutrient intakes, physical activity and smoking. Overweight and obesity at an early stage may stimulate TAC. Therefore, the elevation of TAC in overweight adults may be a compensatory response to oxidative stress, generated by reactive oxygen species.

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Section: Total Serum Antioxidant Capacity In Healthy Normal Weight Anmentioning

confidence: 99%

Total Serum Antioxidant Capacity in Healthy Normal Weight and Asymptomatic Overweight Adults

Petelin

Tedeschi

Maietti

et al. 2017

Exp Clin Endocrinol Diabetes

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“…The LOX-FL reaction was performed as recently described in [5], modified as in [1]. The quenching of 3׳,6׳-dihydroxy-3H-spiro[2-benzofuran-1,9׳-xanthen]-3-one (fluorescein, FL) coupled to the LOX-1-dependent linoleate peroxidation was continuously monitored ( λ ex =485 nm, λ em =515 nm) at 37 °C using a LS 55 fluorescence spectrometer (Perkin Elmer, Waltham, MA, USA).…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…The ORAC measurements were performed according to the protocol reported in [3], properly modified as in [1], [5]. The FL fluorescence decay due to peroxyl radicals generated by 2,2’-azobis(2-methylpropionamidine) dihydrochloride (AAPH) thermal decomposition was monitored ( λ ex =483 nm, bandwidth 14 nm; λ e m =530 nm, bandwidth 30 nm) at 37 °C every 30 s by means of a CLARIOstar microplate reader (BMG Labtech, Ortenberg, Germany).…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…AC values were obtained by using three different assays: two widely used protocols, such as Trolox Equivalent Antioxidant Capacity (TEAC) [2] and Oxygen Radical Absorbance Capacity (ORAC) [3] methods, and the recently developed Lipoxygenase-Fluorescein (LOX-FL) method [4], derived from the LOX/4-nitroso- N,N -dimethylaniline (LOX/RNO) one [5]. PxL values were obtained as described in [1].…”

Section: Datamentioning

confidence: 99%

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Serum antioxidant capacity and peroxide level of seven healthy subjects after consumption of different foods

et al. 2016

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This article reports experimental data related to the research article entitled “Different effectiveness of two pastas supplemented with either lipophilic or hydrophilic/phenolic antioxidants in affecting serum as evaluated by the novel Antioxidant/Oxidant Balance approach” (M.N. Laus, M. Soccio, M. Alfarano, A. Pasqualone, M.S. Lenucci, G. Di Miceli, D. Pastore, 2016) [1]. Antioxidant status of blood serum of seven healthy subjects was evaluated during four hours after consumption of two functional pastas, supplemented with either bran oleoresin or bran water extract obtained from durum wheat. For comparison, the effect of a non-supplemented reference pasta was also evaluated, as well as the effects of glucose, of the wheat grain dietary supplement Lisosan G, and of the reference pasta consumed together with Lisosan G. Serum antioxidant status was evaluated by measuring both the serum antioxidant capacity, using LOX-FL, ORAC and TEAC methods, and the serum oxidant status, assessed as peroxide level.

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“…ORAC assay was performed as reported in Ou et al (2001), modified as described in Soccio et al (2016), by using a CLARIOstar microplate reader (BMG Labtech, Ortenberg, Germany) and 96-well plates. The TEAC protocol described in Re et al (1999) and modified as in Laus et al (2013Laus et al ( , 2015 was applied.…”

Section: Determination Of Antioxidant Capacity By Means Of Oxygen Radmentioning

confidence: 99%

Antioxidant capacity, phenolic and vitamin C contents of quinoa (Chenopodium quinoa Willd.) as affected by sprouting and storage conditions

et al. 2017

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Antioxidant capacity (AC) of quinoa (Chenopodium quinoa Willd. cv. Real) seeds and sprouts obtained after 4 days of seed germination at 20°C and 70% humidity was evaluated using trolox equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC) assays, able to highlight reducing activity and peroxyl radical scavenging capacity, respectively; phenolic content (PC) was also measured. Both TEAC and ORAC assays revealed a significantly higher (about 2-and 2.8-fold, respectively) AC of 4-day-old sprouts compared to seeds; consistently, also PC values of sprouts resulted about 2.6 times higher than seeds. In order to investigate the influence of storage on AC and PC, as well as on vitamin C content (VCC), 4-day-old sprouts were subjected for 7 days at 5°C to three different conditions of controlled atmosphere storage (CAS) compared with air. Interestingly, whatever the CAS conditions, storage of quinoa sprouts up to 7 days induced an increase of AC evaluated in terms of reducing activity by TEAC assay. Consistently, an increase of PC and VCC was measured during storage, positively correlated to TEAC values. Moreover, a decrease of peroxyl radical scavenging activity, measured by ORAC, was observed after 7 days of storage, in accordance with a shift of AC towards the reducing activity component. Overall, these findings indicate that sprouting approach using quinoa may provide highly antioxidant-enriched seedlings that may improve nutritional quality of diet or of functional foods. Interestingly, antioxidant properties of quinoa sprouts may be deeply influenced by storage, able to increase reducing activity by increasing phenols and vitamin C.

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The soybean lipoxygenase-fluorescein reaction may be used to assess antioxidant capacity of phytochemicals and serum

Abstract: The new LOX-FL method highlights improvement of serum antioxidant capacity after antioxidant intake better than widely used methods.

Cited by 14 publications

References 32 publications

Total Serum Antioxidant Capacity in Healthy Normal Weight and Asymptomatic Overweight Adults

Total Serum Antioxidant Capacity in Healthy Normal Weight and Asymptomatic Overweight Adults

Serum antioxidant capacity and peroxide level of seven healthy subjects after consumption of different foods

Antioxidant capacity, phenolic and vitamin C contents of quinoa (Chenopodium quinoa Willd.) as affected by sprouting and storage conditions

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