Dynamic changes in messenger RNA profiles of bovine endometrium during the oestrous cycle

Mitko, K.; Ulbrich, Susanne E.; Wenigerkind, Hendrik; Sinowatz, Fred; Blum, Helmut; Wolf, Eckhard; Bauersachs, Stefan

doi:10.1530/rep-07-0415

Cited by 114 publications

(96 citation statements)

References 71 publications

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“…A twin pregnancy was diagnosed if 2 embryonic discs were detected. Endometrial tissue samples were collected, preserved, and processed for isolation of RNA as previously described (38).…”

Section: Methodsmentioning

confidence: 99%

The endometrium responds differently to cloned versus fertilized embryos

Bauersachs

Ulbrich

Zakhartchenko³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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187

144

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Although somatic cell nuclear transfer (SCNT) cloning is more efficient in cattle than in any other species tested so far, there is a high rate of pregnancy failure that has been linked to structural and functional abnormalities of the placenta. We tested the hypothesis that these changes may originate from disturbed embryo-maternal interactions in the peri-implantation period. Therefore, we evaluated the response of the endometrium to SCNT embryos (produced from 7 different fetal fibroblast cell lines) as compared with embryos derived from in vitro fertilization (IVF). SCNT embryos and IVF embryos were cultured under identical conditions to the blastocyst stage (day 7) and were transferred to corresponding recipients, which were slaughtered at day 18 of pregnancy. The mRNA profiles of endometrium samples were obtained using a custom cDNA microarray enriched for transcripts differentially expressed in the endometrium and/or oviduct epithelium during the estrous cycle and/or early pregnancy. Overall, the variation in mRNA profiles was greater in the SCNT group than in the IVF group. Furthermore, 58 transcripts were differentially abundant in endometria from SCNT and IVF pregnancies. Prominent examples are orphan nuclear receptor COUP-TFII and connexin 43, both known to play important roles in uterine receptivity and conceptus placentation. These findings suggest that placental failure in bovine clone pregnancies may originate from abnormal embryomaternal communication that develops during the peri-implantation period. Endometrium transcriptome profiles may serve as a tool to evaluate SCNT embryos for their ability to establish pregnancy and develop a functional placenta.Bos taurus ͉ cloning ͉ early pregnancy ͉ gene expression ͉ microarray C loning by somatic cell nuclear transfer (SCNT) (1) is an important strategic tool for animal breeding and biotechnology. For example, animals carrying rare desired alleles can be multiplied to introduce the desired allele into the breeding population. The life span and capacity of valuable breeding animals and the physiologically limited number of offspring of female animals can be increased, thus enhancing selection intensity. Moreover, cloning allows the propagation of desired genotypes without the risk of genetic recombination that is inherent to sexual reproduction. In animal biotechnology, SCNT using genetically modified donor cells is a powerful approach for the generation of transgenic animals (2) and so far is the only technique facilitating targeted mutagenesis in livestock species (3). Moreover, cloning can be used to multiply transgenic animals and to propagate multitransgenic individuals without segregation of the individual transgenes.Despite the plethora of important applications of cloning, the efficiency of this technology still is very low. Although SCNT is more efficient in cattle than in any other species tested so far, a recent survey covering the results of bovine cloning in Brazil, Argentina, and the United States over a 5-year period revealed that only 9...

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“…A twin pregnancy was diagnosed if 2 embryonic discs were detected. Endometrial tissue samples were collected, preserved, and processed for isolation of RNA as previously described (38).…”

Section: Methodsmentioning

confidence: 99%

The endometrium responds differently to cloned versus fertilized embryos

Bauersachs

Ulbrich

Zakhartchenko³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

187

144

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“…A variety of studies in cattle, including studies from our own group (Forde et al, 2009 and, evaluated genes expressed in the endometrium of the uterus during selected stages of the oestrous cycle and pregnancy (Bauersachs et al, 2005(Bauersachs et al, , 2006Mitko et al, 2008;MansouriAttia et al, 2009). However, data on gene expression during the post-hatching embryonic stages in cattle are sparse and, apart from a few exceptions, typically deal with candidate genes rather than being global in nature (Ushizawa et al, 2004;Hue et al, 2007;Degrelle et al, 2011).…”

Section: Gene Expression In the Conceptusmentioning

confidence: 99%

Maternal-embryo interaction leading up to the initiation of implantation of pregnancy in cattle

