The Simple Chromatographic Chamber and Its Application in Circular and Linear TLC

Berezkin, V. G.; Chausov, A. V.

doi:10.1080/10826076.2011.597077

Cited by 1 publication

(1 citation statement)

References 3 publications

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“…This system saved significant time and gave better resolution than classical TLC. The present system of feeding solvent to the plate is much simpler and faster than classical ascending TLC, making it perfect for high-performance TLC [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Separation of Amino Acids, Dyes, and Pigments Using Novel Pressurized Circular TLC Assembly for Secure Medical Imaging Applications

Dar

Ahmad

Samin

et al. 2023

International Journal of Analytical Chemistry

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A novel pressurized flow system for circular thin-layer chromatography (PC-TLC) has been successfully established and employed for the separation of amino acids, dyes, and pigments for safe medical imaging applications. In this system, the mobile phase is applied to a regular TLC plate through the tube and needle of an intravenous infusion set. The needle was fused in a hole underneath the center of the plate, while the second side end of the tube was connected to a microburette containing the solvent. This new assembly proved itself better in terms of separation time (within 5 minutes) and controlled flow of the solvent and horizontal movement of analyte components over chromatograms with better separation and Rf values (glutamine: 0.26, valine: 0.44, phenylalanine: 0.60, chlorophyll a: 0.52, chlorophyll b: 0.43, xanthophyll: 0.18, carotenoid: 0.97, and pheophytin: 0.60) when a number of samples of amino acids, dyes, and pigments were separated by the developed apparatus and the conventional TLC procedure. The developed method was found distinctly rapid, precise, and eco-friendly (less solvent consuming) as compared to traditional ascending TLC.

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Section: Introductionmentioning

confidence: 99%