Detection and Sequence Identification of Dinucleotides Produced from N‐Phosphoryl Alanine and Four Nucleosides by HPLC‐ESI‐MS/MS

Abstract

The products of the model reaction between N- (O,O-diisopropyl phosphoryl) alanine (DIPP-Ala) and four nucleosides (adenosine, uridine, cytidine and guanosine) were studied by HPLC-ESI-MS/MS. The results showed that different mononucleotides and dinucleotides were produced. The sequences of dinucleotides formed from the DIPP-Ala and four nucleosides were identified. The results revealed that the c-ions from backbone fragmentation of the dinucleotides could be considered as the sequence-diagnostic ions. It was firstly testified that the diagnostic c-ions in positive and negative modes could be applied to identify the sequence of dinucleotide products formed in the reaction system.

ESI-MSnstudy on the fragmentation of protonated cyclic-dipeptides

“…However, identification of the structures of cyclic dipeptides in foods and beverages is still at a low output manipulation because of the limited availability of standards [6]. Electrospray ionization multistage mass spectrometry (ESI-MS n ) using low-energy collision-induced dissociation (CID) has been used as a powerful analytical method for investigating various kinds of organic and inorganic compounds with excellent sensitivity [8,9]. For full structure-elucidation of their mass data, it is necessary to understand all the gas-phase chemistry involved in each fragmentation step and to clarify the relationships between all the different possible pathways.…”