Functionalizing multi-component bioink with platelet-rich plasma for customized in-situ bilayer bioprinting for wound healing

Natural blood plasma-based hydrogels as tumor vaccines delivery systems to enhance biomimetic recruitment of antigen presenting cells for tumor immunotherapy

“…The sodium citrate-anticoagulant plasma used in this work contained citrate that chelated with endogenous Ca 2+ in plasma to block blood coagulation. However, along with the addition of exogenous Ca 2+ , the coagulation process would be restarted to trigger the agglutination reaction, promote the production of insoluble fibrin, and finally form a hydrogel [ 19 ]. As shown in Fig.…”

3D printing of cell-delivery scaffolds for tissue regeneration

“…Furthermore, the authors fully demonstrated the healing effects of the multicellular scaffolds for treating both acute and chronic wounds via three animal models. Zhao et al [ 70 ] prepared platelet-rich plasma (PRPs)-containing 3D bioprinted constructs loaded dermal fibroblasts (DFs) and epidermal stem cells (ESCs). With the PRPs incorporation at concentration of 5%, the multicellular constructs were in situ printed on the wound beds and performed the most adequate reepithelization and fastest wound closure during a 21-day healing process.…”

Bioinks adapted for in situ bioprinting scenarios of defect sites: a review

“…Many in situ bioprinting studies have focused on repairing skin defects, and some progress has been made in animal experiments. 53,131 Zhao et al 132 integrated platelet-rich plasma (PRP) at a concentration of 5% into an alginategelatin (AG) composite hydrogel for in situ extrusion bioprinting of full-thickness rat skin defects and found that the addition of PRP improved the cellular behavior of seed cells, regulated the tube formation and macrophage polarization of vascular endothelial cells in a paracrine manner, accelerated highquality wound closure, regulated inammation and initiated angiogenesis compared with AG bioink alone (Fig. 3).…”