The simultaneous fluorimetric microdetermination of cortisol and corticosterone in plasma

Stewart, C. P.; Albert‐Recht, F.; Osman, L

doi:10.1016/0009-8981(61)90116-4

Cited by 47 publications

(8 citation statements)

References 13 publications

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“…At least 24 hours elapsed between procedures, which commenced at 9.30 a.m. and were carried out in random sequence. Blood samples were collected by repeated venepunctures into heparinized tubes and plasma cortisols were determined using the method of Stewart, Albert-Recht, and Osman (1961) by one of us (D.B.C.) who was unaware of the source of individual blood samples.…”

Section: Methodsmentioning

confidence: 99%

Endocrine function in multiple sclerosis.

Millac¹,

Book²,

Chase³

1969

Journal of Neurology, Neurosurgery & Psychiatry

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Section: Methodsmentioning

confidence: 99%

Endocrine function in multiple sclerosis.

Millac¹,

Book²,

Chase³

1969

Journal of Neurology, Neurosurgery & Psychiatry

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“…In the homogenate of the adrenals and in the heparinized blood plasma, the content of cortisol and corticosterone was determined by the fluorometric method of Stewart et al (1961). This method was used with some slight modifications.…”

mentioning

confidence: 99%

“…According to the results of Stewart et al (1961) the increase in the intensity of fluorescence within the 2nd to the 45th min depends on the combined effects of fluorescence of cortisol, corticosterone and unspecific fluorescence, from the 45th min to the 85th min it depends on the fluorescence of corticosterone and unspecific fluorescence, while from the 85th min to the 120th min it depends only on the unspecific fluorescence. On the basis of these findings, the final calculations were made according to Stewart et al (1961).…”

mentioning

confidence: 99%

Effects of Muscular Work on Cortisol and Corticosterone Content in the Blood and Adrenals of Guinea Pigs

Viru

Äkke

1969

Acta Endocrinologica

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The cortisol and corticosterone content was determined fluorometrically in the blood and in the adrenals of guinea pigs. After three 5-minute\x=req-\ swimming sets the corticoid content increased in the adrenals, but remained unchanged in the blood. After 5-minute-swimming sets repeated until exhaustion, the adrenal corticoid content did not differ from the level of the control group. The blood cortisol content, however, fell significantly. The blood corticosterone content did not change. If before the swimming sets 4 IU of corticotrophin were administered, the cortisol content both in the adrenals and blood at the point of exhaustion was somewhat higher than in the control group. The decrease in blood corticoid concentration during long-lasting muscular work is due to insufficient stimulation of the adrenocortical activity. It has been suggested that the inhibition of the stimulation of the adrenocortical activity is one manifestation of a general defence reaction, against the fatal depletion of the resources of the organism.Determinations of the adrenocortical hormones and their metabolites in urine and blood during muscular exercise have not given constant results. Usually, during long-lasting exercise, a decrease in the excretion (Rivoire et al. 1953;Thorn et al. 1953;Bugard et al. 1961) and in the blood concentration of the corticoids (Stachelin et al. 1955;Cornil et al. 1965) is observed and this is considered to be connected with fatigue. The decreased excretion and corticoids concentration in the plasma is preceded by a period of augmented excretion

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“…At the time of blood sampling, the test subject was in a basal condition (fasting, bed-rest for the previous 12 hours). For the determination of plasma cortisol, a fluorescence method was used, somewhat modified from that described earlier (Steward, Albert-Recht, and Osman, 1961;De Moor, Osinski, Deckx, and Steeno, 1962;Mattingly, 1962).The following groups were examined: 1. 61 healthy children in whom the blood cortisol level was determined at 8 a.m.…”

mentioning

confidence: 99%

Corticosteroid therapy in children with asthma. Hypophyseo-adrenocortical inhibition.

Kerrebijn¹,

Kroon²,

Harders³

1967

Archives of Disease in Childhood

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This investigation was designed to study the function of the hypophyseo-adrenocortical system in children with asthma, treated with long-term corticosteroids in small doses. Methods and SubjectsThe function of the hypophyseo-adrenocortical system was investigated by the method described by Robinson, Mattingly, and Cope (1962), based on the principle that if the system is functionally normal the blood cortisol level, which is decreased in the course of corticosteroid therapy, returns to normal after steroid withdrawal.In a number of asthmatic children on steroid therapy the blood cortisol level was determined. Steroids were then stopped abruptly, and during the subsequent 7 days the blood cortisol level was determined on three occasions. On the 9th and 10th day, the reactivity of the adrenal cortex was determined following ACTH (Cortrophin-Z, Organon) 1-5 u./kg. intramuscularly on two successive days. This dose of ACTH is one that produces maximal stimulation. The blood cortisol level was determined on both days 4 hours after ACTH had been given, and the level was compared with that of the previous day.The blood cortisol level was always determined at 8 a.m. ACTH, when given, was administered at 8 a.m., in which event the blood cortisol level at noon was determined. At the time of blood sampling, the test subject was in a basal condition (fasting, bed-rest for the previous 12 hours). For the determination of plasma cortisol, a fluorescence method was used, somewhat modified from that described earlier (Steward, AlbertRecht, and Osman, 1961;De Moor, Osinski, Deckx, and Steeno, 1962;Mattingly, 1962).The following groups were examined: 1. 61 healthy children in whom the blood cortisol level was determined at 8 a.m.

show abstract

The simultaneous fluorimetric microdetermination of cortisol and corticosterone in plasma

Cited by 47 publications

References 13 publications

Endocrine function in multiple sclerosis.

Endocrine function in multiple sclerosis.

Effects of Muscular Work on Cortisol and Corticosterone Content in the Blood and Adrenals of Guinea Pigs

Corticosteroid therapy in children with asthma. Hypophyseo-adrenocortical inhibition.

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