The Sirtuin 3 Expression Profile Is Associated with Pathological and Clinical Outcomes in Colon Cancer Patients

Liu, Chunyuan; Huang, Zong-hai; Jiang, Hong; Shi, Fujun

doi:10.1155/2014/871263

Cited by 47 publications

(49 citation statements)

References 41 publications

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“…Also, downregulation of SIRT3 in oral cancer cells inhibits cell growth and proliferation as well as enhances radiotherapeutic and chemotherapeutic drug cytotoxicity (Alhazzazi et al, 2011). Similarly, silencing of SIRT3 was shown to inhibit the proliferation, invasion, migration and increased the apoptosis in the cultured colon cancer cell lines (Liu et al, 2014). Thus, based on available data, it appears that SIRT3 can act either as a tumor promoter or as a tumor suppressor (Chen et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Pro-Proliferative Function of Mitochondrial Sirtuin Deacetylase SIRT3 in Human Melanoma

George

Nihal

Singh

et al. 2016

Journal of Investigative Dermatology

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Melanoma, the most aggressive forms of skin cancer, is often fatal if not treated early. Therefore, novel target-based strategies are required to combat this neoplasm. The objective of this study was to determine the role and functional significance of the mitochondrial sirtuin SIRT3 in melanoma. We found that compared to normal primary and immortalized human melanocytes, SIRT3 is significantly overexpressed in multiple human melanoma cells at mRNA and protein levels. Further, employing human tissue microarray, we found that SIRT3 is significantly upregulated in clinical melanoma tissues, compared to melanocytic nevi tissues. Furthermore, a short hairpin RNA (shRNA)-mediated knockdown of SIRT3 in human melanoma cells resulted in 1) decrease in cellular proliferation, colony formation and cellular migration, 2) induction of senescence as shown by increase in SA-β-Gal activity and formation of SAHF as well as increase in mRNA and protein levels of p16INK4a and p21Waf1, 3) G1-phase arrest of the cell cycle, and 4) decreases in mRNA and protein levels of Cyclins (D1, E1) and Cdks (2, 4, 6). Conversely, forced exogenous overexpression of SIRT3 promoted increase in proliferative potential of Hs294T melanoma cells and normal immortalized Mel-ST melanocytes. Finally, we found that SIRT3 knockdown significantly inhibited tumorigenesis in a xenograft model in vivo. To our knowledge, this is the first study supporting the pro-proliferative function of SIRT3 in melanoma.

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Section: Discussionmentioning

confidence: 99%

Pro-Proliferative Function of Mitochondrial Sirtuin Deacetylase SIRT3 in Human Melanoma

George

Nihal

Singh

et al. 2016

Journal of Investigative Dermatology

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“…Colon cancer refers to the malignant lesions of colonic epithelium due to various environmental or genetic carcinogenic factors, ranking third most common among gastrointestinal tumors and also one of the most common malignant tumors (1). The majority of colon cancers is sporadic, and caused by risk factors, including aging, sex, environmental factors, obesity, diabetes, high fat diet, sitting for prolonged durations, insufficient intake of cellulose, smoking and drinking (2).…”

Section: Introductionmentioning

confidence: 99%

Wogonoside inhibits cell growth and induces mitochondrial-mediated autophagy-related apoptosis in human colon cancer cells through the PI3K/AKT/mTOR/p70S6K signaling pathway

et al. 2018

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Abstract. Wogonoside, the main effective constituent of traditional Chinese medicine Scutellaria, belongs to the glucuronide family, with various functions, including detoxification, anti-inflammation and nourishing gallbladder, lowering blood pressure, diuresis and anti-allergic reactions. However, the effects of wogonoside on human colon cancer cells remain unclear. The present study aimed to investigate the anticancer effect of wogonoside on human colon cancer cells in vitro and its anticancer mechanisms. The results demonstrated that wogonoside significantly inhibited cell growth, induced apoptosis and mitochondrial-mediated autophagy of colon cancer cells. Furthermore, the results revealed that wogonoside significantly increased caspase-3 and caspase-9 expression levels, induced apoptosis regulator Bax/Bcl-2 and microtubule-associated protein 1A/1B-light chain 3 protein expression, suppressed the phosphatidylinositol 3 kinase (PI3K)/RAC-α serine/threonine-protein kinase (Akt)/mechanistic target of rapamycin (mTOR)/p70 S6 kinase (p70S6K) signaling pathway and induced p62 protein expression in colon cancer cells. In conclusion, these results demonstrated that wogonoside inhibits cell growth and induces mitochondrial mediated autophagy-related apoptosis in human colon cancer cells through modulation of the PI3K/Akt/mTOR/p70S6K signaling pathway.

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“…At present the SCI mechanism is divided into primary and secondary SCI (3). The former consists of instantaneous mechanical damage that is reversible.…”

Section: Introductionmentioning

confidence: 99%

Tocotrienol alleviates inflammation and oxidative stress in a rat model of spinal cord injury via suppression of transforming growth factor-β

Xun

Mamat

Guo

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. In recent years accumulating evidence has indicated that tocotrienol exhibits an oxidation resistance function, decreased cholesterol function, inhibits cancer function and has unique physiological functions, including anti-inflammatory, anti-apoptotic and anti-oxidative properties. The present study investigated the effect of tocotrienols on spinal cord injury (SCI) by evaluating oxidative stress, inflammation and inducible nitric oxide synthase (iNOS) in rats. A rat model of SCI was induced by operation. SCI rats were treated with 120 mg/kg/day tocotrienol once a day for eight consecutive weeks. Functional recovery following SCI was measured by using the Basso Beattie Bresnahan (BBB) locomotor rating scale. Then the volume of spinal cord contusions was measured following induction of SCI in the rats. In SCI rats, serum malondialdehyde, superoxide dismutase, catalase, glutathione peroxidase, nuclear factor-κB p65 unit, tumor necrosis factor-α, interleukin (IL)-1β and IL-6 levels were analyzed using respective commercial immunoassay kits. Firstly, iNOS, transforming growth factor (TGF)-β, collagen type IV and fibronectin protein expression levels, in addition to iNOS activity and plasma nitric oxide (NO) production in SCI rats was analyzed using western blot analysis, commercial kits and Griess reagent, respectively. Tocotrienol treatment elevated BBB scores and contused volume in the SCI rats. Tocotrienol protected against SCI with reduced oxidative stress and inflammation, and inhibited iNOS protein expression iNOS activity and plasma NO production in rats. In addition, treatment with tocotrienols suppressed TGF-β, collagen type IV and fibronectin protein expression levels in SCI rats. These results suggest that tocotrienols protect SCI, and suppress oxidative stress, inflammation and iNOS in this model of SCI through TGF-β, collagen type IV and fibronectin signaling pathways.

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The Sirtuin 3 Expression Profile Is Associated with Pathological and Clinical Outcomes in Colon Cancer Patients

Cited by 47 publications

References 41 publications

Pro-Proliferative Function of Mitochondrial Sirtuin Deacetylase SIRT3 in Human Melanoma

Pro-Proliferative Function of Mitochondrial Sirtuin Deacetylase SIRT3 in Human Melanoma

Wogonoside inhibits cell growth and induces mitochondrial-mediated autophagy-related apoptosis in human colon cancer cells through the PI3K/AKT/mTOR/p70S6K signaling pathway

Tocotrienol alleviates inflammation and oxidative stress in a rat model of spinal cord injury via suppression of transforming growth factor-β

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