Conserved sequences of sperm-activating peptide and its receptor throughout evolution, despite speciation in the sea star <i>Asterias amurensis</i> and closely related species

Nakachi, Mia; Hoshi, Motonori; Matsumoto, Midori; Moriyama, Hideaki

doi:10.1017/s0967199408004759

Cited by 10 publications

(11 citation statements)

References 29 publications

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“…Electrophoretic bands representing dissociated α- and β-type chain monomers were excised from each AUT gel, digested with trypsin, and identified by means of tandem mass spectrometry (MS/MS; [57,58]). The peak lists of the MS/MS data were generated by Distiller (Matrix Science, London, UK) using the charge state recognition and de-isotoping with default parameters for quadrupole time-of-flight data.…”

Section: Methodsmentioning

confidence: 99%

Molecular basis of a novel adaptation to hypoxic-hypercapnia in a strictly fossorial mole

et al. 2010

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BackgroundElevated blood O2 affinity enhances survival at low O2 pressures, and is perhaps the best known and most broadly accepted evolutionary adjustment of terrestrial vertebrates to environmental hypoxia. This phenotype arises by increasing the intrinsic O2 affinity of the hemoglobin (Hb) molecule, by decreasing the intracellular concentration of allosteric effectors (e.g., 2,3-diphosphoglycerate; DPG), or by suppressing the sensitivity of Hb to these physiological cofactors.ResultsHere we report that strictly fossorial eastern moles (Scalopus aquaticus) have evolved a low O2 affinity, DPG-insensitive Hb - contrary to expectations for a mammalian species that is adapted to the chronic hypoxia and hypercapnia of subterranean burrow systems. Molecular modelling indicates that this functional shift is principally attributable to a single charge altering amino acid substitution in the β-type δ-globin chain (δ136Gly→Glu) of this species that perturbs electrostatic interactions between the dimer subunits via formation of an intra-chain salt-bridge with δ82Lys. However, this replacement also abolishes key binding sites for the red blood cell effectors Cl-, lactate and DPG (the latter of which is virtually absent from the red cells of this species) at δ82Lys, thereby markedly reducing competition for carbamate formation (CO2 binding) at the δ-chain N-termini.ConclusionsWe propose this Hb phenotype illustrates a novel mechanism for adaptively elevating the CO2 carrying capacity of eastern mole blood during burst tunnelling activities associated with subterranean habitation.

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Section: Methodsmentioning

confidence: 99%

Molecular basis of a novel adaptation to hypoxic-hypercapnia in a strictly fossorial mole

et al. 2010

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“…The resultant peptides were then identified by means of tandem mass spectrometry (MS/MS) (cf. Nakachi et al, 2008;Campbell et al, 2010;. The peak lists of the MS/MS data were generated by Distiller (Matrix Science, London, UK) using the charge state recognition and de-isotoping with default parameters for quadrupole time-of-flight data.…”

Section: Characterization Of Isohb Diversitymentioning

confidence: 99%

Developmental regulation of hemoglobin synthesis in the green anole lizard Anolis carolinensis

Storz

Hoffmann

Opazo

et al. 2011

Journal of Experimental Biology

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SUMMARYTetrapod vertebrates possess multiple -and -like globin genes that are ontogenetically regulated, such that functionally distinct hemoglobin (Hb) isoforms are synthesized during different stages of development. The -and -like globin genes of amphibians, birds and mammals are differentially expressed during embryonic development and postnatal life, but little is known about the developmental regulation of globin gene expression in non-avian reptiles. Here we report an investigation into the developmental regulation of Hb synthesis in the green anole lizard Anolis carolinensis. We tested two hypotheses derived from comparative genomic studies of the globin gene clusters in tetrapod vertebrates. First, we tested whether the product of the Anolis  D -globin gene is incorporated into embryonic Hb, thereby performing the role that would normally be performed by the embyronic  E -globin gene (which has been deleted from the green anole genome). Second, we tested whether two 'lizard-specific' -globin paralogs have independently evolved a division of labor between an early-expressed embryonic gene and a later-expressed adult gene. Results of a proteomic analysis revealed that -and -like globin genes of the anole are differentially expressed during embryonic development. However, the same repertoire of -and -chain Hb isoforms was expressed during all stages of development and postnatal life, and the ontogenetic shifts in isoform composition were relatively subtle. In contrast to the pattern that has been documented in other tetrapod vertebrates, it appears that the developmental regulation of Hb synthesis in the green anole lizard does not involve discrete, stage-specific switches in gene activation and gene silencing.

