“…Next generation sequencing, which produces millions of sequencing reads, can achieve the sequence depth required to elucidate even lowly expressed genes of complex protein families. To exploit these advances, a number of de novo sialotranscriptomes (sets of RNA molecules in tick salivary glands) have recently been generated using NGS technologies for a number of tick species; A. maculatum (Karim et al, 2011), Ixodes ricinus (Schwarz et al, 2013), Dermacentor andersoni (Mudenda et al, 2014), A. triste, A. parvum and A. cajennense (Garcia et al, 2014), Haemaphysalis flava (Xu et al, 2015) and R. pulchellus (Tan et al, 2015a). These transcriptomes highlighted the true expansion within salivary protein families in the different tick species and identified new candidate genes involved in feeding.…”