The Secretion of Oestrogen and Progesterone in Response to Luteinizing Hormone by Pig Luteal Tissue under Superfused Conditions

Leask, J.T.S.; Alam, M.; Watson, John

doi:10.1042/bst0010498

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A possible explanation for this variation in the present system has been obtained in recent experiments in which the binding of 125I-labelled-LH to porcine luteal tissue was studied under identical superfusion conditions (Leask, Watson & Anderson, 1974). When a pulse of 125I-labelled-LH was injected into the incubation chamber, considerable differences in binding of the radio¬ active gonadotrophin were observed, whereas infusion of the LH over short periods gave consistent binding.…”

Section: Discussionmentioning

confidence: 88%

Superfusion in Vitro in the Study of Ovarian Steroidogenesis

Watson¹,

Leask²

1975

Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

A method for the continuous superfusion of porcine corpus luteum tissue is described which readily allows both the introduction of regulatory factors to the incubating tissue, and sampling of the tissue. Oestrogen (principally oestradiol) and progestin (principally progesterone) can be measured for up to 24 h in the superfusate from corpora lutea of all ages, and the secretion of both steroids is stimulated by the addition of luteinizing hormone. The pattern of response of both steroids to a pulse of gonadotrophin was similar in that a rapid transient increase in secretion occurred followed some time later by a secondary and more prolonged response. A second pulse of gonadotrophin introduced 6 h after the first also stimulated steroid secretion, indicating that during superfusion in vitro the porcine corpus luteum does not become refractory to the steroidogenic effect of gonadotrophin.

show abstract

Section: Discussionmentioning

confidence: 88%