The sow endometrium at different stages of the oestrous cycle: studies on morphological changes and infiltration by cells of the immune system

Kaeoket, Kampon; Persson, Erik; Dalin, Anne-Marie

doi:10.1016/s0378-4320(00)00211-6

Cited by 80 publications

(113 citation statements)

References 40 publications

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“…This suggests that interferons could also play a role in endometrial gene regulation during the oestrous cycle or the corresponding genes are additionally regulated through P 4 effects. However, in a study of porcine endometrium, lymphocytes and other immune cells have been shown to immigrate into the endometrium during dioestrus (Kaeoket et al 2001). In the interaction network of the luteal stage of the oestrous cycle, two of the most connective nodes are MAP kinase 1 (MAPK1; cluster 1) and P 4 .…”

Section: Discussionmentioning

confidence: 99%

Dynamic changes in messenger RNA profiles of bovine endometrium during the oestrous cycle

Mitko¹,

Ulbrich²,

Wenigerkind³

et al. 2008

Reproduction

114

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During the oestrous cycle, the bovine endometrium exhibits characteristic morphological and functional changes, which are mainly induced by progesterone (P 4 ), oestrogens and oxytocin. We studied the response of the endometrium to this changing hormonal environment at the transcriptome level using a custom-made cDNA microarray. Endometrium samples were recovered from Simmental heifers on days 0 (oestrus), 3.5 (metoestrus), 12 (dioestrus) and 18. The latter group was divided into animals with high (late dioestrus) and low P 4 levels (preoestrus). Significance analysis of microarrays revealed 269 genes exhibiting significant changes in their transcript levels during the oestrous cycle in distinct temporal patterns. Two major types of expression profiles were observed, which showed the highest mRNA levels during the oestrus phase or the highest levels during the luteal phase respectively. A minor group of genes exhibited the highest mRNA levels on day 3.5. Gene ontology (GO) analyses revealed GO categories related to extracellular matrix remodelling, transport, and cell growth and morphogenesis enriched at oestrus, whereas immune response and particular metabolic pathways were overrepresented at dioestrus.

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Section: Discussionmentioning

confidence: 99%

Dynamic changes in messenger RNA profiles of bovine endometrium during the oestrous cycle

Mitko¹,

Ulbrich²,

Wenigerkind³

et al. 2008

Reproduction

114

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“…The endometrial epithelium cells respond to hormones and in this way play a significant role in endometrial physiology and pathology to [8]. The endocrinological abnormalities in cystic ovarian disease may have complex effects on the endometrium which is a steroid hormone-dependent tissue whose cellular components and tissue growth and remodeling respond to changes in circulating hormones [3].…”

Section: Discussionmentioning

confidence: 99%

Histological changes within ovarian cortex, oviductal and uterine mucosa in case of ovarian cysts presence in sows.

Szulańczyk

2009

Folia Histochem Cytobiol.

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Abstract:The aim of study was histological evaluation of oviduct and uterus mucosa in case of cystical ovarian degeneration in sows. Materials for evaluations were received after slaughtering of 294 sows at age of 2-5 years. Sows were eliminated breeding and culled due to reproduction disorders: anoestrus after weaning of piglets, return of estrus, small litters and due to age, number of birth, bad condition after lactation. 20 sows (6.8%) had cysts on ovaries. Histological investigations were conducted on reproductive organs of sows with ovarian cysts. Sows were divided onto two groups: 1. with polycystic ovaries (12 sows), 2. with simple cyst (8 sows) occured unilaterally (3 sows) or bilaterally (5 sows). In our studies we noted different kids of ovarian cysts and structural changes in ovarian cortex like decreasing numbers of ovarian folliculi of all generations, increasing of follicular atresia. We compared the state of oviduct and uterus mucosa in case of polycystic ovaries with the histological changes in these tissues when single cysts were present on the ovaries. The single follicular cysts aren't accompanied with important changes in reproductive system. In case of polycystic ovaries the presence of cyst is connected with occurrence of morphological changes in endothelium of oviduct and uterus which would have been a reason of persistent infertility of pigs.

