The Significance of Gelatin in Calcium Phosphate Hybrid Bone Cement for Attachment and Differentiation of MG63 Cells

Abstract

Natural polymeric gelatin can improve the handling properties of self‐setting CPCs, which are used in the repair of bone defects. This study is aimed at examining the effect of gelatin on the biological functions of MG63 osteoblast‐like cells. Gelatin‐containing CPCs show greater cellular viability than a CPC control without gelatin and significantly more FN is absorbed. Cell proliferation, with the amount of doped gelatin, and the expression of activated FAK and total integrin, with increasing gelatin content, are increased. The highest levels of total integrin, pFAK, FN, osteocalcin, bone sialoprotein, and calcium deposits are expressed by MG63 cells on the CPC containing 10% gelatin.

Bio-inspired calcium silicate–gelatin bone grafts for load-bearing applications

“…Biomolecules with OH, COOH, and NH 2 functional groups, such as gelatin, alter the functional presentation of the major integrin-binding domain of adsorbed fibronectin and modulate the integrin binding, localization, and specificity. 25,50 The recognition and binding of the integrin receptors to the functional groups of gelatin on the substrate initiate focal adhesions and subsequently activate FAK by autophosphorylation, 25 the degree of which may consequently influence the spreading, proliferation, and differentiation of the cells.…”

“…23 Naturally polymeric gelatin has been used as a scaffold material in tissue engineering and as a drug carrier for controlled delivery because of its biocompatibility, biodegradability, and non-toxicity. [24][25][26] More importantly, the presence of gelatin can improve the mechanical properties and anti-washout ability of the ceramic cement 10,11,27 due to a better distribution of the mechanical load and lower porosity. Here we prepared novel bone analogs that could match both the mechanical and osteogenic properties of bone by a simple pressing-hydrothermal method.…”

In Vitro Physicochemical Properties, Osteogenic Activity, and Immunocompatibility of Calcium Silicate–Gelatin Bone Grafts for Load-Bearing Applications

“…This may be due to the release of soluble fractions (mainly gelatin). Because gelatin is biocompatible and has been classified as a bioresorbable material, 19 its presence or dissolution is not expected to cause biological problems. Immersed CSG15 reached its maximal weight loss of 18% on day 180.…”

“…Thus, the beneficial effects of gelatin on cellular functions may be due to the role of adhesion. 19 To elucidate the effects of gelatin on osteogenic activity, the biological functions of pulp cells cultured on various gelatincontaining specimens were evaluated. The number of cells initially attached was different between the bone grafts with and without gelatin.…”

Robocasting of biomimetic hydroxyapatite scaffolds using self-setting inks

“…15 In addition, the combination of gelatine with calcium phosphate cements has been shown to yield signicant benets both in the processability and enhancement of the mechanical properties [15][16][17][18][19] and in the biological response. [20][21][22][23] Our hypothesis was that the gelling capacity of gelatine could also provide the viscoelasticity and the support needed for the build-up of the 3D-structure, while mimicking also the composite nature of bone, as it comes from the denaturation of collagen. 24 Therefore, in this study, a reactive ink based on gelatine combined with a-TCP is presented and its application for robocasting technology is demonstrated.…”