The sequence of a cDNA done coding for a novel kallikrein from mouse submaxillary gland

Fahnestock, Margaret; Brundage, Suzy; Shooter, Eric M.

doi:10.1093/nar/14.12.4823

Cited by 10 publications

(7 citation statements)

References 31 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The probe used in the Southern blot shown in Figure 8 is pMF-l (Fahnestock et al, 1986), a cDNA clone coding for a mouse kallikrein. The mouse kallikrein family contains approximately 25 members that share 80-90% sequence homology, whereas the rat contains fewer kallikrein genes (Mason et al, 1983).…”

Section: Resultsmentioning

confidence: 99%

“…Proteins were transferred from polyacrylamide gels to nitrocellulose. Filters were probed with rabbit antiserum directed against mouse 0NGF and aNGF (Darling et al, 1983;Fahnestock et al, 1986). Bound rabbit antibodies were detected with 125I-Staph A protein.…”

Section: Methodsmentioning

confidence: 99%

“…Plasmids. A cDNA probe for a mouse kallikrein was previously isolated (Fahnestock et al, 1986). The probe for mouse yNGF (Ullrich et al, 1984) was obtained from A. Ullrich at Genentech.…”

Section: Methodsmentioning

confidence: 99%

“…Ten to twenty micrograms of each RNA was electrophoresed through a formaldehyde-agarose gel and transferred to nitrocellulose (Thomas, 1980). Filters were prehybridized overnight and hybridized with the nicktranslated probe (Fahnestock et al, 1986) for 48 h at 42 °C in the presence of 50% formamide. Washes were at room temperature first in 2 X SSPE-0.1% SDS and then in 0.1 X SSPE-0.1% SDS.…”

Section: Methodsmentioning

confidence: 99%

“…DNA was electrophoresed through a 0.8% agarose gel and transferred to nitrocellulose (Thomas, 1980). Hybridization was carried out as for Northern analysis above, with a cDNA probe for a mouse kallikrein, pMF-1 (Fahnestock et al, 1986).…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

The high molecular weight nerve growth factor complex from Mastomys natalensis differs from the murine nerve growth factor complex

Darling¹,

Fahnestock²

1988

Biochemistry

Self Cite

View full text Add to dashboard Cite

Both the male and the female of Mastomys natalensis, an African rat, have high levels of nerve growth factor (NGF) in their submaxillary glands. Mastomys high molecular weight (HMW) NGF was purified by gel filtration and ion-exchange chromatography and was compared with HMW NGF from the male mouse submaxillary gland. Mastomys HMW NGF sediments as a 5S species, does not exhibit esterase activity, and is more difficult to dissociate at acid pH than mouse 7S NGF. The biological activity could be isolated as a purified Mastomys beta NGF protein identical in size and charge with that purified from male mice. The N-terminal amino acid sequence of the first 20 residues was determined and found to differ from that of mouse only at residue 8. Western blotting of Mastomys 5S NGF using antiserum against mouse beta NGF indicates that the beta NGF subunit of Mastomys is very similar to that of the mouse. Southern blots using a mouse kallikrein probe also demonstrate the presence of a large kallikrein family in Mastomys similar to that in mouse, and Northern blots verify transcription of kallikreins in Mastomys submaxillary gland. SDS-PAGE and isoelectric focusing gels reveal a Mastomys subunit that comigrates with mouse alpha subunit. However, neither oligonucleotide probes directed against mouse alpha subunit RNA nor antibodies directed against mouse alpha NGF cross-react strongly with the Mastomys material. This indicates that the second subunit of the Mastomys complex is not very similar to the mouse alpha subunit.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Plasmids. A cDNA probe for a mouse kallikrein was previously isolated (Fahnestock et al, 1986). The probe for mouse yNGF (Ullrich et al, 1984) was obtained from A. Ullrich at Genentech.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

The high molecular weight nerve growth factor complex from Mastomys natalensis differs from the murine nerve growth factor complex

Darling¹,

Fahnestock²

1988

Biochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

The NGF complex from the African ratMastomys natalensis

et al. 1991

View full text Add to dashboard Cite

The 7S NGF complex from the male mouse submaxillary gland consists of the alpha, gamma, and beta subunits in the ratio alpha 2 gamma 2 beta. The beta (NGF) subunit contains all the known biolocial activity of 7S NGF. The alpha and gamma subunits are both members of glandular kallikrein gene family, yet only gamma subunit has protease activity. The gamma subunit plays a role in the processing of preproNGF to its mature form, while the role of the alpha subunit is not yet understood. Despite the fact that 7S NGF has been extensively characterized, no other NGF complex has been characterized, nor have the alpha or gamma subunits been observed in tissues which express NGF. We have therefore purified and characterized the NGF complex from the submaxillary glands of the rat Mastomys natalensis in order to more fully understand the roles of the alpha and gamma subunits. The NGF complex from M. natalensis contains subunits similar to those found in mouse 7S NGF. Although similar, there are significant differences between mouse and M. natalensis NGF complexes, especially in the degree of post-translational modification of the gamma and NGF subunits, the expression of esterase activity and the ease with which the complexes dissociate. Evidence is presented that suggests that the NGF complex from M. natalensis may consist of subunits in the ratio alpha 2 gamma beta. The amino acid sequence of the M. natalensis NGF suggests some, but not all, ways in which these differences arise.

show abstract

The NGF and kallikrein genes of mouse, the African rat Mastomys natalensis and man: their distribution and mode of expression in the salivary gland

Bowcock

Fahnestock

Goslin

et al. 1988

Molecular Brain Research

View full text Add to dashboard Cite

The sequence of a cDNA done coding for a novel kallikrein from mouse submaxillary gland

Cited by 10 publications

References 31 publications

The high molecular weight nerve growth factor complex from Mastomys natalensis differs from the murine nerve growth factor complex

The high molecular weight nerve growth factor complex from Mastomys natalensis differs from the murine nerve growth factor complex

The NGF complex from the African ratMastomys natalensis

The NGF and kallikrein genes of mouse, the African rat Mastomys natalensis and man: their distribution and mode of expression in the salivary gland

Contact Info

Product

Resources

About