Next-Generation Studies of Mating System Evolution

Hart, Michael W.

doi:10.1111/j.1558-5646.2012.01605.x

Cited by 7 publications

(13 citation statements)

References 32 publications

(51 reference statements)

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“…The first step in this process—initial identification of candidate coding sequence regions under selection—could be difficult to complete using PCR‐based methods that target a small proportion of the genomic structure and more readily studied across taxa and genes using transcriptomic and proteomic methods that take advantage of new high‐throughput sequencing methods and bioinformatics (Findlay & Swanson ; Carstens et al . ; Hart ).…”

Section: Discussionmentioning

confidence: 99%

“…Larger samples of alleles for plausible targets of selection (like the EGF and EBRf domains) are needed to test these conclusions about positive selection on a few EBR1 coding sequence domains and the apparent absence of EBR1 population divergence and to understand the causes of the difference between this pattern and the strong divergence of sperm bindin between the same sea star populations. The first step in this process -initial identification of candidate coding sequence regions under selection-could be difficult to complete using PCR-based methods that target a small proportion of the genomic structure and more readily studied across taxa and genes using transcriptomic and proteomic methods that take advantage of new high-throughput sequencing methods and bioinformatics (Findlay & Swanson 2009;Carstens et al 2012;Hart 2012).…”

Section: Discussionmentioning

confidence: 99%

“…For each library, I assembled 10–20 million paired‐end reads into contigs using the de novo transcriptome assembler in seqman ngen 3.1 (e.g., Feldmeyer et al . ; Hart ) with default trim and quality settings including a mer size of 20, match score of 10, mismatch score of 20 and gap penalty of 30. I then searched GenBank for blastx similarities between the assembled contigs and echinoderm accessions.…”

Section: Methodsmentioning

confidence: 99%

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Structure and evolution of the sea star egg receptor for sperm bindin

Hart

2013

Molecular Ecology

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Selection on coevolving sperm- and egg-recognition molecules is a potent engine of population divergence leading to reproductive isolation and speciation. The study of receptor-ligand pairs can reveal co-evolution of male- and female-expressed genes or differences between their evolution in response to selective factors such as sperm competition and sexual conflict. Phylogeographical studies of these patterns have been limited by targeted gene methods that favour short protein-coding sequences amplifiable by PCR. Here, I use high-throughput transcriptomic methods to characterize the structure and divergence of full-length coding sequences for the gene encoding the protein component of a large complex egg surface glycopeptide receptor for the sperm acrosomal protein bindin from the sea star Patiria miniata. I used a simple but effective method for resolving nucleotide polymorphisms into haplotypes for phylogeny-based analyses of selection. The protein domain organization of sea star egg bindin receptor (EBR1) was similar to sea urchins and included a pair of protein-recognition domains plus a series of tandem repeat domains of two types. Two populations separated by a well-characterized phylogeographical break included lineages of EBR1 alleles under positive selection at several codons (similar to selection on sperm bindin in the same populations). However, these populations shared the same alleles that were under selection for amino acid differences at multiple codons (unlike the pattern of selection for population divergence in sperm bindin). The significance of positively selected EBR1 domains and alleles could be tested in functional analyses of fertilization rates associated with EBR1 (and bindin) polymorphisms.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Structure and evolution of the sea star egg receptor for sperm bindin

Hart

2013

Molecular Ecology

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“…A number of caveats remain when calculating dispersal distances from genetic data (Palumbi 2003, Hellberg 2009). These include inferring a lack of dispersal as the cause of differentiation (Hart andMarko 2010, Marko andHart 2011), the influence of pelagic larval duration (PLD) on dispersal ability (Weersing and Toonen 2009, Selkoe and Toonen 2011, Riginos et al 2011, Faurby and Barber 2012 and realized dispersal on population genetic structure and gene flow.…”

Section: Spatial Planning and Restorationmentioning

confidence: 99%

The application of genetics to marine management and conservation: examples from the Indo-Pacific

Heyden¹,

Beger²,

Toonen³

et al. 2014

BMS

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ABSTRACT.-Molecular tools and analyses have played pivotal roles in uncovering the processes and patterns of biodiversity in the Indian and Pacific oceans. However, integrating genetic results into management and conservation objectives has been challenging, with few examples that show practical applicability. This review aims to address some of the perceived barriers to an enhanced approach that integrates molecular data into management and conservation goals, by reviewing papers relevant to both conservation and fisheries management in the Indo-Pacific region, particularly with respect to phylogeography, connectivity, and species identification, as well as stock delineation, restoration of depleted wild stocks, mislabeled marine resources and "molecular forensics." We also highlight case studies from each of these areas that illustrate how molecular analyses are relevant to conservation and management in the IndoPacific, spanning a variety of vertebrate and invertebrate species. We discuss the application of genetic data to the design and evaluation of the effectiveness of marine protected area networks, stock delineation, and restoration and the usage of exclusion tests and parentage analyses for fisheries management. We conclude that there is a distinct need for increasing public awareness and ownership of genetically unique lineages and, ultimately, the increased inclusion of genetic research into management policy and conservation. Finally, we make a case for the importance of clear and effective communication for promoting public awareness, public ownership, and for achieving conservation goals within the region.

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“…Finally, because of their joint role in sperm–egg interactions and their ecological importance, egg coats may act both as species barriers (e.g. Wong & Wessel ; Turner & Hoekstra ; Palumbi ; Vacquier & Swanson ; Hart ) and be under natural selection (Podolsky ; Räsänen et al . ).…”

Section: Why Egg Coats Mattermentioning

confidence: 99%

Evolution of egg coats: linking molecular biology and ecology

2015

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One central goal of evolutionary biology is to explain how biological diversity emerges and is maintained in nature. Given the complexity of the phenotype and the multifaceted nature of inheritance, modern evolutionary ecological studies rely heavily on the use of molecular tools. Here, we show how molecular tools help to gain insight into the role of egg coats (i.e. the extracellular structures surrounding eggs and embryos) in evolutionary diversification. Egg coats are maternally derived structures that have many biological functions from mediating fertilization to protecting the embryo from environmental hazards. They show great molecular, structural and functional diversity across species, but intraspecific variability and the role of ecology in egg coat evolution have largely been overlooked. Given that much of the variation that influences egg coat function is ultimately determined by their molecular phenotype, cutting-edge molecular tools (e.g. proteomics, glycomics and transcriptomics), combined with functional assays, are needed for rigorous inferences on their evolutionary ecology. Here, we identify key research areas and highlight emerging molecular techniques that can increase our understanding of the role of egg coats in the evolution of biological diversity, from adaptation to speciation.

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Next-Generation Studies of Mating System Evolution

Cited by 7 publications

References 32 publications

Structure and evolution of the sea star egg receptor for sperm bindin

Structure and evolution of the sea star egg receptor for sperm bindin

The application of genetics to marine management and conservation: examples from the Indo-Pacific

Evolution of egg coats: linking molecular biology and ecology

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