The signal flow and motor response controling chemotaxis of sea urchin sperm

Kaupp, U. Benjamin; Solzin, Johannes; Hildebrand, Eilo; Brown, J. E.; Helbig, Annika; Hagen, Volker; Beyermann, Michael; Pampaloni, Francesco; Weyand, Ingo

doi:10.1038/ncb915

Cited by 191 publications

(342 citation statements)

References 29 publications

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“…Wave asymmetry seems to be important for the regulation of swimming direction of the sperm of various organisms. Sperm of some sea urchins and those of the tunicate, Ciona, are known to show chemotactic responses to certain egg-related substances; that is, they sense the attracting factors released from the egg and change the asymmetry of flagellar waveforms (Kaupp et al, 2003;Wood et al, 2005;Shiba et al, 2008). Chemotaxis, however, is not the sole response of swimming sperm that depends on Ca 2+ -regulation of flagellar movement.…”

Section: Camentioning

confidence: 99%

Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa

Kambara

Shiba

Yoshida

et al. 2011

Cell Struct. Funct.

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ABSTRACT. Flagellar movement of the sea urchin sperm is regulated by intracellular Ca 2+ . Flagellasialin, a polysialic acid-containing glycoprotein, as well as other membrane proteins seems responsible for the Ca 2+ control. To elucidate the mechanism of Ca 2+ dynamics underlying flagellar movement, we analysed the sperm's mechanosensory behavioural responses by using microtechniques. In sea water containing 10 mM Ca 2+ , the sperm swim in circular paths. When a mechanical stimulus was applied to the sperm head with a glass microstylus, the sperm showed a series of flagellar responses, consisting of a stoppage of beating (quiescence) and a recovery of swimming in a straight path, followed by swimming in a circular path again; as the result the sperm avoided the obstacle. Ca 2+ -imaging with Fluo-4 showed that the intracellular Ca 2+ was high in the quiescence and gradually decreased after that. The effects of blockers and antibodies against candidate components revealed that the Ca 2+ influx was induced by Ca 2+ channels and the Ca 2+ efflux was induced by a flagellasialin-related Ca 2+ -efflux system, plasma membrane Ca 2+ -ATPases and the K + -dependent Na + /Ca 2+ exchanger. The results show that the Ca 2+ -dependent mechanosensory behaviour of the sea urchin sperm is regulated by organized functioning of the membrane environment including the plasma membrane proteins and flagellasialin.

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Section: Camentioning

confidence: 99%

Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa

Kambara

Shiba

Yoshida

et al. 2011

Cell Struct. Funct.

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“…The favorable properties of caged compounds have significantly contributed to study chemotaxis and flagellar modulation in sea urchin sperm (Tatsu et al 2002, Kaupp et al 2003, Bohmer et al 2005, Wood et al 2005, Guerrero et al 2010. In particular, uncaging by a u.v.…”

Section: Introductionmentioning

confidence: 99%

A caged progesterone analog alters intracellular Ca2+ and flagellar bending in human sperm

Servin‐Vences¹,

Tatsu²,

Ando³

et al. 2012

Reproduction

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Progesterone is a physiological agonist for mammalian sperm, modulating its flagellar movement and facilitating the acrosome reaction. To study the initial action of progesterone, we developed a caged analog with a photosensitive group: nitrophenylethanediol, at position 20. Using this compound combined with stroboscopic illumination, we performed Ca 2C imaging of human spermatozoa and analyzed ] i immediately in the flagellum (mid-piece and principal piece), thereafter in the head with a short time lag. This observation is different from the progesterone-induced Ca 2C mobilization in mouse spermatozoa, where the Ca 2C rise initiates at the base of the sperm head. Our finding is mostly consistent with the recent discovery that progesterone activates CatSper channels in human spermatozoa, but not in mouse spermatozoa.

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“…Resact binds directly to a membrane-bound guanylate cyclase receptor in sperm and activates it (Singh et al, 1988;Kaupp et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Conserved sequences of sperm-activating peptide and its receptor throughout evolution, despite speciation in the sea star Asterias amurensis and closely related species

Nakachi

Hoshi

Matsumoto

et al. 2008

Zygote

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Conserved sequences of sperm-activating peptide and its receptor throughout evolution, despite speciation in the sea star Asterias amurensis and closely related species" (2008 SummaryThe asteroidal sperm-activating peptides (asterosaps) from the egg jelly bind to their sperm receptor, a membrane-bound guanylate cyclase, on the tail to activate sperm in sea stars. Asterosaps are produced as single peptides and then cleaved into shorter peptides. Sperm activation is followed by the acrosome reaction, which is subfamily specific. In order to investigate the molecular details of the asterosap-receptor interaction, corresponding cDNAs have been cloned, sequenced and analysed from the Asteriinae subfamily including Asterias amurensis, A. rubens, A. jorbesi and Aphelasterias japonica, as well as Distolasterias nipon from the Coscinasteriinae subfamily. Averages of 29% and 86% identity were found from the deduced amino acid sequences in asterosap and its receptor extracellular domains, respectively, across all species examined. The phylogenic tree topology for asterosap and its receptor was similar to that of the mitochondrial cytochrome c oxidase subunit I. In spite of a certain homology, the amino acid sequences exhibited speciation. Conservation was found in the asterosap residues involved in disulphide bonding and proteinase-cleaving sites. Conversely, similarities were detected between potential asterosap-binding sites and the structure of the atrial natriuretic peptide receptor. Although the sperm-activating peptide and its receptor share certain common sequences, they may serve as barriers that ensure speciation in the sea star A. amurensis and closely related species.

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The signal flow and motor response controling chemotaxis of sea urchin sperm

Cited by 191 publications

References 29 publications

Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa

Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa

A caged progesterone analog alters intracellular Ca2+ and flagellar bending in human sperm

Conserved sequences of sperm-activating peptide and its receptor throughout evolution, despite speciation in the sea star Asterias amurensis and closely related species

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