The serological grouping of<i>Streptococcus lactis</i>(group N) and its relationship to<i>Streptococcus faecalis</i>

Shattock, P. M. F.; Mattick, A. T. R.

doi:10.1017/s0022172400012791

Cited by 45 publications

(16 citation statements)

References 24 publications

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“…The technique of Jones & Shattock (1960) was used for preparing Groups D and Q antisera. Group N antisera were prepared by using acetone-extracted ball-milled cocci as antigens (Shattock & Mattick, 1943).…”

Section: Serological Methodsmentioning

confidence: 99%

The Cellular Location of Antigens in Streptococci of Groups D, N and Q

Smith

Shattock

1964

Journal of General Microbiology

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SUMMARYThe absence of Group D antigen from the cell walls and protoplasts of Group D streptococci leads to the conclusion that this antigen is located between the protoplast membrane and the cell wall. The Group N antigen was likewise not present in cell walls of Group N streptococci and probably has a similar location to the Group D antigen. The Group Q strains examined possessed the Group D antigen in the cell contents although these strains did not physiologically correspond to any established Group D species. The antigen upon which Group Q is based is a cell-wall antigen.

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Section: Serological Methodsmentioning

confidence: 99%

The Cellular Location of Antigens in Streptococci of Groups D, N and Q

Smith

Shattock

1964

Journal of General Microbiology

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“…or killed in 0.25 yo formalin (Lancefield, 1933). Cocci twice washed in acetone and dried in vacuum over P,O, were then ground in a ball mill under cold water (Shattock & Mattick, 1943). The stock vaccines were diluted to the opacity of Brown's tube no.…”

Section: Serological Methodsmentioning

confidence: 99%

The Streptococci of Lancefield's Group E; Biochemical and Serological Identification of the Haemolytic Strains

Moreira-Jacob

1956

Journal of General Microbiology

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SUMMARY :The non-pathogenic beta-haemolytic streptococci from milk, forming Lancefield's group E, were physiologically defined as Streptococcus infrequens and S. subacidus. They were found to fall into four serological types. Fractionation with ethanol showed that both group-and type-specific fractions of these organisms are of polysaccharide nature which probably accounts for the success in using formamide extracts for the type-precipitin reactions. S. subacidus possesses two type antigens, only one of which is also found in S . infrequens.

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“…; the technique of these tests has been described by Shattock & Mattick (1943) and by Shattock & Hirsch (1947). All fifty-five strains tested were Gram-positive cocci in pairs and short chains, which clotted litmus milk.…”

Section: -2mentioning

confidence: 99%

Enumeration ofStreptococcus faecalis, with particular reference to polluted waters

Allen¹,

Pierce²,

Smith³

1953

Epidemiol. Infect.

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Factors affecting the growth ofStreptococcus faecalison glucose-yeast extract-sodium azide agar have been studied. Both the high temperature of incubation used (45° C.) and the presence of azide reduced the proportion of cells able to form colonies, the inhibitory action being much more marked with cultures which had become attenuated, either through age or through prolonged immersion in water, than with comparatively young and vigorous cultures. This inhibitory action was found to be largely overcome if the inoculum was subjected to a preliminary period of ‘resuscitation’, by incubating it with double-strength glucose broth before adding the azide-agar portion of the medium and allowing the mixture to set.Neutral red was so inhibitory to some strains ofStr. faecalisthat it could not be included in the medium. Phosphate, as the potassium salt at a concentration of 0.7%, if autoclaved with the remaining constituents of the medium, exerted a depressing effect on the counts. Added separately it showed no inhibitory action.The spinning-bottle technique (Allenet al.1952) was adapted forStr. faecalis. When used for samples containing a mixed flora the method, described in the Appendix, permitted the growth only ofStr. faecalis.This paper is published by permission of the Department of Scientific and Industrial Research.

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The serological grouping ofStreptococcus lactis(group N) and its relationship toStreptococcus faecalis

Cited by 45 publications

References 24 publications

The Cellular Location of Antigens in Streptococci of Groups D, N and Q

The Cellular Location of Antigens in Streptococci of Groups D, N and Q

The Streptococci of Lancefield's Group E; Biochemical and Serological Identification of the Haemolytic Strains

Enumeration ofStreptococcus faecalis, with particular reference to polluted waters

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