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“…De novo assembly of transcriptome data can produce complete coding sequences for gamete recognition proteins and other loci (Tautz et al 2010), and be used to characterize population‐ and species‐level adaptive molecular evolution even in nonmodel organisms for which there is no reference genome sequence or other genomic resources (Barreto et al 2011; Feldmeyer et al 2011). The following example is based on preliminary assemblies of testis and ovary transcriptome data from our ongoing study of population differentiation in the northeastern Pacific bat star Patiria miniata (Keever et al 2009; McGovern et al 2010) A key feature of this example is the use of commercial sequencing services and assembly software (as in Feldmeyer et al 2011) to discover and analyze complete or near‐complete coding sequences for four P. miniata loci previously studied mainly among Japanese species (including Patiria pectinifera ; Hirohashi et al 2008; Nakachi et al 2008; Naruse et al 2011).…”

Section: A High‐throughput Solution: More Genes and Taxa Using Transcmentioning

confidence: 99%

“…Scale bar shows 0.1 substitutions per nucleotide site. Previously published data for Asterias , Aphelasterias , Distolasterias , and Acanthaster species plus P. pectinifera from Nakachi et al (2008) and Naruse et al (2011). (A) Out of the small proportion of total testis sequence reads used for this preliminary assembly (10 6 ), one‐third (320,236) assembled into 819 contigs including one contig encoding the sperm receptor for asterosap, which was highly expressed (4351 sequence reads mapped to this contig; average 93.5× coverage per base).…”

Section: A High‐throughput Solution: More Genes and Taxa Using Transcmentioning

confidence: 99%

Next-Generation Studies of Mating System Evolution

Hart

2012

Evolution

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The specificity of mate selection can vary from wantonly indiscriminate to extraordinarily choosy, and depends in large part on molecules expressed on the surfaces of sperm and eggs. Understanding the evolution of this specificity of gamete recognition leads to important insights into the evolution of reproductive isolation and speciation. One productive area of research has focused on genes that encode gamete recognition proteins in broadcast-spawning marine invertebrates. These gene products are relatively accessible to biochemical and cellular analyses of expression and function, and they mediate almost all of the elements of mate selection and specificity between males and females of such species. However, genetic analyses of their evolution are currently limited to a few combinations of molecules and taxa, and may miss the broader view of adaptive responses to selection on mating specificity across many genes and many types of mating systems. A transcriptomic study shows how next-generation sequencing methods and analyses could relatively easily broaden such studies to more clades, deepen those studies to include more of the interacting molecular parts that mediate gamete recognition, and eventually lead to a more complete understanding of the molecular basis for mating system variation and its evolutionary response to selection. K E Y W O R D S :ARIS, asterosap, bindin, fertilization, guanylate cyclase, lysin.

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Conserved sequences of sperm-activating peptide and its receptor throughout evolution, despite speciation in the sea star Asterias amurensis and closely related species

Cited by 10 publications

References 29 publications

Molecular basis of a novel adaptation to hypoxic-hypercapnia in a strictly fossorial mole

Molecular basis of a novel adaptation to hypoxic-hypercapnia in a strictly fossorial mole

Developmental regulation of hemoglobin synthesis in the green anole lizard Anolis carolinensis

Next-Generation Studies of Mating System Evolution

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