The Seeds of Doubt: Finding Seeds in Intriguing Places

Grillo, Federica; Campora, Michela; Cornara, Laura; Cascini, Alberta; Pigozzi, Simona; Migliora, Paola; Sarocchi, Francesca; Mastracci, Luca

doi:10.3389/fmed.2021.655113

Cited by 3 publications

(10 citation statements)

References 6 publications

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“…Many different types of seeds can be found in all gastrointestinal samples, as they often remain undigested due to the particular resistance of the seed's external cuticle to digestion. Various types of seeds have been reported in biopsy samples, polypectomy specimens and in various types of surgical specimens including appendicectomies, diverticular disease associated resections and cancers [59,60]. Among the most frequently found seeds, tomato seeds deserve more careful attention due to their complex morphology with glycoprotein-rich villi on the surface (which facilitate adhesion to the intestinal mucosa) making a misdiagnosis as parasites possible (Fig.…”

Section: Seedsmentioning

confidence: 99%

“Stranger things” in the gut: uncommon items in gastrointestinal specimens

et al. 2021

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Organic (such as parasites or vegetable remnants) and inorganic substances may be encountered during routine pathology diagnostic work up of endoscopic gastrointestinal biopsy samples and major resections, causing possible diagnostic conundrums for the young and not so young pathologists. The main aim of this review is the description of the most frequent oddities one can encounter as foreign bodies, in gastrointestinal pathology, on the basis of the current literature and personal experience. The types of encountered substances are divided into four principal categories: parasites (helminths such as Enterobius vermicularis, Strongyloides, Schistosoma, and Anisakis, and protozoa such as Entamoeba, Giardia and some intestinal coccidia); drugs and pharmaceutical fillers (found as deposits and as bystanders, innocent or not); seeds (possibly confused with worms) and plant remnants; pollutants (secondary to post-resection or post-biopsy contamination of the sample). An ample library of images is provided in order to consent easy referencing for diagnostic routine.

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Section: Seedsmentioning

confidence: 99%

“Stranger things” in the gut: uncommon items in gastrointestinal specimens

et al. 2021

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“…digested plant structures were categorized as the cases containing food residues. The types of the seeds observed in these cases were identified by comparing them with reference images of seeds and plants in the catalogues (5)(6)(7).…”

Section: Seeds or Parasites? Clinical And Histopathological Features ...mentioning

confidence: 99%

“…The relevant studies are compiled in Table I . Also, we reviewed the literature to identify studies about seed observed in appendectomy specimens without any date or language restriction and compiled the relevant studies in Table II (6,7,(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62).…”

Section: Seeds or Parasites? Clinical And Histopathological Features ...mentioning

confidence: 99%

“…In the following years, relatively few studies addressed the seeds, which may be attributed to the fact that the observation of seeds in the appendix lumen did not change the treatment plan or follow-up approaches, and thus that the studies aimed at such findings did not receive enough attention from researchers for publication purposes. To our best knowledge, only 63 cases have been described in the literature to date (6,7,(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62). The details of these cases are summarized in Table II.…”

Section: B Amentioning

confidence: 99%

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Seeds or parasites? clinical and histopathological features of seeds and parasites in the appendix

Issın¹,

Demir²,

Şimşek³

et al. 2022

TJPATH

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Objective: Parasites and plant seeds may both be found in appendectomy specimens. Each plant seed has a different appearance and can thus exhibit wide variations under the microscope. Fragmented seeds may histologically mimic parasites. The differential diagnosis between seeds and parasites can be challenging in such cases. This study aimed to determine the incidence of parasites, seeds, and foreign bodies in appendectomy materials and highlight the most characteristic histopathological features associated with these structures. Material and Method:In this study, pathology slides of 9,480 patients, who underwent appendectomy between 2010 and 2021, were reviewed, and cases that contained parasites, seeds, or foreign bodies were identified. We reviewed the literature on seeds and parasites in appendectomy specimens. Results:Parasites were observed in 56 (0.6%) cases. Of these cases, 45 had Enterobius vermicularis (80%), and 11 had Taenia subspecies (20%). Plant seeds were observed in 47 cases (0.5%), and were macroscopically recognizable in 5 of them as olive, lemon, and cherry seeds. Parasites and seeds were usually observed in the lumen of appendix vermiformis, filled with abundant fecal materials. Conclusion:Seeds are seen in approximately 0.5% of the appendectomy specimens. Though rarely seen, the fragmented seed appearance may cause diagnostic difficulties. In this context, the key morphological features of parasites and plant seeds outlined in this study may be helpful in their differential diagnosis.

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“…Notably, the brilliant staining of the eggs of E. vermicularis, raises the possibility that RGB trichrome staining could be a useful tool for the identification of eggs from different nematode and/or other helminth species, and deserves comparative studies for this purpose. While studying the appendix samples to assess E. vermicularis staining, we noticed the presence of occasional undigested seeds and plant residuals that can be eventually confused with parasites and, in fact, several studies have addressed this issue [30][31][32][33] . In this context, we decided to investigate the staining outcomes of seeds and plant tissue remnants after application of RGB trichrome as a method that contributes to its differentiation from parasites.…”

Section: Enterobius Vermicularismentioning

confidence: 99%

Application of RGB trichrome staining to the study of human parasites

Gonzalez-Luna

Gaytán

2022

Preprint

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Human parasitic infections are major contributors to global disease load, compromising the human life and resulting in considerable morbidity and mortality worldwide. However, many parasitic diseases have been neglected and little investigated in western medicine. Although recently developed molecular techniques have revolutionized the taxonomy of parasites and the parasitic disease diagnosis, histopathology still remains a powerfull tool for the analysis of parasitic diseases, allowing direct observation of the parasite thus providing information about the morphological features of the parasite ifself, and revealing tissue alterations at the parasite-host interface. The recently developed RGB trichrome (acronym for the primary dye components, picrosirius Red, fast Green, and alcian Blue) stains the main components of the extracellular matrix, specifically collagens and proteoglycans. We have applied the RGB tricrome staining to human tissues infected by the main three classes of human parasites: Protozoa (Leishmania donovanii and Toxoplasma gondii), helminths (Trichinella spiralis, Enterobius vermicularis, Dirofilaria spp. and Echinococcus granulosus) and ectoparasites (Demodex folliculorum and Demodex brevis). Trichrome stain results in detailed staining of the parasite microanatomical structure, and highlights host tissue alterations such as granulomatous inflammation, immune cell infiltrate, or increased amount of collagen as a sign of parasite-induced fibrosis. Yet, the use of RGB trichrome, as a complement of hematoxylin and eosin staining, provides additional valuable information to assess parasitic infection histopathology.

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The Seeds of Doubt: Finding Seeds in Intriguing Places

Cited by 3 publications

References 6 publications

“Stranger things” in the gut: uncommon items in gastrointestinal specimens

“Stranger things” in the gut: uncommon items in gastrointestinal specimens

Seeds or parasites? clinical and histopathological features of seeds and parasites in the appendix

Application of RGB trichrome staining to the study of human parasites

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