The SNP Genotypes of Growth Hormone Gene Associated with Reproductive Traits in Tsaiya Ducks

Chang, Mu-Tzu; Cheng, Y. S.; Huang, Mu-Chiou

doi:10.1111/j.1439-0531.2011.01918.x

Cited by 16 publications

(10 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other researchers have observed genotypic effects on production traits [21–24]. In our study, two SNP mutations were detected in exon 2 of the GH gene and 10 genotypes were obtained, consistent with previous studies [20].…”

Section: Resultssupporting

confidence: 91%

Association of Polymorphisms of Exon 2 of the Growth Hormone Gene with Production Performance in Huoyan Goose

Zhang

Zhu

et al. 2014

IJMS

View full text Add to dashboard Cite

Primers based on the cDNA sequence of the goose growth hormone (GH) gene in GenBank were designed to amplify exon 2 of the GH gene in Huoyan goose. A total of 552 individuals were brooded in one batch and raised in Liaoning and Jiangsu Provinces, China. Single nucleotide polymorphisms (SNPs) of exon 2 in the GH gene were detected by the polymerase chain reaction (single strand conformation polymorphism method). Homozygotes were subsequently cloned, sequenced and analyzed. Two SNP mutations were detected, and 10 genotypes (referred to as AA, BB, CC, DD, AB, AC, AD, BC, BD and CD) were obtained. Allele D was predominant, and the frequencies of the 10 genotypes fit the Hardy-Weinberg equilibrium in the male, female and whole populations according to the chi-square test. Based on SNP types, the 10 genotypes were combined into three main genotypes. Multiple comparisons were carried out between different genotypes and production traits when the geese were 10 weeks old. Some indices of production performance were significantly (p < 0.05) associated with the genotype. Particularly, geese with genotype AB or BB were highly productive. Thus, these genotypes may serve as selection markers for production traits in Huoyan geese.

show abstract

Section: Resultssupporting

confidence: 91%

Association of Polymorphisms of Exon 2 of the Growth Hormone Gene with Production Performance in Huoyan Goose

Zhang

Zhu

et al. 2014

IJMS

View full text Add to dashboard Cite

show abstract

“…Early in the 1970s, duck growth hormone (dGH) was purified and identified (Chen et al, 1988;Papkoff and Hayashida, 1972). However, research on the duck growth neuroendocrine regulation mechanism has focused on the detection of single nucleotide polymorphisms (Xu et al, 2007;Chang et al, 2012;Wu et al, 2012), expression patterns (Zhao et al, 2004) and hormone levels (Ge et al, 2000) in single genes, and these studies lacked reproducibility and were not systematic. In China, the results of growth neuroendocrine-regulation research on domestic chickens have been applied as a guide for waterfowl production for many years, although these studies were unscientific and lacked pertinence.…”

Section: Introductionmentioning

confidence: 97%

Profiles of mRNA expression of related genes in the duck hypothalamus–pituitary growth axis during embryonic and early post-hatch development

Liu

Song

et al. 2015

Gene

View full text Add to dashboard Cite

“…Also, Nie et al [30] found a total of 46 SNPs of which, 5 in exons of GH gene of four divergent chicken breeds. In the research made by Chang et al [25], they found 19 SNPs in a region of 2087 base pairs (bp) in the Tsaiya ducks GH gene of which three coding SNPs were recorded. However, Kansaku et al [19] stated that five single nucleotide polymorphisms (SNPs) were found in the promoter region of the duck GH gene.…”

Section: Discussionmentioning

confidence: 99%

“…One pair of gene-specific primer was used in this experiment according to GH gene sequence (Gene Bank accession no. : AB158760) that had been used before by Chang et al [25].…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Growth Hormone Gene Polymorphisms in Mature Healthy Male Muscovy Ducks in Egypt

ELaraby

Awad

Saleh

et al. 2020

Zagazig Veterinary Journal

View full text Add to dashboard Cite

Blood samples were obtained from 28 mature healthy male Muscovy ducks for screening of a partial sequence of Growth hormone gene (GH gene) for single nucleotide polymorphisms using PCR amplification of the extracted DNA. The results of our research revealed one locus of exon 2 of growth hormone gene and its partial flanking intron. Polymorphisms in this locus were investigated by using direct sequencing and single strand conformational polymorphism (SSCP) technique. One unique pattern of SSCP was detected. Nucleotide sequencing was performed to endorse the current result which proved the absence of single nucleotides' polymorphisms (SNPs) or any other type of polymorphisms in the studied locus. The DNA sequencing and PCR-SSCP patterns confirmed that there was no polymorphism in all studied birds. In turn, it is spelled that PCR-SSCP failed to produce electrophoresis patterns capable of discriminating between the GH gene loci in Muscovy duck breed revealing no polymorphism in all studied individuals. Also, we compared our sequences with the published sequence on the gene bank of GH gene in Mallard ducks under the following accession number AB158762.1. Three SNPs were detected between Muscovy and Mallard breeds which are (94 A-G, 271 C-T and 298 G-A) these SNPs led to change of two amino acids proline and valine amino acids in GH protein in mallard ducks into serine and isoleucine in Muscovy ducks. In conclusion, these changes in the nucleotide sequence, amino acid and protein chain may be the main cause of differences of the phenotypic features, productive and reproductive traits between Muscovy and mallard duck breeds.

show abstract

The SNP Genotypes of Growth Hormone Gene Associated with Reproductive Traits in Tsaiya Ducks

Cited by 16 publications

References 30 publications

Association of Polymorphisms of Exon 2 of the Growth Hormone Gene with Production Performance in Huoyan Goose

Association of Polymorphisms of Exon 2 of the Growth Hormone Gene with Production Performance in Huoyan Goose

Profiles of mRNA expression of related genes in the duck hypothalamus–pituitary growth axis during embryonic and early post-hatch development

Growth Hormone Gene Polymorphisms in Mature Healthy Male Muscovy Ducks in Egypt

Contact Info

Product

Resources

About