The Selective Inhibitory Effect of Silver Nitroprusside Nanoparticles on Breast Tumor Growth

Abstract

A number of different metallic nanoparticles have been extensively investigated in recent years based on their diverse potential in biomedical and cancer applications, antibacterial activity, and chemical properties. Here, silver nitroprusside nanoparticles (AgNPs) were prepared from silver nitrate and sodium nitroprusside, and their anticancer activity was evaluated. AgNPs were prepared and characterized by Fourier transform infrared (FTIR), X-ray diffraction (DRX), scanning electron microscopy (SEM), X-ray dispersive energy spectroscopy (EDX), and transmission electron microscopy (TEM). In vitro tests were performed using two breast cancer cell lines, including a non-malignant breast epithelial cell line (MCF-10A) and a breast cancer cell line (MCF-7). The results obtained through cytotoxicity assays (MTT and resazurin) and bright-field microscopy revealed that AgNPs (3.0 mg/mL) exhibited specific selectivity for non-malignant breast epithelial cells. They were toxic to tumorigenic cells (MCF-7) with a lower selective toxicity to non-malignant breast epithelial cells (MCF-10A). In vivo imaging system (IVIS) imaging demonstrated the antitumor activity of AgNPs (3.0 mg/mL) compared to that of the control, and this was confirmed through histological analysis without any potential systemic toxicity. These results suggest that AgNPs possess potential anticancer applications in breast cancer treatment.

Silver nitroprusside nanoparticles for breast cancer therapy: in vitro and in vivo approach

“…It should be noted that the limited information on breast cancer research using silver nitroprusside nanoparticles is available in the public domain, which is a preprint version of a manuscript and has not been peer reviewed by a journal. 31…”

“…It should be noted that the limited information on breast cancer research using silver nitroprusside nanoparticles is available in the public domain, which is a preprint version of a manuscript and has not been peer reviewed by a journal. 31 Besides, the published report has several limitations and does not show systematic study on breast cancer using these nanoparticles. Therefore, a systematic study on breast cancer (both in vitro and in vivo) using detailed molecular mechanisms is urgently required, considering the current importance of breast cancer research in the healthcare system.…”