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“…The sows were randomly assigned to 3 groups, i.e., CAI (n=5), IUI (n=5) and DIUI (n=5). Transrectal ultrasonography (Echo camera SSD-550, Aloka Co. Ltd., Japan) was performed every 4 hr using a 5 MHz probe to examine the ovulation time in all sows [7,20].…”

Section: Animals and The Detection Of Oestrus And Ovulationmentioning

confidence: 99%

“…It is not known yet whether these two transcervical insemination techniques will cause positive or negative effect on the immune function of the sow's reproductive tracts. Earlier studies have demonstrated that lymphocytes are the dominant immune cells in the gilts' [18] and sows' endometrium [7] and most of these lymphocytes were T cells [6]. Furthermore, neutrophils and macrophages are observed in all tissue layers of the gilts/sows' endometrium, especially during the follicular phase [7,18] and endometritis [19].…”

mentioning

confidence: 99%

“…Earlier studies have demonstrated that lymphocytes are the dominant immune cells in the gilts' [18] and sows' endometrium [7] and most of these lymphocytes were T cells [6]. Furthermore, neutrophils and macrophages are observed in all tissue layers of the gilts/sows' endometrium, especially during the follicular phase [7,18] and endometritis [19]. After AI, the numbers of neutrophils in the sows' endometrium is also increased [8].…”

mentioning

confidence: 99%

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Infiltration of Local Immune Cells in the Sow Reproductive Tracts after Intra-Uterine and Deep Intra-Uterine Insemination with a Reduced Number of Spermatozoa is Less than Conventional Artificial Insemination

Tummaruk

Tienthai

2011

The Journal of Veterinary Medical Science

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ABSTRACT. The present study investigated the infiltration of leukocyte subpopulations in the utero-tubal junction (UTJ) and each part of the oviducts at about 24 hr after intra-uterine insemination (IUI) and deep intra-uterine insemination (DIUI) compared to conventional artificial insemination (CAI) in sows. Fifteen crossbred Landrace x Yorkshire multiparous sows were used (CAI, n=5; IUI, n=5; DIUI, n=5). The sperm dose contained 3,000  10 6 (100 ml), 1,000  10 6 (50 ml) and 150  10 6 (5 ml) motile spermatozoa for CAI, IUI and DIUI, respectively. The sows were inseminated with extended fresh semen at 6 to 8 hr prior to the expected time of ovulation. At 25.2  1.6 hr after insemination, the oviducts and the UTJ were collected. The tissue samples of UTJ, caudal isthmus, cranial isthmus and ampulla were transversely cut to a thickness of 5 m and stained with H&E. The total numbers of lymphocytes, neutrophils, macrophages, eosinophils and plasma cells were determined under light microscope. It was found that the numbers of lymphocytes, eosinophils and macrophages after CAI, IUI and DIUI were not significantly different (P>0.1) in both epithelial and sub-epithelial connective tissue layer of the UTJ, caudal isthmus, cranial isthmus and ampulla. Intra-epithelial neutrophils in the UTJ were higher than cranial isthmus (P<0.05) and ampulla (P<0.05). In the UTJ, the intra-epithelial neutrophil in the CAI group was higher than DIUI group (P<0.01). Plasma cells in sub-epithelial layer of the endosalpinx in the CAI group were higher than DIUI group (P<0.05) and tended to be higher than the IUI group (P=0.08). In conclusion, compared to CAI, IUI and DIUI do not influence the infiltration of lymphocytes, macrophages and eosinophils in the UTJ and the oviduct prior to fertilization. But a lower number of neutrophils in the intra-epithelial layer of the UTJ and plasma cells in the sub-epithelial layers of the oviduct was observed in the DIUI group compared to CAI.KEY WORDS: immune cell, intra-uterine insemination, oviduct, swine.

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The sow endometrium at different stages of the oestrous cycle: studies on morphological changes and infiltration by cells of the immune system

Cited by 80 publications

References 40 publications

Dynamic changes in messenger RNA profiles of bovine endometrium during the oestrous cycle

Dynamic changes in messenger RNA profiles of bovine endometrium during the oestrous cycle

Histological changes within ovarian cortex, oviductal and uterine mucosa in case of ovarian cysts presence in sows.

Infiltration of Local Immune Cells in the Sow Reproductive Tracts after Intra-Uterine and Deep Intra-Uterine Insemination with a Reduced Number of Spermatozoa is Less than Conventional Artificial Insemination

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