The Separation of the Major Ribonucleotides, Nucleosides, and Bases in Reverse-Phase Ion-Pair Chromatography

Pimenov, A M; Tikhonov, Yu. V.; Toguzov, P. T.

doi:10.1080/01483918608076686

Cited by 31 publications

(5 citation statements)

References 18 publications

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“…Purine nucleotides and their catabolites were separated using a reverse-phase ion-pair HPLC technique [8]. Since IMP and GMP as well as hypoxanthine and guanine could not be separated from each other, respectively, their contribution to the joint peaks was evaluated by simultaneous peak scanning considering the differences in the absorbance spectra of these compounds.…”

Section: Hplc Analysis Of Puke Compoundsmentioning

confidence: 99%

Mitochondrial metabolism of guanine nucleotides possible role of guanosine

Ziegler¹,

Dubiel²,

Pimenov³

et al. 1989

FEBS Letters

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The catabolism of intramitochondrial guanine nucleotides was examined. During 30 min incubation of rat liver mitochondria at 37°C in the presence of oligomycin and carboxyatractyloside, guanine and xanthine were formed and appeared in the medium. Under these conditions, the direct conversion of GMP to guanine by hypoxanthine-guanine phosphoribosyltransferase is suggested to be the main catabolic route within the organelles. Only very small amounts of guanosine were produced and detected both inside and outside the organelles. p4C]Guanosine and p*C]inosine were taken up by the mitochondria. Therefore, guanosine is suggested to be a precursor of intramitochondrial guanine nucleotides.

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Section: Hplc Analysis Of Puke Compoundsmentioning

confidence: 99%

Mitochondrial metabolism of guanine nucleotides possible role of guanosine

Ziegler¹,

Dubiel²,

Pimenov³

et al. 1989

FEBS Letters

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“…In terms of analyzing mononucleotides and dinucleotides by HPLC, an ODS [C(18)] column and acetonitrile with phosphate buffer have been most widely used. 21,22 The pH value of the mobile phase was varied from 3 to 7. Although literature demonstrated good resolution and high sensitivity in the conditions, phosphate buffer would suppress mass signal and decrease mass sensitivity in the HPLC-ESI-MS analysis.…”

Section: Resultsmentioning

confidence: 99%

Detection and Sequence Identification of Dinucleotides Produced from N‐Phosphoryl Alanine and Four Nucleosides by HPLC‐ESI‐MS/MS

et al. 2008

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The products of the model reaction between N- (O,O-diisopropyl phosphoryl) alanine (DIPP-Ala) and four nucleosides (adenosine, uridine, cytidine and guanosine) were studied by HPLC-ESI-MS/MS. The results showed that different mononucleotides and dinucleotides were produced. The sequences of dinucleotides formed from the DIPP-Ala and four nucleosides were identified. The results revealed that the c-ions from backbone fragmentation of the dinucleotides could be considered as the sequence-diagnostic ions. It was firstly testified that the diagnostic c-ions in positive and negative modes could be applied to identify the sequence of dinucleotide products formed in the reaction system.

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“…An ion-pairing reverse-phase HPLC was used to improve resolution and provide means to quantify RNA derivatives according to the method of Pimenov et al, 1986. Portions (10 µL) of water soluble fractions were injected on a Novopak C 18 column (Waters, 4 µm, 3.9 mm ϫ 150 mm).…”

Section: Rp-hplcmentioning

confidence: 99%

“…A Waters HPLC system (Miliford, MA, USA) consisted of a 600 E System Controller, a U6K Injector, a 486 Tunable Absorbance UV Detector and a Millenium 2010 Chromatography Manager, was used to detect ribonucleotides. An ion-pairing reverse-phase HPLC was used to improve resolution and provide means to quantify RNA derivatives according to the method of Pimenov et al, 1986. Portions (10 µL) of water soluble fractions were injected on a Novopak C 18 column (Waters, 4 µm, 3.9 mm ϫ 150 mm).…”

Section: Rp-hplcmentioning

confidence: 99%

Enzymatic Production of Ribonucleotides from Autolysates of Kluyveromyces marxianus Grown on Whey

Belem

Gibbs

Lee

1997

Journal of Food Science

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After 20h fermentation of medium containing 5% (w/v) dehydrated whey, at 30ЊC, pH 4.5, yeast cells were harvested, diluted in 0.1M KH 2 PO 4 , and autolyzed at different pHs (6.5-7.5) and temperatures (45-55ЊC). Phosphodiesterase (0.2-1.0% w/v, 65ЊC, pH 6.5, 6h) and adenyl deaminase (0.5-1.0% w/v, 60ЊC, pH 5.5, 4h) were added to the autolysates. After heat treatment (100ЊC, 15 min), samples were analyzed by RP-HPLC and LC/MS. Production of 5'-ribonucleotides was maximized at 50ЊC, pH 6.5. Yields of 5'-AMP (800 µg/g of biomass) and 5'-GMP (2000 µg/g) increased considerably after addition of 1.0% phosphodiesterase. 5'-IMP increased only after addition of 1.0% adenyl deaminase.

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The Separation of the Major Ribonucleotides, Nucleosides, and Bases in Reverse-Phase Ion-Pair Chromatography

Cited by 31 publications

References 18 publications

Mitochondrial metabolism of guanine nucleotides possible role of guanosine

Mitochondrial metabolism of guanine nucleotides possible role of guanosine

Detection and Sequence Identification of Dinucleotides Produced from N‐Phosphoryl Alanine and Four Nucleosides by HPLC‐ESI‐MS/MS

Enzymatic Production of Ribonucleotides from Autolysates of Kluyveromyces marxianus Grown on Whey

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