The sheep erythropoietin gene: molecular cloning and effect of hemorrhage on plasma erythropoietin and renal/liver messenger RNA in adult sheep

Fu, Ping; Evans, Bronwyn A; Lim, Gaik Bee; Wintour, E. M.

doi:10.1016/0303-7207(93)90113-x

Cited by 22 publications

(4 citation statements)

References 27 publications

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“…Erythropoietin (Epo) is a glycoprotein hormone produced by the kidney and liver in the adult human which regulates red cell production (Fisher, 1993). Since the human, monkey, mouse and sheep Epo gene have all been cloned ( Jacobs et al, 1985;Lacombe et al, 1988b;Lin et al, 1985;McDonald et al, 1986;Shoemaker & Mitsock, 1986;Fu et al, 1993), cDNA probes for these species have been developed to localize Epo mRNA. Most previous studies involving the localization of Epo mRNA in the kidneys of mice and rats using in situ hybridization and/or transgenic technology have suggested that peritubular interstitial cells as the source of Epo (Bachmann et al, 1993;Koury et al, 1988Koury et al, , 1989Lacombe et al, 1988a;Schuster et al, 1992;Semenza et al, 1991a;Maxwell et al, 1993); however, two studies have reported that tubular epithelial cells are the source of Epo (Loya et al, 1994;Maxwell et al, 1990).…”

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confidence: 99%

Erythropoietin production by interstitial cells of hypoxic monkey kidneys

et al. 1996

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Previous studies which demonstrated that interstitial cells of the peritubular capillary bed of the kidneys are the site of erythropoietin (Epo) production have been performed in non-primate species. In this study, kidneys from adult rhesus monkeys exposed to 18 h hypoxia (0.42 atm) with high serum (5685 mU/ml) and kidney (814 mU/g. includes serum EPO in the kidney) levels of Epo were compared with a kidney from a nonhypoxic normal rhesus monkey. Localization of Epo mRNA by in situ hybridization was carried out with either anti-sense or sense RNA probes generated from a 645 base pair KpnI-BgIII fragment of a monkey Epo cDNA. Epo mRNA was demonstrated only in interstitial cells in the peritubular capillary bed of the hypoxic and normal monkey kidneys utilizing the antisense probe. The finding that the same type of cell that produces EPO in mice, rats and sheep also produces EPO in a higher primate species strongly supports the contention that renal interstitial cells also produce EPO in the human.

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Erythropoietin production by interstitial cells of hypoxic monkey kidneys

et al. 1996

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“…These data also suggest that either oxygen availability was not compromised in these embryos, or that the oxygen sensing system and/or its coupling to EPO production was not yet functional. Strong relationships among EPO mRNA, plasma EPO, hematocrit and hemoglobin have been previously reported when animals have been made hypoxic and EPO production is stimulated (Kitanaka et al, 1989;Koury et al, 1989;Tan et al, 1992;Fu et al, 1993;Darby et al, 1995).…”

Section: Discussionmentioning

confidence: 70%

“…Such a phenomenon was apparent in fetal rats wherein at days 15 and 16 of gestation liver EPO was uninfluenced by hypoxia but after day 17 underwent an age-related decline in controls and increased in oxygen-deprived rats (Clemons et al, 1986). Once oxygen-associated control of EPO mRNA expression is established, expression under normoxic conditions is quite low to unmeasurable (Koury et al, 1989;Eckardt et al, 1992;Fu et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…Such associations were not apparent in our studies. If on the contrary, hypoxia were occurring due to other mechanisms such as reduced utero-placental blood flow or placental inadequacies (Maier et al, 1994) in the presence of a functional EPO sensing and production system, one might expect -depending on the rapidity and severity of the hypoxia -to measure increased EPO mRNA (Fu et al, 1993;Darby et al, 1995;Lim et al, 1996), plasma EPO (Kitanaka et al, 1989;Fu et al, 1993;Maier et al, 1993Maier et al, , 1994, and concomitantly increased hemoglobin and hematocrit (Kitanaka et al, 1989;Fu et al, 1993). In some experimental paradigms due to a time-lag in the stimulus-response coupling, there are significant negative associations between plasma EPO or EPO mRNA expression and red blood cell mass (Walle et al, 1987;Koury et al, 1989) or hemoglobin (Kling et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

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Erythropoietin mRNA expression in pig embryos

Klemcke

Vallet

Christenson

et al. 2001

Animal Reproduction Science

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To address whether altered erythropoietin (EPO) synthesis might be involved in prenatal pig mortality, studies were conducted to measure porcine embryonic EPO mRNA expression during early gestation (days 24-40). Three pig models differing in embryonic survival from days 24-40 were investigated: intact white crossbred gilts (INT), white crossbred gilts that were unilaterally hysterectomized-ovariectomized before puberty and whose pregnant uterus constituted a crowded environment (UHO), and prolific, intact Meishan gilts (ME). A partial cDNA for porcine EPO, developed via reverse transcription and polymerase chain reaction procedures was used to generate a 32P-labeled probe for use in Northern analyses. In an initial study, embryonic liver EPO mRNA was greatest on day 24, decreased by day 30 (P<0.01), and was barely detectable by day 40. EPO mRNA expression was not influenced by pig model. Placental EPO mRNA expression was detectable in only 4 of 53 placentae examined. In a second study at day 35 of gestation, embryonic liver EPO mRNA expression was measured in the same three pig models and in two embryos of divergent weights from each gilt. Meishan embryos had lower (P<0.01) plasma immunoassayable EPO concentrations (P=0.04) and higher survival rates (87+/-2.7%) at day 35 than did white crossbred embryos (75+/-5%). Liver EPO mRNA expression did not differ among animal models, nor did plasma EPO or tissue EPO mRNA expression differ between large and small embryos. There was no apparent relationship between embryonic development, measured as embryonic and placental size, and plasma EPO concentrations or liver EPO mRNA expression. These results indicate that at the gestational ages examined, the embryonic liver is one source of plasma erythropoietin and suggest that at the ages sampled, EPO is not a limiting factor in embryonic development.

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The Hematopoietic System

Car¹,

Seelig²

2022

Schalm's Veterinary Hematology

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The sheep erythropoietin gene: molecular cloning and effect of hemorrhage on plasma erythropoietin and renal/liver messenger RNA in adult sheep

Cited by 22 publications

References 27 publications

Erythropoietin production by interstitial cells of hypoxic monkey kidneys

Erythropoietin production by interstitial cells of hypoxic monkey kidneys

Erythropoietin mRNA expression in pig embryos

The Hematopoietic System

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