Lonergan¹,

Forde²

2014

Animal

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Early embryo development following fertilization occurs in the oviduct. However, despite being the site of fertilization in cattle, it is possible to by-pass the oviduct by producing embryos in vitro and/or by transferring blastocysts recovered from one female into the uterus of another. While there is substantial evidence for the oviduct having an influence on the quality of the developing embryo, manifested in altered morphology, gene expression and cryotolerance, evidence for a two-way dialogue is weak. In contrast, successful growth and development of the post-hatching blastocyst and pregnancy establishment are a result of the two-way interaction between a competent embryo and a receptive uterine environment. Progesterone (P4) plays a key role in reproductive events associated with establishment and maintenance of pregnancy through its action on the uterine endometrium. Elevated concentrations of circulating P4 in the immediate post-conception period have been associated with an advancement of conceptus elongation, an increase in interferon-tau production and, in some studies, higher pregnancy rates in cattle. This review summarizes current knowledge on the communication between the developing embryo and the maternal reproductive tract.

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“…Plasma P4 concentrations gradually increase with the formation of the CL, reaching a maximum value in the mid-dioestrus phase between Day 10 to 14 of oestrous cycle (Kastelic et al, 1990, Siqueira et al, 2009. Larger variations in expression of immune genes have been observed in endometrial transcriptome profile from cows in mid-dioestrus (Day 12) compared to early metoestrus (Day 3.5) (Mitko et al, 2008). However, in this case, the difference in P4 concentration was not sufficient to stimulate changes in gene expression of these targeted genes.…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, endometrial transcriptome studies (Bauersachs et al, 2005, Mitko et al, 2008, Forde et al, 2011a identified genes differentially expressed during the oestrous cycle. These genes are especially related to cell motility, cytoskeleton, extracellular matrix (ECM), ECM remodelling, and cell growth are differentially expressed.…”

Section: Embryo Mortality and Endometrial Receptivitymentioning

confidence: 99%

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

Satake

Lyons

et al. 2017

Reprod. Fertil. Dev.

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In cattle, early embryonic loss during the first two to three weeks after fertilisation is recognised as the major cause of reproductive failure, with consequent significant impacts on business performance of beef and dairy farms. Inadequate endometrial receptivity is considered an important cause of early embryonic mortality in cattle. A pilot experiment was conducted to compare the outcome of performing different endometrial sampling procedures in tropically adapted (Bos indicus x Bos taurus) heifers, and to select the most suitable method to collect samples for investigation of endometrial gene expression. Endometrial biopsy (EB) using a biopsy device with a diameter similar to an artificial insemination gun (10366L Karl Storz®, Germany) was found to be the most reliable method of collecting endometrial samples in these heifers, with the samples recovered yielding a mean concentration of RNA of 72.9 ±39.3 ng/µl and mean RNA integrity number (RIN) of 7.3 ±1.1.To assess the likely safety of conducting EB in cattle a systematic review of published information assessing the impact of EB on subsequent pregnancy rates was conducted. Using the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) protocol, online searches identified 1164 possible studies. After applying the exclusion criteria, six studies allowed the comparison of pregnancy rates between sampled (n= 1,208) and non-sampled cows (n=2,393). The results showed that EB can be performed before breeding without adversely affecting the likelihood of cows becoming pregnant.A major objective of the research was to determine whether EB and evaluation of endometrial gene expression could be used to identify tropically adapted embryo transfer (ET) recipients more likely to become pregnant. Three potential EB scenarios were investigated, 1) EB on Day 7 of the oestrous cycle prior to the cycle when ET would be performed, 2) EB on Day 4 of the oestrous cycle in which ET would be performed i.e. EB 3 days before ET, and 3) EB on the same day when ET would be performed.The first study assessed the morphological and inflammatory changes induced by endometrial biopsy. Pre-synchronised heifers underwent EB from the horn ipsilateral to the corpus luteum (CL) either at Day 7 of the previous cycle (Group 1 n=10) followed by administration of a luteolytic dose of prostaglandin F2α or at Day 4 (Group 2, n=7) of the present cycle. All heifers were slaughtered on Day 7 of the present cycle. The site of EB was visually evident in 40% (4/10) of Group 1 and 42% (3/7) of Group 2 heifers. Histological assessment revealed no evidence of changes indicative of endometritis in either the biopsied or non-biopsied horns in both groups. The histological iii changes observed included mild mononuclear infiltration of the epithelium and mild vascular changes associated with haemorrhage and congestion.Initial investigation of the endometrial expression of the pro-inflammatory cytokine IL1β and wound healing cytokine TGFβ genes found no significant difference betwe...

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Dynamic changes in messenger RNA profiles of bovine endometrium during the oestrous cycle

Cited by 114 publications

References 71 publications

The endometrium responds differently to cloned versus fertilized embryos

The endometrium responds differently to cloned versus fertilized embryos

Maternal-embryo interaction leading up to the initiation of implantation of pregnancy in cattle

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

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