The Separation and Isolation of Particular Biochemical Mutants of Neurospora by Differential Germination of Conidia, Followed by Filtration and Selective Plating

Woodward, Val; Zeeuw, John R. De; Srb, Adrian M.

doi:10.1073/pnas.40.3.192

Cited by 98 publications

(21 citation statements)

References 2 publications

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“…Filtration and subsequent plating were made in accordance with the filtration-concentration technique of Woodward et al (1954) as revised by Case (1963). Thus, 257 is mutants which form colonies at 25°C on Fries minimal medium but are unable to form colonies at 35°C were isolated.…”

Section: Resultsmentioning

confidence: 99%

MACROMOLECULE SYNTHESIS AND GERMINATION OF CONIDIA IN TEMPERATURE SENSITIVE MUTANTS OF <i>NEUROSPORA CRASSA</i>

Inoue

Ishikawa

1970

The Japanese journal of genetics

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Section: Resultsmentioning

confidence: 99%

MACROMOLECULE SYNTHESIS AND GERMINATION OF CONIDIA IN TEMPERATURE SENSITIVE MUTANTS OF <i>NEUROSPORA CRASSA</i>

Inoue

Ishikawa

1970

The Japanese journal of genetics

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“…Secondly, experiments were done to see whether CO, mutants could be isolated as such from wild-type Neurospora by using a modification of the filtration-enrichment method (Woodward, de Zeeuw & Srb, 1954;Catcheside, 1954). This second method depended on the fact that wild-type conidia grew vigorously in minimal liquid medium swept through with a current of C0,-free air, whereas conidia from CO, mutants did not, even when in mixed suspension with wild-type conidia.…”

Section: Resultsmentioning

confidence: 99%

Some Carbon-Dioxide Requiring Mutants of Neurospora crassa

Broadbent

Charles

1965

Journal of General Microbiology

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SUMMARYThis paper describes some Neurospora mutants which grow on minimal medium when the gas-phase contains 30 yo CO, (v/v); they are referred to as 'CO, mutants'. One is an arginine mutant (arg-2; 33442) having a mutation at a different locus from the other arginine CO, mutant (arg-3; 30300) ; a second (arg-11 ; 30820) requires arginine + a purine + a pyrimidine for growth in the absence of CO,; a third (44601) requires arginine + a purine + a pyrimidine + a carboxylic acid for growth in the absence of CO,.The mutations in mutants 30820 and 44601 are allelic and the carboxylic acid requirement of mutant 44601 is attributable to its genetic background. The remaining CO, mutants described here were isolated directly from wild-type Neurospora, and their nutritional requirements in the absence of CO, subsequently determined. Two of these mutants, which are allelic, are adenine-requiring mutants sited at the adenine-3 locus, although the adenine-3A and -3B mutants 38709 and Y 112-M2 are not CO, mutants. Another two of these new CO, mutants, which may be allelic, require carboxylic acids in the absence of CO,; these mutants are similar in their nutritional requirements to the succinate mutants described by Lewis (1948) and Strauss (1956).

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“…Strain cs67 was isolated from such a suspension of R21 conidia following filtration enrichment (Woodward, De Zeeuw & Srb, 1954). The standard techniques of Pontecorvo et al (1953) were used.…”

Section: Methodsmentioning

confidence: 99%

Cytoplasmic Inheritance of a Cold-sensitive Mutant in Aspergillus nidulans

Waldron¹,

Roberts²

1973

Journal of General Microbiology

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Cold-sensitive mutants of bacteria are a source of strains defective in the assembly of ribosomes (Guthrie, Nashimoto & Nomura, 1969; Tai, Kessler & Ingraham, 1969). A study of cold-sensitive mutants in Aspergillus nidulans was undertaken in the hope that it would provide ribosomal mutants. Cold-sensitive (cs) mutants were isolated, and when crossed with wild-type the majority of 32 mutants tested segregated as single-gene mutations.One mutant, cs67, showed non-Mendelian segregation of its cold-sensi tive character in such a cross. This paper presents further results demonstrating the extranuclear inheritance of cs67. METHODSThe initial strains of Aspergillus nidulans used in this work were translocation-free. R21 (pabaAr Y A I ) carries the nuclear markers for p-aminobenzoic acid requirement and yellow conidia; ~1 5 3 (wA3; pyroAq) the nuclear markers for white conidia and pyridoxine requirement. RESULTSGrowth properties of cs67. At 37 "C growth of cs67 on solid media is indistinguishable from that of wild-type. The mutant forms only small colonies when incubated at 20 "C.The mutation is not specific to the germination stage since cs67 fails to grow at 20 "C even after an initial overnight incubation at 37 "C. Mutant cs67 does not grow at 20 "C in response to nutritional supplements, nor to molar concentrations of KCl or NaCl.The responses at 37 "C of cs67 to actidione (either 0.1 mg /ml or 2 mg/ml) or sodium deoxycholate (0.08 %) are the same as wild-type.Somatic stability of cs67. When conidia of cs67 were plated and incubated at 37 "C the resulting colonies showed no evidence of vegetative sectoring over several subcultures. Fourteen single-colony isolates from one of these subcultures were tested and all had precisely the same growth properties as the original isolate. Three of these clones (cs67-I, 1x67-2 and cs67-3) were used in the crosses and heterokaryon tests described below, in addition to the original strain (cs67-0).Crosses between cs67 and wild-type. Progeny from individual hybrid perithecia were tested for the segregation of the four nuclear markers and cold-sensitivity. In all cases the nuclear markers segregated as expected, the ratio of mutant to wild-type alleles being I:I (P > 0.05). However, the progeny from any one perithecium were either all cold-sensitive

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The Separation and Isolation of Particular Biochemical Mutants of Neurospora by Differential Germination of Conidia, Followed by Filtration and Selective Plating

Cited by 98 publications

References 2 publications

MACROMOLECULE SYNTHESIS AND GERMINATION OF CONIDIA IN TEMPERATURE SENSITIVE MUTANTS OF <i>NEUROSPORA CRASSA</i>

MACROMOLECULE SYNTHESIS AND GERMINATION OF CONIDIA IN TEMPERATURE SENSITIVE MUTANTS OF <i>NEUROSPORA CRASSA</i>

Some Carbon-Dioxide Requiring Mutants of Neurospora crassa

Cytoplasmic Inheritance of a Cold-sensitive Mutant in Aspergillus nidulans

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