The skin in magnesium-deficient rats

Davis, Heather L.; Gascho, C.; Kiernan, J. A.

doi:10.1007/bf00561528

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…Assessment of mast cells. The histological procedure for estimating proportions of degranulating mast cells in the skin has been used in several other studies [Kiernan, 1971[Kiernan, , 1972[Kiernan, , 1975Davis, Gascho and Kiernan, 1975]. It should be noted that a mast cell is considered to be degranulating even if only a single extruded granule is seen in a section [Kiernan, 1972].…”

Section: Validity Of Methodsmentioning

confidence: 99%

A Study of Chemically Induced Acute Inflammation in the Skin of the Rat

Kiernan

1977

Exp Physiol

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Oedema due to application of benzene to the skin was reduced following prior sensory denervation and in animals systemically pre-treated with capsaicine (which is known to confer resistance to chemical irritants) or compound 48/80 (which depletes the body of mast cells). Increased degranulation of mast cells in the benzene-treated skin was unaffected by denervation but did not occur after treatment with capsaicine. Antidromic stimulation of a cutaneous nerve caused oedema and degranulation of mast cells, both of which were less severe than the corresponding effects of topically applied benzene. These effects were completely prevented by prior treatment with capsaicine and the oedema was less severe in 48/80-treated rats. Hence, the presence of mast cells was necessary for full development of the effects of antidromic stimulation. These observations indicate that axon reflexes in sensory fibres contribute to, but are not entirely responsible for, the development of oedema in chemically irritated skin. The prophylactic action of capsaicine may be due to the prevention of degranulation of mast cells rather than to a direct effect on cutaneous nerve endings. These conclusions are embodied in an hypothesis purporting to explain the involvement of axon reflexes, mast cells and various humoral mediators in chemically induced acute inflammation.

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Section: Validity Of Methodsmentioning

confidence: 99%

A Study of Chemically Induced Acute Inflammation in the Skin of the Rat

Kiernan

1977

Exp Physiol

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Degranulation of dermal mast cells: Effects of fixation and of antidromic nervous impulses on two histochemically identified cell-types

Lampe

Kiernan

1976

Arch. Derm. Res.

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Two types of mast cell can be identified histochemically in the dermis of the rat's external ear. One type is recognized by the binding of concanavalin A (con A) to the cytoplasmic granules (con A-positive cells) while in the other type (con A-negative cells), the granules do not bind con A. The granules in both types are stained metachromatically by toluidine blue. Antidromic stimulation of the great auricular nerve for 2 min results in an increased proportion of degranulating mast cells in the auricular dermis and both types of cell are affected to an approximately equal extent. In discussion of this observation, it is argued that both the con A-positive and the con A-negative mast cells are probably involved in the mediation of vasodilatation due to axon reflexes in injured skin. The proportions of degranulating mast cells determined in histological preparations varied with the fixatives (Carnoy and glutaraldehyde-formaldehyde) used, but the increased degranulation due to antidromic nervous stimulation could be detected after either fixation.

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Vascular permeability and axonal regeneration in tissues autotransplanted into the brain

Heinicke

1980

Acta Neuropathol

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Pieces of skin, peripheral nerve, muscle, tendon, thyroid gland, and submandibular gland were autotransplanted into the brains of mice. The animals were killed after 5-week periods. Fluorescently labelled albumin was injected i.v. 1 h prior to death. Silver-impregnated sections were examined under the light microscope for the regeneration of axons from the brains into the implanted tissues. Unstained sections were studied by fluorescence microscopy for the presence of the labelled tracer in the extracellular spaces of the grafts. The muscle and submandibular gland received the fewest regenerating axons, skin and tendon received an intermediate amount of reinnervation, and the thyroid gland and vagus nerve were the most richly innervated. The amount of reinnervation could be roughly correlated with the presence of extravascular protein within the tissues. These data support the hypothesis that regeneration of axons may be dependent upon a source of protein in the extracellular fluid surrounding their growing tips.

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The skin in magnesium-deficient rats

Cited by 3 publications

References 10 publications

A Study of Chemically Induced Acute Inflammation in the Skin of the Rat

A Study of Chemically Induced Acute Inflammation in the Skin of the Rat

Degranulation of dermal mast cells: Effects of fixation and of antidromic nervous impulses on two histochemically identified cell-types

Vascular permeability and axonal regeneration in tissues autotransplanted into the brain

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