The simpler, the better: tissue vascularization using the body’s own resources

Laschke, Matthias W.; Menger, Michael D.

doi:10.1016/j.tibtech.2021.07.002

Cited by 19 publications

(17 citation statements)

References 69 publications

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“…Several key growth factors were proven to be angiogenic factors that improve proliferation and differentiation of endothelial cells. Given that the development and maturation of new blood vessels is crucially dependent on the balanced spatiotemporal interaction of these factors, PRP was much more efficient in promoting formation of stable microvascular networks compared with the application of single recombinant growth factors [ 19 ]. It's reported that the platelets adhered to 3D engineered microvessels could enhance endothelial metabolic processes directly associated with glycolysis, which accounts for over 85% of the total cellular ATP production in endothelial cells in normal blood vessels [ 67 ].…”

Section: Resultsmentioning

confidence: 99%

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Functionalizing multi-component bioink with platelet-rich plasma for customized in-situ bilayer bioprinting for wound healing

Zhao

Wang

Zhang

et al. 2022

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Section: Resultsmentioning

confidence: 99%

“…Platelet-rich plasma (PRP) is the plasma containing high concentrations of platelets (PLTs) obtained after centrifugations of whole blood [ 19 ]. High concentrations of PLTs could accelerate the hemostatic process by promoting the thrombosis and coagulation in wounds.…”

Section: Introductionmentioning

confidence: 99%

Functionalizing multi-component bioink with platelet-rich plasma for customized in-situ bilayer bioprinting for wound healing

Zhao

Wang

Zhang

et al. 2022

Materials Today Bio

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“…Blood-derived growth factor-based therapies have gradually gathered more attention for their potential use in the regenerative medicine field [ 14 , 39 ]. Our approach focuses primarily on locally delivering a physiological mixture of blood-derived growth factor proteins to the injury site [ 16 , 18 ], which may offer a simple and convenient way to support and accelerate wound regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…Current strategies for promoting angiogenesis in acute and chronic wounds are frequently based on the exogenous supply of recombinant angiogenesis factors [ 13 ]. However, the concept of employing the body’s own natural resources towards regeneration has recently gained increased interest [ 14 ]. Our preferred approach focuses on utilizing a physiologically-adjusted growth factor therapy which can be administered using a minimally-invasive method [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Hypoxia Preconditioned Serum (HPS)-Hydrogel Can Accelerate Dermal Wound Healing in Mice—An In Vivo Pilot Study

et al. 2022

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The ability to use the body’s resources to promote wound repair is increasingly becoming an interesting area of regenerative medicine research. Here, we tested the effect of topical application of blood-derived hypoxia preconditioned serum (HPS) on wound healing in a murine wound model. Alginate hydrogels loaded with two different HPS concentrations (10 and 40%) were applied topically on full-thickness wounds created on the back of immunocompromised mice. We achieved a significant dose-dependent wound area reduction after 5 days in HPS-treated groups compared with no treatment (NT). On average, both HPS-10% and HPS-40% -treated wounds healed 1.4 days faster than NT. Healed tissue samples were investigated on post-operative day 15 (POD 15) by immunohistology and showed an increase in lymphatic vessels (LYVE-1) up to 45% with HPS-40% application, while at this stage, vascularization (CD31) was comparable in the HPS-treated and NT groups. Furthermore, the expression of proliferation marker Ki67 was greater on POD 15 in the NT-group compared to HPS-treated groups, in accordance with the earlier completion of wound healing observed in the latter. Collagen deposition was similar in all groups, indicating lack of scar tissue hypertrophy as a result of HPS-hydrogel treatment. These findings show that topical HPS application is safe and can accelerate dermal wound healing in mice.

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“…However, the reassembly of these cells into new microvessels can take more than 1 week 10 , which is not fast enough to guarantee the survival of the grafts in the initial post-transplant phase. To overcome this problem, an increasing number of studies suggest adipose tissue-derived microvascular fragments (MVF) as suitable vascularization units 11 , 12 . MVF are a randomized mixture of functional arterioles, capillaries and venules with a length of ~20–200 µm and surrounding single cells, including macrophages and mesenchymal stem cells (MSC) 13 – 15 .…”

Section: Introductionmentioning

confidence: 99%

Co-transplantation of pancreatic islets and microvascular fragments effectively restores normoglycemia in diabetic mice

et al. 2022

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Insufficient revascularization of pancreatic islets is one of the major obstacles impairing the success of islet transplantation. To overcome this problem, we introduce in the present study a straightforward strategy to accelerate the engraftment of isolated islets. For this purpose, we co-transplanted 250 islets and 20,000 adipose tissue-derived microvascular fragments (MVF) from donor mice under the kidney capsule as well as 500 or 1000 islets with 40,000 MVF into the subcutaneous space of diabetic mice. We found that the co-transplantation of islets and MVF markedly accelerates the restoration of normoglycemia in diabetic recipients compared with the transplantation of islets alone. In fact, the transplantation of 250 islets with 20,000 MVF under the kidney capsule reversed diabetes in 88% of mice and the subcutaneous transplantation of 500 or 1000 islets with 40,000 MVF restored normoglycemia in 100% of mice. Moreover, diabetic mice receiving islets and MVF exhibited plasma insulin levels similar to nondiabetic control animals. Additional immunohistochemical analyses of the grafts revealed a significantly higher number of islet cells and microvessels in the co-transplantation groups. These findings demonstrate that the co-transplantation of islets and MVF is a promising strategy to improve the success rates of islet transplantation, which could be easily implemented into future clinical practice.

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The simpler, the better: tissue vascularization using the body’s own resources

Cited by 19 publications

References 69 publications

Functionalizing multi-component bioink with platelet-rich plasma for customized in-situ bilayer bioprinting for wound healing

Functionalizing multi-component bioink with platelet-rich plasma for customized in-situ bilayer bioprinting for wound healing

Hypoxia Preconditioned Serum (HPS)-Hydrogel Can Accelerate Dermal Wound Healing in Mice—An In Vivo Pilot Study

Co-transplantation of pancreatic islets and microvascular fragments effectively restores normoglycemia in diabetic mice

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