1986
DOI: 10.1093/nar/14.12.4823
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The sequence of a cDNA done coding for a novel kallikrein from mouse submaxillary gland

Abstract: Mouse submaxillary gland contains many proteolytic enzymes, the most widely studied of which are the kallikreins. This gland also contains high levels of nerve growth factor (NGF), which is isolated as a complex of three subunits, alpha, beta, and gamma. We report here the cloning and sequence analysis of a novel kallikrein from mouse submaxillary gland. Antibodies directed against the alpha subunit precipitate the product of this clone, but do not precipitate the homologous gamma subunit. This new kallikrein … Show more

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Cited by 10 publications
(7 citation statements)
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References 31 publications
(40 reference statements)
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“…The probe used in the Southern blot shown in Figure 8 is pMF-l (Fahnestock et al, 1986), a cDNA clone coding for a mouse kallikrein. The mouse kallikrein family contains approximately 25 members that share 80-90% sequence homology, whereas the rat contains fewer kallikrein genes (Mason et al, 1983).…”
Section: Resultsmentioning
confidence: 99%
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“…The probe used in the Southern blot shown in Figure 8 is pMF-l (Fahnestock et al, 1986), a cDNA clone coding for a mouse kallikrein. The mouse kallikrein family contains approximately 25 members that share 80-90% sequence homology, whereas the rat contains fewer kallikrein genes (Mason et al, 1983).…”
Section: Resultsmentioning
confidence: 99%
“…Proteins were transferred from polyacrylamide gels to nitrocellulose. Filters were probed with rabbit antiserum directed against mouse 0NGF and aNGF (Darling et al, 1983;Fahnestock et al, 1986). Bound rabbit antibodies were detected with 125I-Staph A protein.…”
Section: Methodsmentioning
confidence: 99%
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