The silkworm (Bombyx mori) neuropeptide orcokinin is involved in the regulation of pigmentation

Wang, Pingyang; Zhao, Qiaoling; Qiu, Zhiyong; Bi, Simin; Wang, Wenbo; Wu, Meina; Chen, Anli; Xia, Dajing; He, Xiaobai; Tang, Shengnan; Li, Muwang; Zhang, Guozheng; Shen, Xingjia

doi:10.1016/j.ibmb.2019.103229

Cited by 13 publications

(6 citation statements)

References 40 publications

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“…The plasmid pBac ( IE1 ‐ DsRed2‐U6‐sgRNA) (U6‐sgRNA) was developed to induce sgRNA to influence the silkworm regulator, and the DsRed fluorescence marker gene was under the control of the IE1 regulator. The sequences containing sgRNA were constructed to validate the apparent off‐target attachment employed by CRISPR Direct (http://crispr.dbcls.jp/) by conducting thorough queries across silkworm genomic sequences (Wang et al ., 2019). Table S1 presents both the sgRNA and oligonucleotide primer sequences used for plasmid construction.…”

Section: Methodsmentioning

confidence: 99%

CRISPR/Cas9‐based knockout reveals that the clock gene timeless is indispensable for regulating circadian behavioral rhythms in Bombyx mori

et al. 2020

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Circadian rhythms, which are ubiquitous and adaptive, occur across all species, from microbes to humans, in which they organize and modify behavior and physiology. timeless (tim) is a canonical clock gene. The core composition of the Drosophila melanogaster endogenous circadian clock has been extensively investigated; however, in lepidopteran insects, including Bombyx mori, the mechanism is complicated and little is known regarding the participation of tim in the negative feedback loop responsible for behavioral activities. To arrive at a comprehensive understanding of the role of tim in the B. mori endogenous circadian clock, we exploited the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 gene editing system. We attempted to elucidate the functions of tim in the circadian clock of B. mori using Bmtim mutants. The knockouts affected two circadian behavioral activities: adult emergence and embryo hatching rhythms. Quantitative real-time polymerase chain reaction results confirmed that tim-knockouts induced relative reductions in the expression levels, and thereby the oscillation amplitudes, of Bmper and Bmclk messenger RNAs during both the photophase and scotophase. Additionally, the daily rhythmic expression of Bmdbt was upregulated in the photophase and downregulated in the scotophase in a tim-knockout. Our study reveals that tim is integral to the B. mori circadian clock and may be involved in regulating eclosion and hatching rhythms.

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Section: Methodsmentioning

confidence: 99%

CRISPR/Cas9‐based knockout reveals that the clock gene timeless is indispensable for regulating circadian behavioral rhythms in Bombyx mori

et al. 2020

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“…Insect OKs were proved to have a variety of effects, e.g. regulation of pigmentation 31 , ecdysis 32 , vitellogenin transcription 33 and circadian rhythmicity 34,35 . Knockdown of orcokinin gene of D. melanogaster caused a disinhibition of male courtship behavior, however, it is unclear whether OKA or OKB is involved in the control of the courtship 36 .…”

Section: Discussionmentioning

confidence: 99%

Identification and expression analysis of neuropeptides and their G protein-coupled receptors in the predatory stink bug, Arma custos (Hemiptera: Pentatomidae)

Huang

Xiang

et al. 2023

Preprint

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The predatory stink bug Arma custos has been selected as an effective biological agent and has been successfully massly bred and released into fields for the control of a diverse insect pests. As a zoophytophagous generalist, A. custos relies on a complex neuropeptide signaling system to prey on distinct food and adapt into different environment. However, information about neuropeptide signaling genes in predatory bugs has not been reported to date. In the present study, a total of 57 neuropeptide precursor transcripts and 41 potential neuropeptide GPCR transcripts were found mainly using our sequenced transcriptome data. Furthermore, a set of neuropeptides and their GPCR receptors that were enriched in heads, guts, salivary glands, antennae, and legs of A. custos were identified, which might play critical roles in central nervous regulation, feeding, digestion, odor perception and motor modulation, etc. Our study provides basic information for in-depth understanding of biological and ecological characteristics of the predatory bug and would aid in the development of better pest management strategies based on the effective utilization and protection of beneficial nature enemies.

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“…A pair of sgRNA (1000 ng/μL each) and cas9 protein (20 μmol/L) were mixed by the ratio of 1:1 [ 17 ]. The mixture was injected into the eggs of Qiufeng (diapause-free eggs) within 2 h after oviposition through TransferMan 4r microinjection system (Eppendorf, Germany).…”

Section: Methodsmentioning

confidence: 99%

phytanoyl-CoA dioxygenase domain-containing protein 1 plays an important role in egg shell formation of silkworm (Bombyx mori)

Chen

Liao

et al. 2021

PLoS ONE

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Yun7Ge is a giant egg mutant found in the silkworm variety Yun7. In comparison with the giant mutant Ge, the eggs of Yun7Ge are larger. The number of laid eggs and hatching rate of Yun7Ge are reduced, which is not conducive to reproduction. In this work, the target gene controlling giant egg trait is located on the Z chromosome and was determined through genetic analysis. Transcriptome results showed that phytanoyl-CoA dioxygenase domain-containing protein 1 (PHYHD1) on the Z chromosome was silenced, and the 25 chorion genes on chromosome 2 were remarkably downregulated. Sequence analysis showed that the 73.5 kb sequence including the PHYHD1 was replaced by a ~3.0 kb sequence. After knocking out the PHYHD1 by using CRISPR/Cas9, the chorion genes were significantly downregulated. Hence, the silencing of PHYHD1 leads to the downregulation of many chorion protein genes, thus directly causing giant eggs.

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The silkworm (Bombyx mori) neuropeptide orcokinin is involved in the regulation of pigmentation

Cited by 13 publications

References 40 publications

CRISPR/Cas9‐based knockout reveals that the clock gene timeless is indispensable for regulating circadian behavioral rhythms in Bombyx mori

CRISPR/Cas9‐based knockout reveals that the clock gene timeless is indispensable for regulating circadian behavioral rhythms in Bombyx mori

Identification and expression analysis of neuropeptides and their G protein-coupled receptors in the predatory stink bug, Arma custos (Hemiptera: Pentatomidae)

phytanoyl-CoA dioxygenase domain-containing protein 1 plays an important role in egg shell formation of silkworm (Bombyx mori)

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